2022
DOI: 10.1007/s11033-022-07784-5
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De novo transcriptome analysis of Justicia adhatoda reveals candidate genes involved in major biosynthetic pathway

Abstract: Background Justicia adhatoda is an important medicinal plant traditionally used in the Indian system of medicine and the absence of molecular-level studies in this plant hinders its wide use, hence the study was aimed to analyse the genes involved in its various pathways. Methods and results The RNA isolated was subjected to Illumina sequencing. De novo assembly was performed using TRINITY software which produced 171,064 transcr… Show more

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Cited by 5 publications
(2 citation statements)
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“…Molecular markers reveal the genetic relationship between the species as they are unaffected by the environment and have easy detection and high heritability [ 67 , 68 ]. These markers are widely used in plant improvement and genetic conservation [ 69 ]. A total of 36,197 SSRs were identified with highest number of mononucleotide motifs compared to di-, tri-, tetra-, penta-, and hexa-nucleotide motifs.…”
Section: Discussionmentioning
confidence: 99%
“…Molecular markers reveal the genetic relationship between the species as they are unaffected by the environment and have easy detection and high heritability [ 67 , 68 ]. These markers are widely used in plant improvement and genetic conservation [ 69 ]. A total of 36,197 SSRs were identified with highest number of mononucleotide motifs compared to di-, tri-, tetra-, penta-, and hexa-nucleotide motifs.…”
Section: Discussionmentioning
confidence: 99%
“…The indole pathway begins with chorismite that is catalyzed by anthranilate synthase (AS) to form anthranilate, followed by various enzymic reactions (six steps) to give tryptamine. Finally, strictosidin synthase (STR) catalyzes the formation of strictosidine from secologanin and tryptamine [31][32][33][34]. Gelsemium alkaloids are then obtained.…”
Section: Introductionmentioning
confidence: 99%