De la cellule au tissu : le magnétisme auxiliaire de la biomédecine

Frascá, G; Gazeau, Florence; Wilhelm, Claire

doi:10.1051/refdp/2011236

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…[13][14][15][16] Based on the magnetic properties of iron oxide, magnetically labeled cells of interest are controlled and driven by an external magnetic field. 17,18 Such research has been used for cardiovascular tissue construction, 19 tubular structures, 20 and 3D culture systems of the hepatocyte cell line. 21 Researchers in the field of analytical chemistry have also introduced the use of magnetic nano (or micro) particles in their developments of new analytical tools in numerous applications.…”

Section: Introductionmentioning

confidence: 99%

Hepatocyte-based flow analytical bioreactor for online xenobiotics metabolism bioprediction

2017

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The research for new in vitro screening tools for predictive metabolic profiling of drug candidates is of major interest in the pharmaceutical field. The main motivation is to avoid late rejection in drug development and to deliver safer drugs to the market. Thanks to the superparamagnetic properties of iron oxide nanoparticles, a flow bioreactor has been developed which is able to perform xenobiotic metabolism studies. The selected cell line (HepaRG) maintained its metabolic competencies once iron oxide nanoparticles were internalized. Based on magnetically trapped cells in a homemade immobilization chamber, through which a flow of circulating phase was injected to transport nutrients and/or the studied xenobiotic, off-line and online (when coupled to a high-performance liquid chromatography chain) metabolic assays were developed using diclofenac as a reference compound. The diclofenac demonstrated a similar metabolization profile chromatogram, both with the newly developed setup and with the control situation. Highly versatile, this pioneering and innovative instrumental design paves the way for a new approach in predictive metabolism studies.

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Section: Introductionmentioning

confidence: 99%

Hepatocyte-based flow analytical bioreactor for online xenobiotics metabolism bioprediction

2017

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“…During the last decade, an original and promising strategy to build tridimensional culture and tissue engineering has emerged by using magnetic nanoparticles and magnetic force [19][20][21]. An external magnetic field such as magnets is able to control and drive magnetically labeled cells, resulting in an influence on their organization and their migration [22,23]. This technology covers a wide range of applications from construction of tubular structures and cardiovascular tissues (localization of endothelial cells in magnetized stented vessels included) [24][25][26] to 3D culture of hepatocytes [27].…”

Section: Introductionmentioning

confidence: 99%

Interaction between Iron Oxide Nanoparticles and HepaRG Cells: A Preliminary In Vitro Evaluation

Helvenstein

Stanicki

Laurent

et al. 2015

Journal of Nanomaterials

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Nowadays, the use of iron oxide nanoparticles is widespread to label cells for magnetic resonance imaging tracking. More recently, magnetic labeling provides promising new opportunities for tissue engineering by controlling and manipulating cells through the action of an external magnetic field. The present work describes nonspecific labeling of metabolically competent HepaRG hepatocytes with anionic iron oxide nanoparticles. An interaction was observed between nanoparticles and studied cells, which were easily attracted when exposed to a magnet. No cytotoxicity was detected in the hepatocytes after 24 hours of incubation with iron oxide nanoparticles. Impact on HepaRG metabolization activity was assessed. Although a slight decrease in the metabolite generation was observed after exposure to nanoparticles (2 mM in iron), the enzymatic capacity was maintained. These results pave the way for 3D cultures of magnetic labeled HepaRG cells by using a magnetic field.

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