De novo Sequencing, Assembly and Characterization of Antennal Transcriptome of Anomala corpulenta Motschulsky (Coleoptera: Rutelidae)

Abstract

Background Anomala corpulenta is an important insect pest and can cause enormous economic losses in agriculture, horticulture and forestry. It is widely distributed in China, and both larvae and adults can cause serious damage. It is difficult to control this pest because the larvae live underground. Any new control strategy should exploit alternatives to heavily and frequently used chemical insecticides. However, little genetic research has been carried out on A. corpulenta due to the lack of genomic resources. Genomic resources could be produced by next generation sequencing technologies with low cost and in a short time. In this study, we performed de novo sequencing, assembly and characterization of the antennal transcriptome of A. corpulenta.ResultsIllumina sequencing technology was used to sequence the antennal transcriptome of A. corpulenta. Approximately 76.7 million total raw reads and about 68.9 million total clean reads were obtained, and then 35,656 unigenes were assembled. Of these unigenes, 21,463 of them could be annotated in the NCBI nr database, and, among the annotated unigenes, 11,154 and 6,625 unigenes could be assigned to GO and COG, respectively. Additionally, 16,350 unigenes could be annotated in the Swiss-Prot database, and 14,499 unigenes could map onto 258 pathways in the KEGG Pathway database. We also found 24 unigenes related to OBPs, 6 to CSPs, and in total 167 unigenes related to chemodetection. We analyzed 4 OBPs and 3CSPs sequences and their RT-qPCR results agreed well with their FPKM values.ConclusionWe produced the first large-scale antennal transcriptome of A. corpulenta, which is a species that has little genomic information in public databases. The identified chemodetection unigenes can promote the molecular mechanistic study of behavior in A. corpulenta. These findings provide a general sequence resource for molecular genetics research on A. corpulenta.

Antennal Transcriptome Analysis of Odorant Reception Genes in the Red Turpentine Beetle (RTB), Dendroctonus valens

“…The data were obtained from the current study for D . valens and from references [ 7 , 20 , 24 , 91 ] for Drosophila melanogaster , [ 92 ] for Sesamia inferens ,[ 20 , 24 , 62 , 93 – 95 ] for Bomby mori , [ 7 , 20 , 24 , 91 ] for Tribolium castaneum ,[ 5 ] for I . typographus and D .…”

De novo sequencing and characterization of the Bradysia odoriphaga (Diptera: Sciaridae) larval transcriptome

“…Recently, Illumina RNAseqs have been used for efficient assembly of de novo transcriptomes for many insects, such as Bemisia tabaci (Wang et al, 2010), Anopheles funestus (Crawford et al, 2010), and Locusta migratoria (Chen et al, 2010). Transcriptomic information is also being used in gene discovery and SSR mining (Wang et al, 2010;Li et al, 2012;Zhang et al, 2012;Chen et al, 2014). These studies indicated that Illumina deep sequencing technology can produce exceptional coverage of expressed sequences.…”

Peer Review #2 of "Sex- and tissue-specific expression of odorant-binding proteins and chemosensory proteins in adults of the scarab beetle Hylamorpha elegans (Burmeister) (Coleoptera: Scarabaeidae) (v0.1)"

“…In H. elegans, most of the OBPs were classified as Classic OBPs, which have been found in all insect species studied (Sánchez-Gracia, Vieira & Rozas, 2009). Unlike T. castaneum, (Abdel-Latief, 2007) A. corpulenta (Chen et al, 2014) and D. ponderosae (Andersson et al, 2013) H. elegans did not show Minus-C OBPs.…”