Decaploidy in <i>Fragaria iturupensis</i> (Rosaceae)

Hummer, Kim E.; Nathewet, Preeda; Yanagi, Tetsuo

doi:10.3732/ajb.0800285

Cited by 55 publications

(28 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10,11 The geographic origins of the wild species are distributed throughout Eurasia, North and South America, and Japan. Fragaria x ananassa originated in the 1700s from a natural hybridization between two octoploids, F. virginiana and F. chiloensis .…”

Section: Introductionmentioning

confidence: 99%

Dissection of the Octoploid Strawberry Genome by Deep Sequencing of the Genomes of Fragaria Species

et al. 2013

View full text Add to dashboard Cite

Cultivated strawberry (Fragaria x ananassa) is octoploid and shows allogamous behaviour. The present study aims at dissecting this octoploid genome through comparison with its wild relatives, F. iinumae, F. nipponica, F. nubicola, and F. orientalis by de novo whole-genome sequencing on an Illumina and Roche 454 platforms. The total length of the assembled Illumina genome sequences obtained was 698 Mb for F. x ananassa, and ∼200 Mb each for the four wild species. Subsequently, a virtual reference genome termed FANhybrid_r1.2 was constructed by integrating the sequences of the four homoeologous subgenomes of F. x ananassa, from which heterozygous regions in the Roche 454 and Illumina genome sequences were eliminated. The total length of FANhybrid_r1.2 thus created was 173.2 Mb with the N50 length of 5137 bp. The Illumina-assembled genome sequences of F. x ananassa and the four wild species were then mapped onto the reference genome, along with the previously published F. vesca genome sequence to establish the subgenomic structure of F. x ananassa. The strategy adopted in this study has turned out to be successful in dissecting the genome of octoploid F. x ananassa and appears promising when applied to the analysis of other polyploid plant species.

show abstract

Section: Introductionmentioning

confidence: 99%

Dissection of the Octoploid Strawberry Genome by Deep Sequencing of the Genomes of Fragaria Species

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Fragaria , which encompasses all soft-fruited strawberry species, is comprised of a limited number of species ranging from diploids (2 n = 2 x = 14) to decaploid (2 n = 8 x = 56) (Staudt 1989, 2009; Hummer et al 2009). It is belonging to the Rosaceae family, which comprises many economically important species such as the apple, peach, and plum and to the Rosodeae supertribe (Potter et al 2007) which includes various edible and cultivated berries (e.g.…”

Section: Introductionmentioning

confidence: 99%

Genetic dissection of fruit quality traits in the octoploid cultivated strawberry highlights the role of homoeo-QTL in their control

et al. 2012

View full text Add to dashboard Cite

Fruit quality traits are major breeding targets in the Rosaceae. Several of the major Rosaceae species are current or ancient polyploids. To dissect the inheritance of fruit quality traits in polyploid fleshy fruit species, we used a cultivated strawberry segregating population comprising a 213 full-sibling F1 progeny from a cross between the variety ‘Capitola’ and the genotype ‘CF1116’. We previously developed the most comprehensive strawberry linkage map, which displays seven homoeology groups (HG), including each four homoeology linkage groups (Genetics 179:2045–2060, 2008). The map was used to identify quantitative trait loci (QTL) for 19 fruit traits related to fruit development, texture, colour, anthocyanin, sugar and organic acid contents. Analyses were carried out over two or three successive years on field-grown plants. QTL were detected for all the analysed traits. Because strawberry is an octopolyploid species, QTL controlling a given trait and located at orthologous positions on different homoeologous linkage groups within one HG are considered as homoeo-QTL. We found that, for various traits, about one-fourth of QTL were putative homoeo-QTL and were localised on two linkage groups. Several homoeo-QTL could be detected the same year, suggesting that several copies of the gene underlying the QTL are functional. The detection of some other homoeo-QTL was year-dependent. Therefore, changes in allelic expression could take place in response to environmental changes. We believe that, in strawberry as in other polyploid fruit species, the mechanisms unravelled in the present study may play a crucial role in the variations of fruit quality.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-011-1769-3) contains supplementary material, which is available to authorized users.

