de Novo-designed antimicrobial peptides with broad-spectrum antimicrobial potency and rapid wound disinfection

Zheng, Yongfang; Chen, ShiXian; Mao, Kejing; Zhu, XinYu; Jiang, MingYi; Wu, Chang-Jer; Lu, JianRen; Zhu, Hu

doi:10.1016/j.colsurfa.2023.131355

Cited by 3 publications

(4 citation statements)

References 43 publications

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“…The sterilization kinetics assay was performed following previous methods ( Peng et al., 2022 ; Yong Fang et al., 2023 ). Gentamicin, C2-1 and C2-2 at a concentration of 8 μ g/mL each were added to a bacterial suspension of 1×10 5 CFU/mL, and incubated at 37°C for 0, 15, 30, 60, 120, or 240min.…”

Section: Methodsmentioning

confidence: 99%

CATH-2-derived antimicrobial peptide inhibits multidrug-resistant Escherichia coli infection in chickens

Hao,

Shi,

Chen

et al. 2024

Front. Cell. Infect. Microbiol.

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Avian colibacillosis (AC), caused by infection with Escherichia coli (E. coli), is a major threat to poultry health, food safety and public health, and results in high mortality and significant economic losses. Currently, new drugs are urgently needed to replace antibiotics due to the continuous emergence and increasing resistance of multidrug-resistant (MDR) strains of E. coli caused by the irrational use of antibiotics in agriculture and animal husbandry. In recent years, antimicrobial peptides (AMPs), which uniquely evolved to protect the host, have emerged as a leading alternative to antibiotics in clinical settings. CATH-2, a member of the antimicrobial cathelicidin peptide family, has been reported to have antibacterial activity. To enhance the antimicrobial potency and reduce the adverse effects on animals, we designed five novel AMPs, named C2-1, C2-2, C2-3, C2-4 and C2-5, based on chicken CATH-2, the secondary structures of these AMPs were consistently α-helical and had an altered net charge and hydrophobicity compared to those of the CATH-2 (1-15) sequences. Subsequently, the antimicrobial activities of CATH-2 (1-15) and five designed peptides against MDR E. coli were evaluated in vitro. Specifically, C2-2 showed excellent antimicrobial activity against either the ATCC standard strain or veterinary clinical isolates of MDR E. coli, with concentrations ranging from 2-8 μg/mL. Furthermore, C2-2 maintained its strong antibacterial efficacy under high temperature and saline conditions, demonstrating significant stability. Similarly, C2-2 retained a high level of safety with no significant hemolytic activity on chicken mature red blood cells or cytotoxicity on chicken kidney cells over the concentration range of 0-64 μg/mL. Moreover, the administration of C2-2 improved the survival rate and reduced the bacterial load in the heart, liver and spleen during MDR E. coli infection in chickens. Additionally, pathological damage to the heart, liver and intestine was prevented when MDR E. coli infected chickens were treated with C2-2. Together, our study showed that C2-2 may be a promising novel therapeutic agent for the treatment of MDR E. coli infections and AC.

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Section: Methodsmentioning

confidence: 99%

CATH-2-derived antimicrobial peptide inhibits multidrug-resistant Escherichia coli infection in chickens

Hao,

Shi,

Chen

et al. 2024

Front. Cell. Infect. Microbiol.

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“…The novel AMP FWKFK was manufactured using Fmoc chemistry, and the product was tested using modified cell membrane chromatography [ 71 ]. D-GW1, a short AMP, was developed and has been shown to improve wound healing [ 72 ].…”

Section: Reviewmentioning

confidence: 99%

“…In mammalian anti-infection animal models, the antibacterial peptide FWKFK demonstrated strong antibacterial activity, suggesting that it may have use in wound healing [ 71 ]. An in vivo rat open wound model was employed to illustrate the usefulness of D-GW1 in wound healing, as it not only dramatically suppressed bacterial growth but also aided in wound healing and re-epithelialization [ 72 ]. Furthermore, the treatment group's regenerated granulation tissue thicknesses were significantly higher than those of the control group, providing additional evidence for D-GW1's effectiveness in accelerating wound healing [ 72 ].…”

Section: Reviewmentioning

confidence: 99%

“…An in vivo rat open wound model was employed to illustrate the usefulness of D-GW1 in wound healing, as it not only dramatically suppressed bacterial growth but also aided in wound healing and re-epithelialization [ 72 ]. Furthermore, the treatment group's regenerated granulation tissue thicknesses were significantly higher than those of the control group, providing additional evidence for D-GW1's effectiveness in accelerating wound healing [ 72 ]. AMP (RWPIL) has demonstrated promising properties against bacteria that are resistant to drugs on wounds, hence facilitating the healing process [ 62 ].…”

Section: Reviewmentioning

confidence: 99%

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A Systematic Review of the Design and Applications of Antimicrobial Peptides in Wound Healing

Ahmad,

Khan,

Bandy

2024

Cureus

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The sources of antimicrobial peptides (AMPs), also known as peptide-based antibiotics, are diverse, such as plants, animals, microorganisms including human leukocytes, saliva, human defense peptides, and human sweat. These natural sources provide a rich variety of AMPs with unique characteristics and potential therapeutic applications, including wound-healing and antimicrobial properties. AMPs derived from these sources have shown promise in combating a wide range of pathogens, making them valuable targets for further research and potential clinical applications. The design of AMPs for wound healing involves a meticulous process of structurally optimizing peptides to possess a unique combination of antibacterial and wound-healing characteristics. This systematic review was produced to show the design and applications of AMPs in wound healing. The terms "antimicrobial peptides AND wound healing" were used to search for articles published between September 2023 and January 2010. In the search, we found a total of 12958 articles, of which 12898 were excluded, and the remaining 60 articles were chosen for further study. This systematic review underscores the potential of AMPs as valuable tools in infection control and wound healing, showcasing their versatility and effectiveness in combating a wide range of pathogens. Overall, AMPs in wound healing display a diverse mechanism of action, influencing the inflammatory response, encouraging tissue regeneration, and aiding tissue remodeling, along with strong antibacterial activity. Furthermore, this systematic review addresses AMP toxicity studies, which include rigorous in vitro and in vivo examinations to determine potential cytotoxic effects, systemic toxicity, and any adverse responses connected with its usage in wound-healing applications.

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Unveiling the arsenal against antibiotic resistance: Antibacterial peptides as broad-spectrum weapons targeting multidrug-resistant bacteria

Hussaini,

Sulaiman,

Abubakar

et al. 2024

The Microbe

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de Novo-designed antimicrobial peptides with broad-spectrum antimicrobial potency and rapid wound disinfection

Cited by 3 publications

References 43 publications

CATH-2-derived antimicrobial peptide inhibits multidrug-resistant Escherichia coli infection in chickens

CATH-2-derived antimicrobial peptide inhibits multidrug-resistant Escherichia coli infection in chickens

A Systematic Review of the Design and Applications of Antimicrobial Peptides in Wound Healing

Unveiling the arsenal against antibiotic resistance: Antibacterial peptides as broad-spectrum weapons targeting multidrug-resistant bacteria

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