De Novo Regeneration in Chickpea Genotypes (Cicer Arietinum L.)

Abstract

Direct regeneration from mature embryo axes was achieved without intervening of callus phase in four chickpea varieties on the Media MS and B5 supplemented with combination of BAP, NAA and Kinetin. Hundred percent regeneration capacity was exhibited by commercially grown Vijay and Vishal varieties. There was considerable variation in umber of multiple shoot production by different varieties. Profuse rooting was obtained on the medium containing 0.5 and 1.0 mg/l IBA. This protocol is optimized for complete plant regeneration of chickpea for genetic transformation.