Dearomatization of lignin derivatives by fungal protocatechuate 3,4‐dioxygenase immobilized on porosity glass

Wojtas-Wasilewska, M.; Luterek, Jolanta; Leonowicz, Andrzej; Dawidowicz, Andrzej L.

doi:10.1002/bit.260320413

Cited by 18 publications

(8 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immobilized 3,4-POD with high activity was obtained only after 2 h of incubation as shown in Supplementary Fig. S2 which was a greatly reduced time than the porous glass media (75 and 24 h) 18 19 and agarose matrices (75 and 18 h) 20 . In order to obtain higher 3,4-POD loading efficiency and activity yield, we investigated optimum weight ratio of 3,4-POD (0.02–2.0 mg) to F-MWCNTs (1.0 mg) as shown in Fig.…”

Section: Resultsmentioning

confidence: 98%

“…The MA has not shown toxicity effects on normal cells 16 , and is an industrially valuable compound for adipic acid production (2.3 million metric tons/year and expected market value was 6.3 billion pounds in 2012) 17 . A few studies have been attempted to immobilize 3,4-POD onto porous glass 18 19 , agarose 20 21 and alginate matrix 21 22 . However, the studies were done without proper scrutiny.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Highly Efficient and Stable Novel NanoBiohybrid Catalyst to Avert 3,4-Dihydroxybenzoic Acid Pollutant in Water

Das

Hamid

Annuar

2016

Sci Rep

View full text Add to dashboard Cite

The present study reported for the first time covalent immobilization of protocatechuate 3,4-dioxygenase (3,4-POD) onto functionalized multi-walled carbon nanotubes (F-MWCNT) for degrading the toxic 3,4-dihydroxybenzoic acid (3,4-DHBA) pollutant in water. The F-MWCNTs had a maximum 3,4-POD loading of 1060 μg/mg. Immobilized 3,4 POD had 44% of relative structural changes to its free configurations. Nevertheless, >90% of relative activity and about 50% of catalytic efficiency were retained to the free enzyme. Immobilized 3,4-POD demonstrated higher alkaline stability and thermostability than the free 3,4-POD. The free and immobilized 3,4-POD lost 82% and 66% of relative activities, respectively after 180 min of incubations at 90 °C. Excellent shelf-life was observed for the immobilized 3,4-POD with residual activity of 56% compared with 41% and 39% of the free 3,4-POD at 4 °C and 25 °C over 30 days storage. Immobilized 3,4-POD showed >60% of catalytic activity retention even after ten-cycle uses, defraying the expenses of free 3,4-POD productions for long term uses. Finally, the immobilized 3,4-POD removed 71% of 3,4-DHBA from water in <4 h, paving its future application for water purification with reduced costs and time.

show abstract

Section: Resultsmentioning

confidence: 98%

mentioning

confidence: 99%

Highly Efficient and Stable Novel NanoBiohybrid Catalyst to Avert 3,4-Dihydroxybenzoic Acid Pollutant in Water

Das

Hamid

Annuar

2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…White-rot fungi are also thought to degrade vanillate via the same route [274,322]. Indeed, intracellular protocatechuate 3,4-dioxygenase (EC 1.13.11.3) has been purified from P. ostreatus [323,324]. However, protocatechuate could not be detected as an intermediate in the metabolism of veratrate or vanillate by several basidiomycetes, including P. chrysosporium [279].…”

Section: Metabolism Of Aryl Alcohol Metabolites By Intracellular Enzymesmentioning

confidence: 99%

Aryl alcohols in the physiology of ligninolytic fungi

DEJONG¹,

FIELD²,

DEBONT³

1994

FEMS Microbiology Reviews

View full text Add to dashboard Cite

White-rot fungi have a versatile machinery of enzymes which work in harmony with secondary aryl alcohol metabolites to degrade the recalcitrant, aromatic biopolymer lignin. This review will focus on the important physiological roles of aryl (veratryl, anisyl and chlorinated anisyl) alcohols in the ligninolytic enzyme system. Their functions include stabilization of lignin peroxidase, charge-transfer reactions and as substrate for oxidases generating extracellular H20 2. The aryl alcohol/aldehyde couple is well protected against degradation by the fungi's extracellular ligninolytic enzymes and their concentrations in the extracellular fluid are highly regulated by intracellular enzymes.

show abstract

“…This could mean that some amount of 3,4-DHBA was transformed into β-CMA with the help of QsuB under these conditions due to dioxygenase activity of the C-terminal domain. The formation of β-CMA could be detected by UV spectroscopy [27]. The QsuB protein was incubated at pH 7.5 in the presence of 3,4-DHBA for up to 60 min, but no difference in the UV spectrum was observed (Fig 7).…”

Section: Resultsmentioning

confidence: 99%

“…The UV spectrum of the reaction mixture (pH 7.5) containing purified QsuB before and after 60 min incubation with 3,4-DHBA was the same and coincided with the 3,4-DHBA spectrum. The UV spectrum of β-CMA is shown in [27]. …”

Section: Resultsmentioning

confidence: 99%

Characterization of theCorynebacterium glutamicumdehydroshikimate dehydratase QsuB and its potential for microbial production of protocatechuic acid

Shmonova

Voloshina

Ovsienko

et al. 2020

Preprint

View full text Add to dashboard Cite

2 The dehydroshikimate dehydratase (DSD) from Corynebacterium glutamicum 3 encoded by the qsuB gene is related to the previously described QuiC1 protein 4 (39.9% identity) from Pseudomonas putida. QuiC1 and QsuB are both two-domain 5 bacterial DSDs. The N-terminal domain provides dehydratase activity, while the 6 C-terminal domain has sequence identity with 4-hydroxyphenylpyruvate 7 dioxygenase. Here, the QsuB protein and its DSD domain (N-QsuB) were 8 expressed in the T7 system, purified and characterized. QsuB was present mainly 9 in octameric form (60%), while N-QsuB had a predominantly monomeric structure 10 (80%) in solution. Both proteins possessed DSD activity with one of the following 11 cofactors (listed in order of decreasing activity): Co 2+ , Mg 2+ , Mn 2+ or Ca 2+ . The K m 12 and k cat values for QsuB were two and three times higher, respectively (K m ~ 1 13 mM, k cat ~ 61 s -1 ) than those for N-QsuB. Notably, 3,4-DHBA inhibited both 14 enzymes via an uncompetitive mechanism. QsuB and N-QsuB were tested for 3,4-15 DHBA production from glucose in E. coli. MG1655aroE P lac qsuB produced at 16 least two times more 3,4-DHBA than MG1655aroE P lac n-qsuB in the presence 17 of isopropyl β-D-1-thiogalactopyranoside.18

show abstract

Dearomatization of lignin derivatives by fungal protocatechuate 3,4‐dioxygenase immobilized on porosity glass

Cited by 18 publications

References 17 publications

Highly Efficient and Stable Novel NanoBiohybrid Catalyst to Avert 3,4-Dihydroxybenzoic Acid Pollutant in Water

Highly Efficient and Stable Novel NanoBiohybrid Catalyst to Avert 3,4-Dihydroxybenzoic Acid Pollutant in Water

Aryl alcohols in the physiology of ligninolytic fungi

Characterization of theCorynebacterium glutamicumdehydroshikimate dehydratase QsuB and its potential for microbial production of protocatechuic acid

Contact Info

Product

Resources

About