show abstract

“…Historically, a basic chromosome number of seven ( x = 7) and the existence of multiple levels of ploidy, ranging from diploid to octoploid, in this genus had been documented by 1926 [3,4]. Only recently, a naturally occurring decaploid cytotype (2 n = 10 x = 70) was revealed through chromosome counting and flow cytometry [5] in the geographically isolated species F. iturupensis , which had initially been described as an octoploid (2 n = 8 x = 56) [6]. In addition, an accession (CFRA 110) of F .…”

Section: Introductionmentioning

confidence: 99%

“…Currently, twelve diploid (2 n = 2 x = 14), five tetraploid (2 n = 4 x = 28), a single hexaploid (2 n = 6 x = 42) F. moschata , three octoploid (2 n = 8 x = 56) species, including the main cultivated species F . × ananassa and its immediate octoploid ancestors: F. chiloensis and F. virginiana [8], and a decaploid (2 n = 10 x = 70) F. iturupensis are recognized [1,5,9,10]. In addition, variable- and odd-ploidy have been recognized in two hybrid taxa: pentaploid/hexaploid/enneaploid (2 n = 5 x /6 x /9 x = 35/42/63) F .…”

Section: Introductionmentioning

confidence: 99%

Conservation and loss of ribosomal RNA gene sites in diploid and polyploid Fragaria (Rosaceae)

Liu

Davis

2011

BMC Plant Biol

View full text Add to dashboard Cite

BackgroundThe genus Fragaria comprises species at ploidy levels ranging from diploid (2n = 2x = 14) to decaploid (2n = 10x = 70). Fluorescence in situ hybridization with 5S and 25S rDNA probes was performed to gather cytogenetic information that illuminates genomic divergence among different taxa at multiple ploidy levels, as well as to explore the evolution of ribosomal RNA genes during polyploidization in Fragaria.ResultsRoot tip cells of diploid taxa were typified by two 5S and six 25S rDNA hybridization signals of varying intensities, providing a baseline for comparisons within the genus. In three exceptional diploid genotypes, F. nilgerrensis (CFRA 1358 and CFRA 1825) and F. vesca 'Yellow Wonder', two 5S but only four 25S rDNA sites were found but with differing site losses. The numbers of 5S and 25S rDNA signals, respectively were three and nine in a triploid F. ×bifera accession, and were four and twelve in three tetraploids, thus occurring in proportional 1.5× and 2× multiples of the typical diploid pattern. In hexaploid F. moschata, a proportional multiple of six 5S rDNA sites was observed, but the number of 25S rDNA sites was one or two less than the proportionate prediction of eighteen. This apparent tendency toward rDNA site loss at higher ploidy was markedly expanded in octoploids, which displayed only two 5S and ten 25S rDNA sites. In the two decaploids examined, the numbers of 5S and 25S rDNA signals, respectively, were four and fifteen in F. virginiana subsp. platypetala, and six and twelve in F. iturupensis.ConclusionsAmong diploid Fragaria species, a general consistency of rDNA site numbers implies conserved genomic organization, but highly variable 25S signal sizes and intensities and two instances of site loss suggest concurrent high dynamics of rDNA copy numbers among both homologs and non-homologs. General conservation of rDNA site numbers in lower ploidy, but marked site number reductions at higher ploidy levels, suggest complex evolution of rDNA sites during polyploidization and/or independent evolutionary pathways for 6x versus higher ploidy strawberries. Site number comparisons suggest common genomic composition among natural octoploids, and independent origins of the two divergent decaploid accessions.

show abstract

Decaploidy in Fragaria iturupensis (Rosaceae)

Cited by 55 publications

References 18 publications

Dissection of the Octoploid Strawberry Genome by Deep Sequencing of the Genomes of Fragaria Species

Dissection of the Octoploid Strawberry Genome by Deep Sequencing of the Genomes of Fragaria Species

Genetic dissection of fruit quality traits in the octoploid cultivated strawberry highlights the role of homoeo-QTL in their control

Conservation and loss of ribosomal RNA gene sites in diploid and polyploid Fragaria (Rosaceae)

Contact Info

Product

Resources

About