1983
DOI: 10.1271/bbb1961.47.771
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Debranching enzymes of potato tubers (Solanum tuberosum L.). I. Purification and some properties of potato isoamylase.

Abstract: Potato tubers contain 3 debranching enzymes separable by polyacrylamide gel electrophoresis. One of them, isoamylase, has been purified to apparent homogeneity on disc gel electrophoresis by isoelectric precipitation, fractionation with ammoniumsulfate, gel filtration on Sepharose 6B and finally affinity chromatography on Sepharose 4B-soluble starch, successively. The purified enzyme has a specific activity of 8.0U/mg of protein. It hydrolyzes the a-l,6-glucosidic bonds in glycogen and phytoglycogen not only a… Show more

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Cited by 35 publications
(27 citation statements)
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“…As reported previously for the enzyme from potato (Ishizaki et al, 1983), activity was effectively precipitated at pH 5.0, whereas the activity of other starch-hydrolyzing enzymes was not. Activity of the putative isoamylase was monitored throughout the purification by analysis on native gels containing amylopectin.…”
Section: Purification Of Isoamylase From Developing Embryossupporting
confidence: 80%
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“…As reported previously for the enzyme from potato (Ishizaki et al, 1983), activity was effectively precipitated at pH 5.0, whereas the activity of other starch-hydrolyzing enzymes was not. Activity of the putative isoamylase was monitored throughout the purification by analysis on native gels containing amylopectin.…”
Section: Purification Of Isoamylase From Developing Embryossupporting
confidence: 80%
“…A single form of the enzyme purified from potato tuber hydrolyzed glycogen and amylopectin at an almost equal rate (Ishizaki et al, 1983). One of two forms of the enzyme separated by anion-exchange chromatography from developing maize kernels hydrolyzed amylopectin about four times faster than phytoglycogen, and the other form was not characterized (Doehlert and Knutson, 1991).…”
Section: The Isoamylase Of Developing and Germinating Embryosmentioning
confidence: 99%
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“…Higher plant isoamylase-type DBEs are multimeric, with molecular masses of approximately 340 to 500 kD for both the potato tuber and rice endosperm enzymes (Ishizaki et al, 1983;Fujita et al, 1999). This compares to the 83-kD monomer size generally conserved in plants, as shown by immunoblot analysis of crude cell lysates.…”
Section: Dbes and Their Involvement In Amylopectin Biosynthesismentioning
confidence: 88%
“…Are there other isoforms of isoamylase in sweetpotato? Two types of isoamylases (with a molecular mass of 68 and 140 kDa) were purified from developing maize kernels (Doehlert and Knutson 1991) and one type (with a monomeric molecular mass of 80-95 kDa) from potato tubers (Ishizaki et al 1983). Southern hybridization suggested the presence of at least two copies of IbIsa1 in Kokei 14.…”
Section: Discussionmentioning
confidence: 99%