De novo genome and transcriptome resources of the Adzuki bean borer Ostrinia scapulalis (Lepidoptera: Crambidae)

Abstract

We present a draft genome assembly with a de novo prediction and automated functional annotation of coding genes, and a reference transcriptome of the Adzuki bean borer, Ostrinia scapulalis, based on RNA sequencing of various tissues and developmental stages. The genome assembly spans 419 Mb, has a GC content of 37.4% and includes 26,120 predicted coding genes. The reference transcriptome holds 33,080 unigenes and contains a high proportion of a set of genes conserved in eukaryotes and arthropods, used as quality assessment of the reconstructed transcripts. The new genomic and transcriptomic data presented here significantly enrich the public sequence databases for the Crambidae and Lepidoptera, and represent useful resources for future researches related to the evolution and the adaptation of phytophagous moths. The genome and transcriptome assemblies have been deposited and made accessible via a NCBI BioProject (id PRJNA390510) and the LepidoDB database (http://bipaa.genouest.org/sp/ostrinia_scapulalis/).

New WGS data and annotation of the heterosomal vs. autosomal localization of Ostrinia scapulalis (Lepidoptera, Crambidae) nuclear genomic scaffolds

“…This article enriches and updates the annotation of Ostrinia scapulalis (Lepidoptera) nuclear genome recently published by Gschloessl et al [1] with an accurate annotation of the chromosomal localization of the scaffolds constituting the nuclear genome assembly.…”

“…Value of the data This article enriches and updates the annotation of Ostrinia scapulalis (Lepidoptera) nuclear genome recently published by Gschloessl et al [1] with an accurate annotation of the chromosomal localization of the scaffolds constituting the nuclear genome assembly. The new genomic data acquired here (whole-genome shotgun sequencing of four libraries, two males and two females) will enrich the public sequence database for this species.…”

“…The dataset described here is composed of new whole-genome sequencing (WGS) data (paired-end sequencing of four libraries with an Illumina HiSeq2500 sequencing technology) and a new annotation of autosomal and heterosomal scaffolds of the nuclear genome of the moth species Ostrinia scapulalis. These new data are complementary to the scaffold-level nuclear genome (hereafter OSCA ) recently assembled for this species [1] . Specifically, we applied the AD-ratio method originally developed by Bidon et al [2] to compare sequencing depth between male and female libraries, and we introduce an accurate labelling (autosomal vs .…”

“… WGS sequencing depth analysis is a promising method to retrieve the autosomal or heterosomal localization of assembly fragments (scaffolds or contigs) obtained through de novo assembly. The annotation data released here provide key information about the autosomal vs. Z-heterosomal localization of scaffolds described in Gschloessl et al [1] . Such annotation is of great value for future evolutionary studies, as genome-wide population genomics analyses (e.g.…”

“…The annotation data released here provide key information about the autosomal vs. Z-heterosomal localization of scaffolds described in Gschloessl et al [1] .…”