Deblurring of 3-dimensional patterns of evoked rat cerebellar cortical activity: a study using voltage-sensitive dyes and optical sectioning

Yae, H.; Elias, Stephen A.; Ebner, Timothy J.

doi:10.1016/0165-0270(92)90099-y

Cited by 17 publications

(10 citation statements)

References 25 publications

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“…The availability of three-dimensional imaging procedures for the light microscope (Agard and Sedat, 1983;Agard, 1984;Macias Garza et al, 1989;Hiraoka et al, 1990) has already suggested that optical serial sectioning of voltage-sensitive dye signals through a tissue can be used to determine the three-dimensional spatial structure of the electrical activity (Grinvald et al, 1984(Grinvald et al, , 1988Cohen and Lesher, 1986;Yae et al, 1992). Threedimensional microscopic data are collected as a series of twodimensional signal images recorded at different focal planes, so that each of these signal images contains not only in-focus information from the positions around the focal plane, but also out-of-focus contributions from the remainder of the preparation: the results of optical sectioning measurements result in a distorted view of the spatial distribution of the signal.…”

Section: Discussionmentioning

confidence: 99%

Responses to glossopharyngeal stimulus in the early embryonic chick brainstem: spatiotemporal patterns in three dimensions from repeated multiple-site optical recording of electrical activity

et al. 1995

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In an effort to assess the spatial patterning of glossopharyngeal responses in the early embryonic chick brainstem, we used a multiple- site optical recording system with a 12 x 12 element photodiode array and a voltage-sensitive merocyanine-rhodanine dye (NK2761) to monitor neural transmembrane voltage activities. Seven and 8 d old embryonic chick brainstems were sliced into 1400–1600 microns thick sections with the glossopharyngeal and vagal nerves attached, and then stained with the dye. Neural voltage-related optical signals were evoked by a positive brief (depolarizing) square current pulse applied to the glossopharyngeal nerve with a microsuction electrode, and then recorded simultaneously from many loci in the objective two-dimensional image plane of a compound microscope. In addition to the multiple-site optical recording technique, we tried to introduce an optical sectioning method by changing the focal plane of the microscope to obtain three-dimensional information. Thus, we have been able to assess semiquantitatively the three-dimensional profiles of two glossopharyngeal response areas corresponding to the nucleus of the glossopharyngeal nerve (nucleus nervi glossopharyngei) and the nucleus of the tractus solitarius. Furthermore, glutaminergic excitatory postsynaptic potentials were determined within the response area corresponding to the nucleus of the tractus solitarius. In addition, we also compared the glossopharyngeal and vagal response areas and found that the cores of the related nuclei are separated in three dimensions.

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Section: Discussionmentioning

confidence: 99%

Responses to glossopharyngeal stimulus in the early embryonic chick brainstem: spatiotemporal patterns in three dimensions from repeated multiple-site optical recording of electrical activity

et al. 1995

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“…In order to estimate the optical distortion occurring in our preparations, empirical point spread functions (PSF) were generated by collecting light at different depths (in 0.8 μm steps) from fluorescent beads (0.04-μm diameter) injected into the granular and molecular layer in sagittal and coronal cerebellar slices (Yae et al, 1992) and processing signals off-line (Image-J). In the granular layer, which is almost isotropic, PSF analysis showed that light signals vanished in ∼10 μm both vertically and horizontally.…”

Section: Methodsmentioning

confidence: 99%

High-pass filtering and dynamic gain regulation enhance vertical bursts transmission along the mossy fiber pathway of cerebellum

Mapelli¹

2010

Front. Cell. Neurosci.

111

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Signal elaboration in the cerebellum mossy fiber input pathway presents controversial aspects, especially concerning gain regulation and the spot-like (rather than beam-like) appearance of granular to molecular layer transmission. By using voltage-sensitive dye imaging in rat cerebellar slices (Mapelli et al., 2010), we found that mossy fiber bursts optimally excited the granular layer above ∼50 Hz and the overlaying molecular layer above ∼100 Hz, thus generating a cascade of high-pass filters. NMDA receptors enhanced transmission in the granular, while GABA-A receptors depressed transmission in both the granular and molecular layer. Burst transmission gain was controlled through a dynamic frequency-dependent involvement of these receptors. Moreover, while high-frequency transmission was enhanced along vertical lines connecting the granular to molecular layer, no high-frequency enhancement was observed along the parallel fiber axis in the molecular layer. This was probably due to the stronger effect of Purkinje cell GABA-A receptor-mediated inhibition occurring along the parallel fibers than along the granule cell axon ascending branch. The consequent amplification of burst responses along vertical transmission lines could explain the spot-like activation of Purkinje cells observed following punctuate stimulation in vivo.

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“…In searching for less phototoxic 180 A.L. Obaid et al / Journal of Neuroscience Methods 134 (2004) 179-190 and more soluble dyes with large voltage-sensitive fluorescence signals, we first assayed the dienylstyryl-pyridinium dye RH795, originally synthesized by Grinvald et al (1994) and now available from molecular probes (R649), which is highly water soluble and has already found numerous applications in voltage-sensitive dye recordings of electrical activity (Albowitz and Kuhnt, 1993;Contreras and Llinas, 2001;Maeda et al, 2001;Yae et al, 1992). Because we found that RH795 is readily internalized by the neurons of the submucous plexus, we attempted to generate chimeric structures that retained the best features of both of these dyes.…”

Section: Introductionmentioning

confidence: 99%

Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis

Obaid

Loew

Wuskell

et al. 2004

Journal of Neuroscience Methods

121

108

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Deblurring of 3-dimensional patterns of evoked rat cerebellar cortical activity: a study using voltage-sensitive dyes and optical sectioning

Cited by 17 publications

References 25 publications

Responses to glossopharyngeal stimulus in the early embryonic chick brainstem: spatiotemporal patterns in three dimensions from repeated multiple-site optical recording of electrical activity

Responses to glossopharyngeal stimulus in the early embryonic chick brainstem: spatiotemporal patterns in three dimensions from repeated multiple-site optical recording of electrical activity

High-pass filtering and dynamic gain regulation enhance vertical bursts transmission along the mossy fiber pathway of cerebellum

Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis

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