De novo lipogenesis in the liver and adipose tissues of ducks during early growth stages after hatching

Ding, Fang; Pan, Zhenxiao; Kou, Jie; Li, Le; Xia, Lu; Hu, Shiyan; Liu, Hehe; Wang, Jiwen

doi:10.1016/j.cbpb.2012.05.014

Cited by 15 publications

(10 citation statements)

References 37 publications

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“…During this time specific adipogenic genes are expressed and triacylglycerol (TG) accumulates and is stored in the cells. However, in poultry due to the low capacity of adipose tissue to perform de novo lipogenesis (DNL) (Ding et al 2012), the addition of oleic acid is needed to supplement the hormonal cocktail to induce preadipocyte differentiation in vitro (Matsubara et al 2008;Wang et al 2011;He et al 2012). The role of oleic acid in promoting adipogenesis is unclear but research conducted by many investigators has provided compelling evidence that oleic acid can significantly activate adipocyterelated genes.…”

Section: Introductionmentioning

confidence: 99%

“…Peking duck (Anas platyrhynchos) is a world famous waterfowl breed that is able to accumulate large quantities of lipid in the subcutaneous adipose tissue (Attia et al 2012;Ding et al 2012). It has been well established that the development of duck adipose tissue is due to a combination of adipocyte hyperplasia and hypertrophy from hatching until 4 weeks of age, after which adipose expansion is accomplished exclusively by adipocyte hypertrophy (Kou et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Isolation, culture and differentiation of duck (Anas platyrhynchos) preadipocytes

Ding

et al. 2014

Cytotechnology

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In the present study, we isolated preadipocytes from the adipose tissue of Peking duck and subsequently cultured them in vitro. Cell counting kit-8 assay was employed to establish the growth curve of duck primary preadipocytes. Meanwhile, after the cells reaching full confluency, they were induced to differentiate into mature adipocytes by the addition of a cocktail containing dexamethasone, insulin, 3-isobutyl-1-methylxanthine, and oleic acid for 8 days. Successful differentiation was demonstrated by the development of lipid droplets and the expression of key marker genes including peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer binding protein-α (CEBP/α) and adipocyte fatty acid-binding protein (FABP4). Our results showed that duck primary preadipocytes began to adhere 12 h after seeding as short spindle shapes or litter triangles, which grew quickly 3 days post attachment and maintained stable after day 7. After 8 days the preadipocytes were induced to differentiate into mature adipocytes, which were stained red by oil red O. Additionally, it showed that during preadipocyte differentiation PPARγ mRNA was highly expressed at day 3, while CEBP/α and FABP4 mRNA peaked at day 5 and 8, respectively. These results indicate that we have successfully isolated and cultured Peking duck preadipocytes and successfully induced them to differentiate into mature adipocytes. This work could lay a foundation for further research into waterfowl adipogenesis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation, culture and differentiation of duck (Anas platyrhynchos) preadipocytes

Ding

et al. 2014

Cytotechnology

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“…Most of the endogenous body lipids are of hepatic origin, and the growth and subsequent fattening of adipose tissue depend more on the availability of plasma triglycerides (TG; O'Hea and Leveille 1969; Alvarenga et al 2011). Our initial data in ducks also demonstrated that DNL mainly occurred in the liver instead of in adipose tissue (Ding et al 2012).…”

Section: Introductionmentioning

confidence: 61%

“…In ducks, our initial data demonstrated that the main DNL site is always the liver in post-hatching ducks and that the adipose tissues are of little importance for DNL (Ding et al 2012). It was therefore concluded that hepatic DNL as an endogenous pathway for lipid synthesis may play an important role in the individual fat mass differences.…”

Section: Discussionmentioning

confidence: 93%

The contributions of hepatic de novo lipogenesis to the difference in body fat mass of genetically lean and fat duck breeds

Liu

Zhou

et al. 2017

Journal of Applied Animal Research

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The underlying reasons for genetic differences in body fat mass remain unclear. The objective of this study was to investigate the contributions of hepatic de novo lipogenesis (DNL) to the genetic differences in fat mass in ducks. Ducks with distinct genetic backgrounds were selected for the genetically lean and fat animal models. The weights of fat tissue and organs as well as the gene expressions, enzyme activities and concentrations related to hepatic lipogenesis were measured and compared between the two genetically different duck breeds. Although a clear phenotypic difference in extra-liver fat mass between the two genetically different duck breeds was observed, the relative weights of the fat tissue to body weight in the two breeds were similar. There were no clear divergences regarding DNL-related gene expressions (Acc, Dgat2 and Fas), enzyme activities (ELVOL and FAS) and enzyme concentrations (ACC) between the two genetically different duck breeds, suggesting that hepatic DNL may contribute less to the individual fat mass difference in ducks; rather, body fat mass may depend more on the liver size. Our findings may provide a fundamental explanation for individual fat mass differences. ARTICLE HISTORY

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“…These genes are involved in the de novo lipogenesis pathway (38,39) which reflects the ability to store carbohydrate sources as lipids (40). In a context of nutritional change with a sudden high intake of carbohydrates, it is consistent to stimulate their storage by increasing the expression of genes involved in lipid synthesis, the liver being the predominant site of lipogenesis in birds (41,42).…”

Section: Discussionmentioning

confidence: 99%

Ontogeny of hepatic metabolism in mule ducks highlights different gene expression profiles between carbohydrate and lipid metabolic pathways

Massimino

Davail

Seccula

et al. 2020

Preprint

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Background : The production of foie gras involves different metabolic pathways in the liver of overfed ducks such as lipid synthesis and carbohydrates catabolism, but the establishment of these pathways has not yet been described with precision during embryogenesis. The early environment can have short- and long-term impacts on the physiology of many animal species and can be used to influence physiological responses that is called programming. This study proposes to describe the basal hepatic metabolism at the level of mRNA in mule duck embryos in order to reveal potential interesting programming windows in the context of foie gras production. To this end, a kinetic study was designed to determine the level of expression of selected genes involved in steatosis-related liver functions throughout embryogenesis. The livers of 20 mule duck embryos were collected every four days from the 12 th day of embryogenesis (E12) until 4 days after hatching (D4), and gene expression analysis was performed. The expression levels of 50 mRNAs were quantified for these 7 sampling points and classified into 4 major cellular pathways. Results : Interestingly, most mRNAs involved in lipid metabolism are overexpressed after hatching (FASN, SCD1, ACOX1), whereas genes implicated in carbohydrate metabolism (HK1, GAPDH, GLUT1) and development (HGF, IGF, FGFR2) are predominantly overexpressed from E12 to E20. Finally, regarding cellular stress, gene expression appears quite stable throughout development, contrasting with strong expression after hatching (CYP2E1, HSBP1, HSP90AA1). Conclusion : For the first time we described the kinetics of hepatic ontogenesis at mRNA level in mule ducks and highlighted different expression patterns depending on the cellular pathway. These results could be particularly useful in the design of embryonic programming for the production of foie gras.

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De novo lipogenesis in the liver and adipose tissues of ducks during early growth stages after hatching

Cited by 15 publications

References 37 publications

Isolation, culture and differentiation of duck (Anas platyrhynchos) preadipocytes

Isolation, culture and differentiation of duck (Anas platyrhynchos) preadipocytes

The contributions of hepatic de novo lipogenesis to the difference in body fat mass of genetically lean and fat duck breeds

Ontogeny of hepatic metabolism in mule ducks highlights different gene expression profiles between carbohydrate and lipid metabolic pathways

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