DDX49 is an RNA helicase that affects translation by regulating mRNA export and the levels of pre-ribosomal RNA

Awasthi, Sharad; Verma, Mahesh; Mahesh, Arun; Khan, Mohd. Imran; Govindaraju, Gayathri; Rajavelu, Arumugam; Chavali, Pavithra L.; Chavali, Sreenivas; Dhayalan, Arunkumar

doi:10.1093/nar/gky231

Cited by 32 publications

(35 citation statements)

References 56 publications

(63 reference statements)

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“…Both DDX39B and DDX49 are required for efficient regulation of pre-ribosomal RNA Recently, we showed that DDX49 affects the pre-ribosomal 47S RNA levels [16] and here we find that DDX39B has a similar function. Since both DDX39B and DDX49 displayed overlapping roles in regulating pre-ribosomal RNA levels, we investigated the functional relationship between these two RNA helicases.…”

Section: Ddx39b Regulates Cell Proliferation and Is Elevated In Diversupporting

confidence: 74%

“…Our findings presented here on the regulation of pre-ribosomal levels, adds DDX39B to this list. Recently, we reported that DDX49 regulates the steady state levels of pre-ribosomal 47S RNA [16]. Here, we show that both DDX49 and DDX39B are essential for efficient regulation of 47S rRNA in the cell.…”

Section: Discussionmentioning

confidence: 53%

“…Interestingly, both the knock-down and overexpression of DDX39B leads to the nuclear retention of poly (A) containing mRNAs indicating that the mRNA export activity of DDX39B is dosage-sensitive [7]. Investigations in different cell types such as HeLa and HEK293 cells by others [6] and us [16] ( Figure S1) yield similar results, implying this phenomenon could be cell-type independent. On the contrary, overexpression of DDX39B has been shown to increase the global translation [17].…”

Section: Introductionmentioning

confidence: 61%

“…The full length human DDX39B (NM_004640.6) was PCR amplified from cDNA prepared from HEK293 cells and cloned in pEGFP-C1 vector (Clontech) using the restriction sites BamHI and XhoI (DDX39B) to generate pEGFP-DDX39B construct. The generation of pEGFP-DDX49 and pGEX-DDX39B constructs was described previously [16].…”

Section: Cloningmentioning

confidence: 99%

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DDX39B promotes translation through regulation of pre-ribosomal RNA levels

et al. 2018

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DDX39B, a DExD RNA helicase, is known to be involved in various cellular processes such as mRNA export, splicing and translation. Previous studies showed that the overexpression of DDX39B promotes the global translation but inhibits the mRNA export in a dominant negative manner. This presents a conundrum as to how DDX39B overexpression would increase the global translation if it inhibits the nuclear export of mRNAs. We resolve this by showing that DDX39B affects the levels of pre-ribosomal RNA by regulating its stability as well as synthesis. Furthermore, DDX39B promotes proliferation and colony forming potential of cells and its levels are significantly elevated in diverse cancer types. Thus, increase in DDX39B enhances global translation and cell proliferation through upregulation of pre-ribosomal RNA. This highlights a possible mechanism by which dysregulation of DDX39B expression could lead to oncogenesis.

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Section: Ddx39b Regulates Cell Proliferation and Is Elevated In Diversupporting

confidence: 74%

Section: Discussionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 61%

Section: Cloningmentioning

confidence: 99%

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DDX39B promotes translation through regulation of pre-ribosomal RNA levels

et al. 2018

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“…We found that down-regulation of DDX49 in NSCLC cells significantly suppressed NSCLC cell growth and migration. Sharad Awasthi also proposed a similar conclusion: DDX49 displays a robust ATPase and RNA helicase activity and it is involved in the export of poly (A) + mRNAs to cytoplasm, DDX49 affects cell proliferation and its aberrant expression might have oncogenic potential 6 . We found that these genes were more likely involved in the PI3K/Akt pathway, cell and metabolic pathways, and focal adhesion by KEGG pathway analysis.…”

Section: Discussionmentioning

confidence: 84%

DDX49 is a novel biomarker and therapeutic target for lung cancer metastases

Lian

Xiang

Peng

et al. 2019

J Cellular Molecular Medi

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The identification of lymph node metastases is important for the diagnosis, treatment and prognosis of patients with lung cancer. We found DDX49 was associated with the lymph node metastases in lung cancer by the Akt/β‐catenin pathway. Transcriptome sequencing, bioinformatics analysis, quantitative RT‐PCR, cell transfection and the Cancer Genome Atlas (TCGA) data set were used to identify DDX49 responsible for lymph node metastases. A Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was used to explore the possible molecular mechanism in experimental cell. The DDX49 gene was correlated significantly with lymph node metastases of lung cancer. The knockdown of DDX49 inhibited the cell proliferation and migration in PC‐9 and H460 cells. The mechanism research found downexpression of DDX49 decreased the Akt/β‐catenin pathway in lung cancer cell. In vivo experiments showed that DDX49 promoted the proliferation and metastases of lung cancer cells by increasing the Akt/β‐catenin pathway. These findings suggested that DDX49 may be useful as a novel biomarker of lymph node metastases and therapeutic target for lung cancer metastasis.

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Three RNA helicase DDX genes are essential for the development and oocyte maturation in Laodelphax striatellus

Ma,

Zhang,

Chen

et al. 2024

Pest Management Science

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BackgroundDEAD‐box protein (DDX) is a member of the DDX RNA helicase family that exerts multiple functions in RNA metabolism, cell cycle, tumorigenesis, signal pathway, and fertility, particularly in mammals. Nevertheless, the biological functions of DDXs in insects have not been fully resolved and attracted increasing attention these years. Laodelphax striatellus (Hemiptera) is a notorious rice pest through feeding on rice sap and transmitting plant viruses. In this study, we aim to elucidate the functional characterization of DDXs in L. striatellus, and to exploit potential target genes for the development of pest control strategies.ResultsIn this study, we characterized the expression patterns of LsDDX6, LsDDX47, and LsDDX51 in planthoppers and analyzed their conserved motifs. These genes were found to be expressed in all tissues and developmental stages examined, with significantly higher transcript levels observed in the ovary. Knockdown of LsDDX6, LsDDX47, and LsDDX51 resulted in an obvious lethal phenotype in nymphs and abnormal ovarian development in adults. Furthermore, a total of 27 DDXs were identified in L. striatellus, and most DDXs were highly expressed in ovary and structure analysis result revealed that all of the DDXs possessed nine motifs that were unique to the DDX family.ConclusionThe three DDX RNA helicases (LsDDX6, LsDDX47, and LsDDX51) are essential for both survivorship and reproduction in L. striatellus. Considering a total number of 27 DDXs identified in L. striatellus, they might serve as promising candidates for application in RNAi‐based control of this destructive pest. © 2024 Society of Chemical Industry.

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DDX49 is an RNA helicase that affects translation by regulating mRNA export and the levels of pre-ribosomal RNA

Cited by 32 publications

References 56 publications

DDX39B promotes translation through regulation of pre-ribosomal RNA levels

DDX39B promotes translation through regulation of pre-ribosomal RNA levels

DDX49 is a novel biomarker and therapeutic target for lung cancer metastases

Three RNA helicase DDX genes are essential for the development and oocyte maturation in Laodelphax striatellus

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