De novo characterization of transcriptome and gene expression dynamics in epidermis during the larval-pupal metamorphosis of common cutworm

Gu, Jun; Huang, Lixia; Gong, Yan-Jun; Zheng, Sichun; Liu, Lin; Huang, Li-Hua; Feng, Qili

doi:10.1016/j.ibmb.2013.06.001

Cited by 52 publications

(63 citation statements)

References 85 publications

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“…In some studies, chitin content decreased when chitin catabolic enzymes (chitinase and β-N-acetylglucosaminidase) were up-regulated in some holometabolous insects pre-pupae and then increased when chitin synthase was up-regulated in pupae [15]. In the mayfly C .…”

Section: Discussionmentioning

confidence: 99%

“…Next-generation sequencing (NGS) based on the RNA sequencing (RNA-seq) technique has been widely used as a comprehensive approach to study the genome and transcriptome of a broad range of organisms, generating information on many insect transcriptomes including locust ( Locusta migratoria ) [9], field cricket ( Gryllus bimaculatus ) [10], dragonflies ( Enallagma hageni and Ischnura elegans ) [11, 12], mosquito ( Anopheles gambiae ) [13], flour beetle ( Tribolium castaneum ) [14], common cutworm ( Spodoptera litura ) [15], Chinese white wax scale ( Ericerus pela ) [16], and fruit fly ( Drosophila melanogaster ) [17, 18]. However, no transcriptome assembly of mayflies has been published.…”

Section: Introductionmentioning

confidence: 99%

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De novo transcriptome of the mayfly Cloeon viridulum and transcriptional signatures of Prometabola

Qin

Luo

et al. 2017

PLoS ONE

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Mayflies (Ephemeroptera) display many primitive characters and a unique type of metamorphosis (Prometabola). However, information on the genomes and transcriptomes of this insect group is limited. The RNA sequencing study presented here generated the first de novo transcriptome assembly of Cloeon viridulum (Ephemeroptera: Baetidae), and compared gene expression signatures among the young larva (YL), mature larva (ML), subimago (SI), and imago (IM) stages of this mayfly. The transcriptome, based on 88 Gb of sequence data, comprised a set of 81,185 high quality transcripts. The number of differentially expressed genes (DEGs) in YL vs. ML, ML vs. SI, and SI vs. IM, was 4,825, 1,584, and 1,278, respectively, according to the reads per kilobase of transcript per million mapped reads analysis, assuming a false discovery rate <0.05 and a fold change >2. Gene enrichment analysis revealed that these DEGs were enriched in the “chitin metabolic process”, “germ cell development”, “steroid hormone biosynthesis”, and “cutin, suberine, and wax biosynthesis” pathways. Finally, the expression pattern of a selected group of candidate signature genes for Prometabola, including vestigial, methoprene-tolerant, wingless, and broad-complex were confirmed by quantitative real time-PCR analysis. The Q-PCR analysis of larval, subimaginal, and imaginal stages of C. viridulum suggests that the development of mayflies more closely resembles hemimetamorphosis than holometamorphosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

De novo transcriptome of the mayfly Cloeon viridulum and transcriptional signatures of Prometabola

Qin

Luo

et al. 2017

PLoS ONE

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“…The synthesis of chitin relies on two key enzymes, chitin synthase (CHS) and glutamine-fructose-6-phosphate aminotransferase (GFAT), which provides the GlcNAc precursor for the chitin biosynthetic pathway. Insect epidermal metamorphosis is a complicated process involving multiple genes and pathways (Gu et al ., 2013). In our study, the number of unigenes related to chitin degradation was much higher than for chitin biosynthesis and involved a relatively high RPKM value.…”

Section: Discussionmentioning

confidence: 99%

“…During insect growth and development, the cuticle and PM must be degraded periodically and then be replaced in order to allow for growth, maturation and repair (Zakariassen et al ., 2010; Zhang et al ., 2011). One important characteristic of the epidermis is that two closely related processes (the degradation and synthesis of chitin) occur almost simultaneously during metamorphosis (Gu et al ., 2013). Chitin is digested to GlcNAc in the larval cuticle by a binary enzyme system composed of endochitinase and exochitinase.…”

