VIth International Congress on Thrombosis and Haemostasis 1977
DOI: 10.1055/s-0039-1680422
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De Novo Synthesis of Purine Nucleotides in Human Peripheral Blood Platelets

Abstract: Human blood platelets were gtudied for the presence of the pathway of de novo synthesis of purine nucleotides. 8 x 108 cells were incubated for 2.5 h at 37°C in 2 ml of Eagle’s Minimal Essential Medium containing Earle’s Balanced Salt Solution, 15% fetal calf serum and 20 μCi sodium [14C] formate (59 mCi/mmole). Platelets were found to incorporate 14C into total purines at a slow but detectable rate of 50–70 pmoles/8 x 108 cells/2.5 h. This incorporation was inhibited by approximately 80% at 10 mM azaserine an… Show more

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“…Interestingly, in a preliminary experiment we performed, the mRNA levels of PRPS and PRPPAT in the kidneys of goslings infected with GAstV were significantly upregulated compared with the control group (data not published). Purine nucleotides are purine rings that are gradually synthesized with phosphoribosyl molecules [105]. IMPs are synthesized first and then transformed into AMPs and GMPs [105].…”
Section: Uric Acid Metabolism In Poultrymentioning
confidence: 99%
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“…Interestingly, in a preliminary experiment we performed, the mRNA levels of PRPS and PRPPAT in the kidneys of goslings infected with GAstV were significantly upregulated compared with the control group (data not published). Purine nucleotides are purine rings that are gradually synthesized with phosphoribosyl molecules [105]. IMPs are synthesized first and then transformed into AMPs and GMPs [105].…”
Section: Uric Acid Metabolism In Poultrymentioning
confidence: 99%
“…Purine nucleotides are purine rings that are gradually synthesized with phosphoribosyl molecules [105]. IMPs are synthesized first and then transformed into AMPs and GMPs [105]. De novo synthesis of purine nucleotides is the main source of nucleotides in vivo.…”
Section: Uric Acid Metabolism In Poultrymentioning
confidence: 99%