De novo peptides as potential antimicrobial agents

Amarh, Margaret Amerley; Laryea, Michael Konney; Borquaye, Lawrence Sheringham

doi:10.1016/j.heliyon.2023.e19641

Cited by 4 publications

(1 citation statement)

References 52 publications

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“…For instance, antithrombin acts as an anticoagulant, reducing clot formation and thrombotic risks [ 35 , 36 ]. Antimicrobial peptides enhance innate immunity against bacterial pathogens [ 37 , 38 , 39 ], while ACE-inhibitory peptides regulate blood pressure [ 17 , 40 , 41 ]. Immunomodulatory peptides manage immune reactions and inflammation, bolstering overall resilience [ 17 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Heat Treatment on Protein Self-Digestion in Ruminants’ Milk

Leite,

Montoya,

Maes

et al. 2023

Foods

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This study investigated whether heat treatments (raw, 63 °C for 30 min, and 85 °C for 5 min) affect protein hydrolysis by endogenous enzymes in the milk of ruminants (bovine, ovine, and caprine) using a self-digestion model. Self-digestion consisted of the incubation for six hours at 37 °C of the ruminants’ milk. Free amino group concentration was measured by the o-phthaldialdehyde method, and peptide sequences were identified by chromatography-mass spectrometry. Results showed that heat treatments prior to self-digestion decreased the free NH2 by 59% in bovine milk heated at 85 °C/5 min, and by 44 and 53% in caprine milk heated at 63 °C/30 min and 85 °C/5 min, respectively. However, after self-digestion, only new free amino groups were observed for the raw and heated at 63 °C/30 min milk. β-Casein was the most cleaved protein in the raw and heated at 63 °C/30 min bovine milk. A similar trend was observed in raw ovine and caprine milk. Self-digestion increased 6.8-fold the potential antithrombin peptides in the bovine milk heated at 63 °C/30 min. Enhancing bioactive peptide abundance through self-digestion has potential applications in the industry for functional products. Overall, heat treatments affected the free amino groups according to the species and heat treatment applied, which was reflected in the varying degrees of cleaved peptide bonds and peptides released during self-digestion.

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Section: Discussionmentioning

confidence: 99%

Effect of Heat Treatment on Protein Self-Digestion in Ruminants’ Milk

Leite,

Montoya,

Maes

et al. 2023

Foods

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Empowering Antimicrobial Peptides: Harnessing Nanotechnology and Engineering Strategies to Combat Microbial Resistance

Avinash,

Bisht,

Kumar

et al. 2024

Peptide Science

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Antimicrobial peptides (AMPs) are being studied for their potential in addressing microbial resistance, a significant issue in treating infections. More than 22,500 AMPs are listed in the database, but their clinical use faces challenges like instability and selectivity. Nanodrug delivery systems are now seen as a beneficial method to improve the effectiveness of AMPs. These systems can shield AMPs from degradation, enhance their availability, and deliver them to specific infection sites, enhancing their ability to combat resistance. One example is liposomal nanoparticles loaded with a mix of AMPs, targeting drug‐resistant bacteria directly. Strategies to develop novel AMPs through engineering methods, including design approaches, computational techniques, and modifications for stability, are discussed in this review. Combinatorial approaches with antibiotics, patents, and clinical trials involving AMPs against infections are also considered, highlighting the potential of AMPs in battling microbial resistance.

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De Novo Design and Characterization of Novel Antimicrobial Peptides, Lk3 and Lk4, Containing Repeating Unit

Koshki,

Farrokh

2024

Int J Pept Res Ther

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De novo peptides as potential antimicrobial agents

Cited by 4 publications

References 52 publications

Effect of Heat Treatment on Protein Self-Digestion in Ruminants’ Milk

Effect of Heat Treatment on Protein Self-Digestion in Ruminants’ Milk

Empowering Antimicrobial Peptides: Harnessing Nanotechnology and Engineering Strategies to Combat Microbial Resistance

De Novo Design and Characterization of Novel Antimicrobial Peptides, Lk3 and Lk4, Containing Repeating Unit

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