Section: Introductionmentioning

confidence: 99%

“…The synthesis of chitin relies on two key enzymes, chitin synthase (CHS) and glutamine-fructose-6-phosphate aminotransferase (GFAT), which provide the GlcNAc precursor for the chitin biosynthetic pathway (Kramer and Koga, 1986; Kramer et al ., 1995). Although RNAseq-based transcriptome analyses of Spodoptera litura (Lepidoptera) and the Lymantria dispar ( L. Dispar ) larval midgut in response to infection by B. thuringiensis have been undertaken (Gu et al ., 2013), more comprehensive descriptions of the transcriptomes of other (uninfected) lepidopteran species would be beneficial in elucidating chitin-related metabolic processes in these organisms, thereby helping scientists to find potentially new targets for biocontrol.…”

Section: Introductionmentioning

confidence: 99%

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Sequencing and de novo assembly of the Asian gypsy moth transcriptome using the Illumina platform

et al. 2016

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The Asian gypsy moth (Lymantria dispar) is a serious pest of forest and shade trees in many Asian and some European countries. However, there have been few studies of L. dispar genetic information and comprehensive genetic analyses of this species are needed in order to understand its genetic and metabolic sensitivities, such as the molting mechanism during larval development. In this study, high-throughput sequencing technology was used to sequence the transcriptome of the Asian subspecies of the gyspy moth, after which a comprehensive analysis of chitin metabolism was undertaken. We generated 37,750,380 high-quality reads and assembled them into contigs. A total of 37,098 unigenes were identified, of which 15,901 were annotated in the NCBI non-redundant protein database and 9,613 were annotated in the Swiss-Prot database. We mapped 4,329 unigenes onto 317 pathways using the Kyoto Encyclopedia of Genes and Genomes Pathway database. Chitin metabolism unigenes were found in the transcriptome and the data indicated that a variety of enzymes was involved in chitin catabolic and biosynthetic pathways.

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PROTEIN PROFILES OF CHINESE WHITE WAX SCALE, Ericerus pela, AT THE MALE PUPAL STAGE BY HIGH‐THROUGHPUT PROTEOMICS

Yang

Chen

2014

Arch Insect Biochem Physiol

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The Chinese white wax scale insect (Ericerus pela) is sexually dimorphic with holometabolous males and hemimetabolous females. Holometabolous insects were assumed to originate from hemimetabolous ancestors. Therefore, the male pupal stage is a major innovation compared with hemimetabolous female insects. Here, the protein profiles of the male pupae were obtained by high-throughput proteomics and analyzed using bioinformatics methods. A total of 1,437 peptides were identified and assigned to 677 protein groups. Most of the proteins had molecular weights below 40 kDa and isoelectric points from 4 to 7. Gene Ontology terms were assigned to 331 proteins, including metabolic process, developmental process, and cellular process. Kyoto Encyclopedia of Genes and Genomes annotations identified 142 pathways and most proteins were assigned to metabolism events. Pathways involved in cell growth and death, signal transduction, folding, and sorting and degradation were also identified. Six proteins that had undergone positive selection were classified into four groups, protein biosynthesis, protein degeneration, signal transduction, and detoxification. Many of the high-abundance proteins were enzymes involved in carbohydrate, lipid, and amino acid metabolism; signal transduction; degradation; and immunization, which indicated that metabolism, disruption, and development occurred intensely at the pupal stage. These processes are closely related to the physiological status of pupae. The results also suggested that these related proteins may be fundamental factors in the formation of pupae. This study describes pupal characterization at the molecular level and provides a basis for further physiological studies.

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De novo characterization of transcriptome and gene expression dynamics in epidermis during the larval-pupal metamorphosis of common cutworm

Cited by 52 publications

References 85 publications

De novo transcriptome of the mayfly Cloeon viridulum and transcriptional signatures of Prometabola

De novo transcriptome of the mayfly Cloeon viridulum and transcriptional signatures of Prometabola

Sequencing and de novo assembly of the Asian gypsy moth transcriptome using the Illumina platform

PROTEIN PROFILES OF CHINESE WHITE WAX SCALE, Ericerus pela, AT THE MALE PUPAL STAGE BY HIGH‐THROUGHPUT PROTEOMICS

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