De novo shoot organogenesis: from art to science

Duclercq, Jérôme; Sangwan‐Norreel, B. S.; Catterou, Manuella; Sangwan, Rajbir S.

doi:10.1016/j.tplants.2011.08.004

Cited by 181 publications

(149 citation statements)

References 89 publications

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“…Pioneer studies demonstrated that cooperation of exogenous cytokinin and auxin induces plant regeneration, which lays an important foundation for wide applications in plant biotechnology and agricultural practices (Duclercq et al, 2011;Ikeuchi et al, 2016). However, the mechanisms underlying this regeneration process are poorly understood.…”

Section: A Proposed Regulatory Network For De Novo Specification Of Smentioning

confidence: 99%

“…Six decades ago, Skoog and Miller found that under the induction of exogenous cytokinin and auxin, adventitious shoots could be induced in vitro from a mass of proliferating cells named callus (Skoog and Miller, 1957;Sugimoto et al, 2010;Iwase et al, 2011). This process is defined as de novo shoot regeneration and provides an ideal system for studying the specification of stem cell niche in plants (Duclercq et al, 2011;Iwase et al, 2017). During shoot regeneration, WUS expression promotes cell fate transition from callus cells to OC, which is essential for the specification of the shoot stem cell niche and the subsequent establishment of the shoot meristem (Duclercq et al, 2011;Aichinger et al, 2012;Ikeuchi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…This process is defined as de novo shoot regeneration and provides an ideal system for studying the specification of stem cell niche in plants (Duclercq et al, 2011;Iwase et al, 2017). During shoot regeneration, WUS expression promotes cell fate transition from callus cells to OC, which is essential for the specification of the shoot stem cell niche and the subsequent establishment of the shoot meristem (Duclercq et al, 2011;Aichinger et al, 2012;Ikeuchi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Type-B ARABIDOPSIS RESPONSE REGULATORs Specify the Shoot Stem Cell Niche by Dual Regulation of WUSCHEL

et al. 2017

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ORCID IDs: 0000-0003-3103-8629 (W.J.M.); 0000-0002-3129-5206 (X.S.Z.)Plants are known for their capacity to regenerate the whole body through de novo formation of apical meristems from a mass of proliferating cells named callus. Exogenous cytokinin and auxin determine cell fate for the establishment of the stem cell niche, which is the vital step of shoot regeneration, but the underlying mechanisms remain unclear. Here, we show that type-B ARABIDOPSIS RESPONSE REGULATORs (ARRs), critical components of cytokinin signaling, activate the transcription of WUSCHEL (WUS), which encodes a key regulator for maintaining stem cells. In parallel, type-B ARRs inhibit auxin accumulation by repressing the expression of YUCCAs, which encode a key enzyme for auxin biosynthesis, indirectly promoting WUS induction. Both pathways are essential for de novo regeneration of the shoot stem cell niche. In addition, the dual regulation of type-B ARRs on WUS transcription is required for the maintenance of the shoot apical meristem in planta. Thus, our results reveal a long-standing missing link between cytokinin signaling and WUS regulator, and the findings provide critical information for understanding cell fate specification.

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Section: A Proposed Regulatory Network For De Novo Specification Of Smentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Type-B ARABIDOPSIS RESPONSE REGULATORs Specify the Shoot Stem Cell Niche by Dual Regulation of WUSCHEL

et al. 2017

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“…De novo organogenesis, through which detached or wounded plant organs regenerate adventitious roots and shoots, commonly occurs under natural conditions and allows plants to recover from damage (Duclercq et al, 2011;Xu and Huang, 2014). The ability of plants to undergo de novo organogenesis is also widely exploited in agricultural biotechnologies such as tissue culture and vegetative reproduction via cuttings (Sussex, 2008).…”

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confidence: 99%

Auxin-Independent NAC Pathway Acts in Response to Explant-Specific Wounding and Promotes Root Tip Emergence during de Novo Root Organogenesis in Arabidopsis

et al. 2016

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Plants have powerful regenerative abilities that allow them to recover from damage and survive in nature. De novo organogenesis is one type of plant regeneration in which adventitious roots and shoots are produced from wounded and detached organs. By studying de novo root organogenesis using leaf explants of Arabidopsis (Arabidopsis thaliana), we previously suggested that wounding is the first event that provides signals to trigger the whole regenerative process. However, our knowledge of the role of wounding in regeneration remains limited. In this study, we show that wounding not only triggers the auxin-mediated fate transition of regeneration-competent cells, but also induces the NAC pathway for root tip emergence. The NAC1 transcription factor gene was specifically expressed in response to wounding in the leaf explant, but not in the wounded leaf residue of the source plant. Inhibition of the NAC1 pathway severely affected the emergence of adventitious root tips. However, the NAC1 pathway functioned independently of auxin-mediated cell fate transition and regulates expression of CEP genes, which encode proteins that might have a role in degradation of extensin proteins in the cell wall. Overall, our results suggest that wounding has multiple roles in de novo root organogenesis and that NAC1 acts as one downstream branch in regulating the cellular environment for organ emergence.

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“…Mutations as well as natural genetic variation have been used to trace critical molecular pathways, but despite recent advances, the molecular basis of regeneration is still largely unclear and therefore, many important crop plants have remained recalcitrant to regeneration. 2 Overproduction of cytokinins or overexpression of transcription factors expressed preferentially in developing embryos or specifying embryonic stem cell identity genes have been used to confer unusual regeneration properties in several species. 3 Recently, components of the auxin signaling pathway have also been implicated in increasing regeneration capacities.…”

mentioning

confidence: 99%

Overcoming recalcitrance - Auxin response factor functions in plant regeneration

Ckurshumova

Berleth²

2015

Plant Signaling & Behavior

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De novo shoot organogenesis: from art to science

Cited by 181 publications

References 89 publications

Type-B ARABIDOPSIS RESPONSE REGULATORs Specify the Shoot Stem Cell Niche by Dual Regulation of WUSCHEL

Type-B ARABIDOPSIS RESPONSE REGULATORs Specify the Shoot Stem Cell Niche by Dual Regulation of WUSCHEL

Auxin-Independent NAC Pathway Acts in Response to Explant-Specific Wounding and Promotes Root Tip Emergence during de Novo Root Organogenesis in Arabidopsis

Overcoming recalcitrance - Auxin response factor functions in plant regeneration

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