De novo analysis of the Tenebrio molitor (Coleoptera: Tenebrionidae) transcriptome and identification of putative glutathione S-transferase genes

Liu, Su; Shi, Xiaoxiao; Jiang, Yuan; Zhu, Zi-Jie; Qian, Ping; Zhang, Minjing; Yu, Hang; Zhu, Qi; Zhu, Zhiliang

doi:10.1007/s13355-014-0305-8

Cited by 13 publications

(6 citation statements)

References 39 publications

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“…The numbers of GST genes varied greatly across insect species. − Results showed that most species of Lepidoptera possess a large number of GST genes similar to Coleoptera, Diptera, and Orthoptera. But this number is far fewer in Hemiptera and Hymenoptera.…”

Section: Resultsmentioning

confidence: 99%

Glutathione S-Transferase Genes are Involved in Lambda-Cyhalothrin Resistance in Cydia pomonella via Sequestration

Liu

Wang

et al. 2022

J. Agric. Food Chem.

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Pest management is mostly accomplished by the use of insecticides. However, the overuse of insecticides has led to the development of resistance. Glutathione S-transferases (GSTs) are vital detoxification enzymes involved in insecticide resistance in insects. In this study, we report the involvement of GSTs in insecticide resistance to lambda-cyhalothrin in Cydia pomonella, a globally quarantined fruit pest. A total of 25 GST, including 22 cytosolic genes and 3 microsomal genes, are identified from the genome database of C. pomonella. These cytosolic genes are further classified into six classes, including four in delta, eight in epsilon, three in omega, three in sigma, one in theta, and one in zeta class, as well as two unclassified genes. The real-time quantitative polymerase chain reaction (RT-qPCR) shows that the majority of these genes are mainly expressed throughout the larval stage and in the midgut of the fourth-instar larvae. Exposure to an LD10 dose of lambda-cyhalothrin resulted in the upregulation of 17 GST genes. Moreover, mRNA levels of most GST genes, with the exception of CpGSTe6, CpGSTd2, CpGSTd4, and CpGSTz1, are considerably higher in a lambda-cyhalothrin-resistant population (ZW_R) than those of susceptible strains. Recombinant CpGSTd1, CpGSTd3, CpGSTe3, and CpGSTs2 can bind and metabolize lambda-cyhalothrin, with the highest metabolic rate observed for CpGSTd3 but no metabolite(s) was detected, supporting the role of GSTs in sequestration of lambda-cyhalothrin. Molecular dynamics simulation analysis indicates that key residues of hydrophobic pocket-derived lipophilic energy S(lipo) interactions with a hydrophobic pharmacophore of lambda-cyhalothrin are crucial for metabolism by CpGSTd3 and further lead to resistance. Our study is the first to experimentally confirm the involvement of GSTs in lambda-cyhalothrin resistance via sequestration and provides new insights into resistance management in C. pomonella.

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Section: Resultsmentioning

confidence: 99%

Glutathione S-Transferase Genes are Involved in Lambda-Cyhalothrin Resistance in Cydia pomonella via Sequestration

Liu

Wang

et al. 2022

J. Agric. Food Chem.

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“…RNA samples with an A260/A280 > 1.8, A260/A230 > 1.8, and RNA integrity number (RIN) > 8 were considered acceptable for library construction. Equal amounts of RNA from three different samples were pooled before cDNA library preparation, and mRNA enrichment, cDNA synthesis, fragmentation, adapter addition, selection of fragment size, PCR amplification, and transcriptomic sequencing were performed by staff at the Beijing Genome Institute (BGI; Shenzhen, China) as previously described by Liu et al [ 46 ] and Gu et al [ 47 ]. Briefly, mRNAs were purified from total RNA using magnetic beads with Oligo (dT) and cleaved into short sequences.…”

Section: Methodsmentioning

confidence: 99%

Gene co-expression network analysis reveals coordinated regulation of three characteristic secondary biosynthetic pathways in tea plant (Camellia sinensis)

