Deamination of protonated amines to yield protonated imines

Zhang, Duxi; Gill, Lynn A.; Cooks, R. Graham

doi:10.1016/s1044-0305(98)00093-2

Cited by 20 publications

(19 citation statements)

References 31 publications

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“…Deconvolution of averaged ESI-MS spectra was automatically performed by using Bioworks Browser software provided with the Deca XP instrument or MagTran 1.0 software [25]. Experimental mass values of statherin, P-B peptide and their derivatives were compared with average theoretical mass values using PeptideMass and FindPept programs, available at the Swiss-Prot Data Bank (us.expasy.org/tools), where statherin and P-B peptide are coded as P02808 and P02814, respectively.…”

Section: Discussionmentioning

confidence: 99%

Detection in human saliva of different statherin and P-B fragments and derivatives

et al. 2006

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Statherin is a multifunctional polypeptide specific of human saliva involved in oral calcium homeostasis, phosphate buffering and formation of protein networks. Salivary P-B peptide is usually included into the basic proline-rich protein family but it shows some similarities with statherin and its specific biological role is still undefined. In this study, various fragments and derivatives of statherin and P-B peptide were consistently detected by RP-HPLC ESI-IT MS in 23 samples of human saliva. They were: statherin mono- and non-phosphorylated, statherin Des-Phe(43) (statherin SV1), statherin Des-Thr(42),Phe(43), statherin Des-Asp(1), statherin Des(6-15) (statherin SV2), statherin Des(1-9), statherin Des(1-10), statherin Des(1-13) and P-B Des(1-5). Statherin SV3 (statherin Des(6-15), Phe(43)) was detected only in one sample. Identity of the fragments was confirmed either by MS/MS experiments or by enzymatic digestion or by Edman sequencing. Detection of the fragments suggests that statherin and P-B peptide are submitted to post-translational proteolytic cleavages that are common to other classes of salivary proteins.

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Section: Discussionmentioning

confidence: 99%

Detection in human saliva of different statherin and P-B fragments and derivatives

et al. 2006

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“…This pathway requires proton transfer to the hydroxyl group of the alcohol followed by nucleophilic attack of the nitrile nitrogen on the a-carbon of the protonated alcohol. Zhang, Gill, & Cooks (1998) have investigated related reactions of protonated amines with amines in a quadrupole ion trap and also presented evidence for nucleophilic substitution reactions. In addition, they have examined the reactions of benzonitrile radical cations with nitrobenzenes and discovered an aromatic substitution pathway (Eq.…”

Section: Quadrupole Ion Trap Studies Of Fundamental Organic Reactionsmentioning

confidence: 99%

Quadrupole ion trap studies of fundamental organic reactions

Gronert

2004

Mass Spectrometry Reviews

128

133

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Over the past several decades, quadrupole ion traps have become important instruments in the study of gas phase ion/molecule reactions. As a part of this work, they have been employed in a number of studies focused on fundamental organic reaction mechanisms. In this review, the general features and limitations of quadrupole ion traps are discussed followed by a description of representative ion trap studies of organic reaction mechanisms such as substitution, elimination, and acyl transfer reactions.

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“…The maximum-entropy (MaxEnt) method has been proposed for charge state deconvolution of ESI spectra of complex mixtures using MassLynx MaxEnt 1 software (Waters) [26].…”

Section: Intact Mass Analysismentioning

confidence: 99%

Combination of SDS‐PAGE and intact mass analysis for rapid determination of heterogeneities in monoclonal antibody therapeutics

et al. 2017

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mAbs are currently mainstream in biopharmaceuticals, and their market has been growing due to their high target specificity. Characterization of heterogeneities in mAbs is performed to secure their quality and safety by physicochemical analyses. However, they require time-consuming task, which often strain the resources of drug development in pharmaceuticals. Rapid and direct method to determine the heterogeneities should be a powerful tool for pharmaceutical analysis. Considering the advantages of electrophoresis and MS, this study addresses the combination of SDS-PAGE and intact mass analysis, which provides direct, rapid, and orthogonal determination of heterogeneities in mAb therapeutics. mAb therapeutics that migrated in SDS-PAGE were recovered from gel by treatment with SDC-containing buffer. Usage of SDC-containing buffer as extraction solvent and ethanol-based staining solution enhanced the recovery of intact IgG from SDS-PAGE gels. Recovery of mAbs reached more than 86% with 0.2% SD. The heterogeneities, especially N-glycan variants in the recovered mAb therapeutics, were clearly determined by intact mass analysis. We believe that the study is important in pharmaceuticals‧ perspective since orthogonal combination of gel electrophoresis and intact mass analysis should be pivotal role for rapid and precise characterization of mAbs.

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Deamination of protonated amines to yield protonated imines

Cited by 20 publications

References 31 publications

Detection in human saliva of different statherin and P-B fragments and derivatives

Detection in human saliva of different statherin and P-B fragments and derivatives

Quadrupole ion trap studies of fundamental organic reactions

Combination of SDS‐PAGE and intact mass analysis for rapid determination of heterogeneities in monoclonal antibody therapeutics

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