De novo reconstitution of a functional mammalian urinary bladder by tissue engineering

Oberpenning, F.; Meng, Jiao; Yoo, James J.; Atala, Anthony

doi:10.1038/6146

Cited by 731 publications

(248 citation statements)

References 31 publications

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“…Organization of transplanted cells to form growth-plate-like structures has not been reported, but other cell types (30)(31)(32)(33)(34)(35)(36) have demonstrated the ability to selfassemble in vitro into structures that have similar functional and͞or morphological properties to the tissues from which they were isolated (37). In addition, several groups have engineered complex functional tissues such as the urinary bladder (38) and the small intestine (39) through the transplantation of multiple cell types in specific locations on the delivery vehicle. However, the development of growth-plate-like structures presented here is a striking demonstration of the ability of randomly mixed multiple cell types to self-organize into several distinct tissue types.…”

Section: Resultsmentioning

confidence: 99%

Engineering growing tissues

Alsberg

Anderson

Albeiruti

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

366

224

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Regenerating or engineering new tissues and organs may one day allow routine replacement of lost or failing tissues and organs. However, these engineered tissues must not only grow to fill a defect and integrate with the host tissue, but often they must also grow in concert with the changing needs of the body over time. We hypothesized that tissues capable of growing with time could be engineered by supplying growth stimulus signals to cells from the biomaterial used for cell transplantation. In this study, chondrocytes and osteoblasts were cotransplanted on hydrogels modified with an RGD-containing peptide sequence to promote cell multiplication. New bone tissue was formed that grew in mass and cellularity by endochondral ossification in a manner similar to normal long-bone growth. Transplanted cells organized into structures that morphologically and functionally resembled growth plates. These engineered tissues could find utility in treating diseases and injuries of the growth plate, testing the effect of experimental drugs on growth-plate function and development, and investigating the biology of long-bone growth. Furthermore, this concept of promoting the growth of engineered tissues could find great utility in engineering numerous tissue types by way of the transplantation of a small number of precursor cells.bone ͉ cartilage ͉ alginate ͉ adhesion ligands ͉ endochondral ossification

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Section: Resultsmentioning

confidence: 99%

Engineering growing tissues

Alsberg

Anderson

Albeiruti

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

366

224

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“…We used primary cells harvested from canine bladders using established protocols (10,(37)(38)(39). Bladder tissue was microdissected, and the mucosal and muscular layers were scalpel under sterile conditions.…”

Section: Cell Cultivation and Seedingmentioning

confidence: 99%

“…Traditionally, two main classes of biomaterials have been utilized for the engineering of hollow organs; acellular matrices derived from donor tissues (3)(4)(5)(6)(7)(8), (e.g., bladder submucosa (lamina propria) and small intestinal submucosa), and synthetic polymers such as polyglycolic acid (PGA) (9,10), polylactic acid (PLA), and poly(lactic-co-glycolic acid) (PLGA). These materials have been tested in respect to their biocompatibility in the host tissues (11,12).…”

Section: Introductionmentioning

confidence: 99%

Composite scaffolds for the engineering of hollow organs and tissues

Eberli¹,

Filho²,

Atala³

et al. 2009

Methods

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Composite scaffolds for the engineering of hollow organs and tissues AbstractSeveral types of synthetic and naturally derived biomaterials have been used for augmenting hollow organs and tissues. However, each has desirable traits which were exclusive of the other. We fabricated a composite scaffold and tested its potential for the engineering of hollow organs in a bladder tissue model. The composite scaffolds were configured to accommodate a large number of cells on one side and were designed to serve as a barrier on the opposite side. The scaffolds were fabricated by bonding a collagen matrix to PGA polymers with threaded collagen fiber stitches. Urothelial and bladder smooth muscle cells were seeded on the composite scaffolds, and implanted in mice for up to 4 weeks and analyzed. Both cell types readily attached and proliferated on the scaffolds and formed bladder tissue-like structures in vivo. These structures consisted of a luminal urothelial layer, a collagen rich compartment and a peripheral smooth muscle layer. Biomechanical studies demonstrated that the tissues were readily elastic while maintaining their pre-configured structures. This study demonstrates that a composite scaffold can be fabricated with two completely different polymer systems for the engineering of hollow organs. The composite scaffolds are biocompatible, possess adequate physical and structural characteristics for bladder tissue engineering, and are able to form tissues in vivo. This scaffold system may be useful in patients requiring hollow organ replacement. Title: COMPOSITE SCAFFOLDS FOR THE ENGINEERING OF HOLLOW ORGANS AND TISSUES Authors Abstract:Several types of synthetic and naturally derived biomaterials have been used for augmenting hollow organs and tissues. However, each has desirable traits which were exclusive of the other. We fabricated a composite scaffold and tested its potential for the engineering of hollow organs in a bladder tissue model. The composite scaffolds were configured to accommodate a large number of cells on one side and were designed to serve as a barrier on the opposite side. The scaffolds were fabricated by bonding a collagen matrix to PGA polymers with threaded collagen fiber stitches. Urothelial and bladder smooth muscle cells were seeded on the composite scaffolds, and implanted in mice for up to 4 weeks and analyzed. Both cell types readily attached and proliferated on the scaffolds and formed bladder tissue-like structures in vivo. These structures consisted of a luminal urothelial layer, a collagen rich compartment and a peripheral smooth muscle layer. Biomechanical studies demonstrated that the tissues were readily elastic while maintaining their pre-configured structures. This study demonstrates that a composite scaffold can be fabricated with two completely different polymer systems for the engineering of hollow organs. The composite scaffolds are biocompatible, possess adequate physical and structural characteristics for bladder tissue engineering, and are able to form tissues in vivo. Thi...

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“…However, if the defects are large, fibrosis and scar formation ensue. 7,8 In a similar manner, vaginal organs were engineered by using artificially created polyglycolic-acid matrices either alone or seeded with vaginal muscle and epithelial cells. The biomaterial scaffoldonly implants fibrosed and constricted over time.…”

Section: Principles Of Wound Healing and Tissue Regenerationmentioning

confidence: 99%

Wound Healing Versus Regeneration: Role of the Tissue Environment in Regenerative Medicine

Atala¹,

Irvine²,

Moses³

et al. 2010

MRS Bull.

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One of the major challenges in the field of regenerative medicine is how to optimize tissue regeneration in the body by therapeutically manipulating its natural ability to form scar at the time of injury or disease. It is often the balance between tissue regeneration, a process that is activated at the onset of disease, and scar formation, which develops as a result of the disease process that determines the ability of the tissue or organ to be functional. Using biomaterials as scaffolds often can provide a “bridge” for normal tissue edges to regenerate over small distances, usually up to 1 cm. Larger tissue defect gaps typically require both scaffolds and cells for normal tissue regeneration to occur without scar formation. Various strategies can help to modulate the scar response and can potentially enhance tissue regeneration. Understanding the mechanistic basis of such multivariate interactions as the scar microenvironment, the immune system, extracellular matrix, and inflammatory cytokines may enable the design of tissue engineering and wound healing strategies that directly modulate the healing response in a manner favorable to regeneration.

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De novo reconstitution of a functional mammalian urinary bladder by tissue engineering

Cited by 731 publications

References 31 publications

Engineering growing tissues

Engineering growing tissues

Composite scaffolds for the engineering of hollow organs and tissues

Wound Healing Versus Regeneration: Role of the Tissue Environment in Regenerative Medicine

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