Tai

Chun

et al. 2018

BMC Genomics

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BackgroundThe leaves of tea plants (Camellia sinensis) are used to produce tea, which is one of the most popular beverages consumed worldwide. The nutritional value and health benefits of tea are mainly related to three abundant characteristic metabolites; catechins, theanine and caffeine. Weighted gene co-expression network analysis (WGCNA) is a powerful system for investigating correlations between genes, identifying modules among highly correlated genes, and relating modules to phenotypic traits based on gene expression profiling. Currently, relatively little is known about the regulatory mechanisms and correlations between these three secondary metabolic pathways at the omics level in tea.ResultsIn this study, levels of the three secondary metabolites in ten different tissues of tea plants were determined, 87,319 high-quality unigenes were assembled, and 55,607 differentially expressed genes (DEGs) were identified by pairwise comparison. The resultant co-expression network included 35 co-expression modules, of which 20 modules were significantly associated with the biosynthesis of catechins, theanine and caffeine. Furthermore, we identified several hub genes related to these three metabolic pathways, and analysed their regulatory relationships using RNA-Seq data. The results showed that these hub genes are regulated by genes involved in all three metabolic pathways, and they regulate the biosynthesis of all three metabolites. It is notable that light was identified as an important regulator for the biosynthesis of catechins.ConclusionOur integrated omics-level WGCNA analysis provides novel insights into the potential regulatory mechanisms of catechins, theanine and caffeine metabolism, and the identified hub genes provide an important reference for further research on the molecular biology of tea plants.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4999-9) contains supplementary material, which is available to authorized users.

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“…P. antennata possessed a similar proportion of GSTs in the two clades (62.50%) with the other three cerambycid beetles A. glabripennis (71.43%) (Mason et al, 2016), R. horsfieldi (65.00%) and X. quadripes (66.67%) (Zhao et al, 2020b), suggesting the requirements for detoxifying a variety of toxic compounds. The extensive expression of Delta and Epsilon genes in abdomens of P. antennata further conferred their resistance to xenobiotics, similar to the GST genes in Tenebrio molitor (Liu et al, 2015), A. glabripennis (Scully et al, 2013) and X. quadripes (Zhao et al, 2020b). Beyond the detoxification, insect GSTs have been also implied to have olfactory roles responsible for the degradation of odorants (Rogers et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of candidate detoxification genes in Pharsalia antennata Gahan (Coleoptera: Cerambycidae)

et al. 2022

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The wood-boring beetles, including the majority of Cerambycidae, have developed the ability to metabolize a variety of toxic compounds derived from host plants and the surrounding environment. However, detoxification mechanisms underlying the evolutionary adaptation of a cerambycid beetle Pharsalia antennata to hosts and habitats are largely unexplored. Here, we characterized three key gene families in relation to detoxification (cytochrome P450 monooxygenases: P450s, carboxylesterases: COEs and glutathione-Stransferases: GSTs), by combinations of transcriptomics, gene identification, phylogenetics and expression profiles. Illumina sequencing generated 668,701,566 filtered reads in 12 tissues of P. antennata, summing to 100.28 gigabases data. From the transcriptome, 215 genes encoding 106 P450s, 77 COEs and 32 GSTs were identified, of which 107 relatives were differentially expressed genes. Of the identified 215 genes, a number of relatives showed the orthology to those in Anoplophora glabripennis, revealing 1:1 relationships in 94 phylogenetic clades. In the trees, P. antennata detoxification genes mainly clustered into one or two subfamilies, including 64 P450s in the CYP3 clan, 33 COEs in clade A, and 20 GSTs in Delta and Epsilon subclasses. Combining transcriptomic data and PCR approaches, the numbers of detoxification genes expressed in abdomens, antennae and legs were 188, 148 and 141, respectively. Notably, some genes exhibited significantly sexbiased levels in antennae or legs of both sexes. The findings provide valuable reference resources for further exploring xenobiotics metabolism and odorant detection in P. antennata.

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De novo analysis of the Tenebrio molitor (Coleoptera: Tenebrionidae) transcriptome and identification of putative glutathione S-transferase genes

Cited by 13 publications

References 39 publications

Glutathione S-Transferase Genes are Involved in Lambda-Cyhalothrin Resistance in Cydia pomonella via Sequestration

Glutathione S-Transferase Genes are Involved in Lambda-Cyhalothrin Resistance in Cydia pomonella via Sequestration

Gene co-expression network analysis reveals coordinated regulation of three characteristic secondary biosynthetic pathways in tea plant (Camellia sinensis)

Identification and characterization of candidate detoxification genes in Pharsalia antennata Gahan (Coleoptera: Cerambycidae)

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