De novo analysis of the Adelphocoris suturalis Jakovlev metathoracic scent glands transcriptome and expression patterns of pheromone biosynthesis-related genes

Luo, Jing; Li, Xiangyang; Zhang, Poyao; Chen, Long-jia; Gao, Qiao; Ma, Weihua; Chen, Lizhen; Lei, Chaoliang

doi:10.1016/j.gene.2014.09.004

Cited by 19 publications

(14 citation statements)

References 51 publications

(66 reference statements)

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“…Based on the A. suturalis transcriptome data (Luo et al ., ), we isolated two cDNAs encoding putative Asutdes sequences and designated them as Asutdes1 and Asutdes2 . Although the 5′ untranslated region of the 1863‐bp ( Asutdes1 ) and 1780‐bp ( Asutdes2) transcripts differed in size and sequence composition (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…Given the structural similarities between moth SPs and those described in A. suturalis , we hypothesized that the pheromone biosynthetic pathways might also be similar. We initially identified four genes in A. suturalis as candidate pheromone biosynthesis genes based on sequence homology with key enzymes in moth SP biosynthesis (Luo et al ., ). In this study, we found that silencing Asutdes expression potently suppressed the sexual attractiveness of males to females in both laboratory (Y‐tube olfactometer) and field conditions.…”

Section: Discussionmentioning

confidence: 99%

“…For first‐strand cDNA synthesis, 1 μg RNA was reverse transcribed using PrimeScript TM RT Master Mix (perfect real time) (Takara, Kyoto, Japan). Full‐length Asutdes1 and Asutdes2 were amplified using primers (Table ) designed based upon A. suturalis transcriptome data (Luo et al ., ), cloned into a pEASY‐T1 Simple Cloning Kit (TransGen, Beijing, China) and sequenced. The ExPASy translate tool (http://web.expasy.org/translate/) was used to deduce the amino acid sequence of the protein encoded by the Asutdes ORF.…”

Section: Methodsmentioning

confidence: 97%

“…Thus, it can be hypothesized that pheromone biosynthesis in A. suturalis is catalysed by homologues of moth pheromone biosynthetic enzymes. To identify potential A. suturalis SP biosynthesis‐related genes, we mined previous A. suturalis MTG Illumina sequencing data (Luo et al ., ) for transcripts with high sequence similarity to key moth pheromone biosynthesis enzymes. We identified four candidate genes that showed higher expression in female A. suturalis MTGs during the calling period: fatty acyl‐coenzyme A (CoA) reductase , desaturase ( des ), acetyltransferase and alcohol oxidase .…”

Section: Introductionmentioning

confidence: 99%

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Knockdown of a metathoracic scent gland desaturase enhances the production of (E)‐4‐oxo‐2‐hexenal and suppresses female sexual attractiveness in the plant bug Adelphocoris suturalis

Luo

et al. 2017

Insect Molecular Biology

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Insect sex pheromones (SPs) are central to mate-finding behaviour, and play an essential role in the survival and reproduction of organisms. Understanding the roles, biosynthetic pathways and evolution of insect chemical communication systems has been an exciting challenge for biologists. Compared with Lepidoptera, little is known about the mechanisms underlying pheromone biosynthesis in Hemiptera. In this study, we isolated and characterized two new desaturase-like genes, termed Asutdes1 and Asutdes2, from Adelphocoris suturalis, an important agricultural pest in China. Although the two genes encode an identical protein, Southern blot analysis revealed that they are duplicated genes. The Asutdes2 transcript is more abundant than Asutdes1 in the tissues tested, in particular the metathoracic scent gland and fat body. Silencing Asutdes expression in females by injecting double-stranded RNA (dsAsutdes) against a portion of the coding sequence shared by the two genes enhanced the production of (E)-4-oxo-2-hexenal, a component of the A. suturalis SP blend, and dramatically suppressed the sexual attractiveness of A. suturalis females. We conclude that dsAsutdes is associated with the SP biosynthetic pathway in A. suturalis.

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Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Knockdown of a metathoracic scent gland desaturase enhances the production of (E)‐4‐oxo‐2‐hexenal and suppresses female sexual attractiveness in the plant bug Adelphocoris suturalis

Luo

et al. 2017

Insect Molecular Biology

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“…These species are highly polyphagous and attack a broad range of cultivated crops, such as cotton, beans, alfalfa, vegetables, and fruit crops [10]. Both the nymphs and adults of these species suck plant sap from cotton flower buds, tender shoots, and buds, resulting in abscission, wilting, abnormal growth, and losses in lint yield and quality [11].…”

Section: Introductionmentioning

confidence: 99%

Integrative Proteomic and Metabolomic Analysis Reveals the Cotton Plant Defense Mechanisms Induced by Insect (<em>Adelphocoris suturalis</em> Jakovlev) Feeding

Lü¹,

Zhang²,

Luo³

et al. 2018

Preprint

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Cotton (Gossypium hirsutum Linn.) is widely cultivated in China. The polyphagous insect Adelphocoris suturalis (Jakovlev) is a serious insect pest in cotton growing regions. Plants have evolved sophisticated systems to cope with herbivore attacks. However, the cotton defense mechanisms induced by A. suturalis feeding have lagged behind. We carried out untargeted proteomic analysis using the iTARQ technique and metabolomics based on LC-MS/MS analysis of cotton leaves fed upon by A. suturalis. Proteomic analysis identified 775 upregulated proteins and 477 downregulated proteins in plants that were infested by A. sututralis compared to the controls. Metabolomic analysis identified 50 differentially expressed metabolites in the positive ion mode and 14 in the negative ion mode compared to the controls. The tryptophan metabolism pathway was significantly changed in both the positive and negative ion mode in the metabolomics analysis. The alpha-linolenic acid pathway was significantly changed in both the proteomic and metabolomics analyses. Furthermore, the result was validated by RT-qPCR analysis of 5 related genes involved in alpha-linolenic acid pathway. These results indicate that tryptophan metabolism and the alpha-linolenic acid pathway may be important in cotton defense against herbivores and would enhance our understanding of plant defenses induced by A. sututrali feeding.

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Metabolic pathways in the mixed segment of the wood-feeding termite Nasutitermes takasagoensis (Blattodea (Isoptera): Termitidae)

et al. 2016

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De novo analysis of the Adelphocoris suturalis Jakovlev metathoracic scent glands transcriptome and expression patterns of pheromone biosynthesis-related genes

Cited by 19 publications

References 51 publications

Knockdown of a metathoracic scent gland desaturase enhances the production of (E)‐4‐oxo‐2‐hexenal and suppresses female sexual attractiveness in the plant bug Adelphocoris suturalis

Knockdown of a metathoracic scent gland desaturase enhances the production of (E)‐4‐oxo‐2‐hexenal and suppresses female sexual attractiveness in the plant bug Adelphocoris suturalis

Integrative Proteomic and Metabolomic Analysis Reveals the Cotton Plant Defense Mechanisms Induced by Insect (<em>Adelphocoris suturalis</em> Jakovlev) Feeding

Metabolic pathways in the mixed segment of the wood-feeding termite Nasutitermes takasagoensis (Blattodea (Isoptera): Termitidae)

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