Decarboxylase activity involved in methyl ketone production by Staphylococcus carnosus 833, a strain used in sausage fermentation

Aims: The growth and survival of Staphylococcus xylosus and Staphylococcus carnosus were monitored during sausage manufacture in two processing plants. Methods and Results: The gram‐positive, catalase‐positive cocci isolated from the processing plants F10 and F11 were identified by Staphylococcus‐specific PCR and species‐specific oligonucleotide array. In the inoculated products with starter cultures, 90% of staphylococcal strains isolated in F10 were identified as S. xylosus and 10% as S. carnosus. In F11, 77% were identified as S. xylosus and 20% as S. carnosus. Staphylococcus xylosus dominated the staphylococcal microbiota while S. carnosus survived during the process. The pulse‐field gel electrophoresis analysis revealed that all S. xylosus and S. carnosus strains isolated corresponded to the starter strains inoculated. The two starter strains of S. xylosus co‐dominated in the isolates from sausages of F11, whereas the strain with pattern A1 was dominant in the isolates from sausages of F10. In the environments, no S. carnosus and S. xylosus were found, whereas Staphylococcus equorum and Staphylococcus saprophyticus were the main species isolated. Conclusions: This work highlighted the domination of S. xylosus starter strains, which showed a strong capacity to grow during sausage process, while S. carnosus survived during the process. Significance and Impact of the Study: Successful implantation of starter cultures is obviously a prerequisite for their contribution to sensorial qualities. Thus, the monitoring of the growth and the survival of S. xylosus and S. carnosus are required to guarantee a well‐adapted starter culture. This study revealed that the two Staphylococcus species are suitable for manufacturing sausages in processing plants with very different capacities of production.

show abstract

“…2001). Staphylococcus carnosus was shown to be able to produce methyl ketones from an incomplete β ‐oxidation of fatty acids (Fadda et al. 2002).…”

Section: Introductionmentioning

confidence: 99%

Monitoring of staphylococcal starters in two French processing plants manufacturing dry fermented sausages

2007

View full text Add to dashboard Cite

show abstract

“…Concerning S. carnosus , Engelvin et al [3] have reported the existence of a β‐oxidative pathway and the liberation of intermediates from a β‐oxidation cycle. Recently, we have reported a β‐decarboxylase activity in resting cells of S. carnosus using a β‐ketoacid as substrate [4,5]. These results show that S. carnosus , as do fungi, may produce ketones via this pathway.…”

Section: Introductionmentioning

confidence: 68%

“…Since a β‐decarboxylase activity involved in methyl ketone formation was detected for the first time in resting cells of S. carnosus [5], the objective of this work was to characterize the β‐decarboxylase activities present in cell‐free extract (CFE) of S. carnosus 833 strain. The optimal conditions for enzyme activities such as temperature, pH, influence of potential cofactors and/or inhibitors, effect of technological conditions and different substrate affinities were studied.…”

Section: Introductionmentioning

confidence: 99%

Preliminary characterization of Î²-decarboxylase activities inStaphylococcus carnosus833, a strain used in sausage fermentation

Fadda¹,

Leroy-Sétrin²,

Talon³

2003

FEMS Microbiology Letters

View full text Add to dashboard Cite

In sausage, Staphylococcus carnosus increases the levels of methyl ketones which could arise from incomplete L-oxidation of fatty acids followed by a decarboxylation. The objective of this work was to characterize the L-decarboxylase activities in cell-free extract. By using different substrates, at least two kinds of L-decarboxylase activities were shown: an acetoacetate-like and an oxaloacetate-like. The first one leads to the production of ketones from ethylbutyryl acetate or acetoacetic acid. The activity was optimal at pH 6.0, stimulated by pyridoxal phosphate but ethylenediamine tetraacetic acid (EDTA), NaCl, iodoacetate and curing additives were inhibitory. The second decarboxylase activity leads to the production of pyruvic acid from oxaloacetic acid. This activity was optimal at pH 5.0 and stimulated by divalent ions and biotin.

show abstract

“…The traditional use of these food grade staphylococci has been as starter cultures in the ripening process of dry sausages (Liepe, 1982), and strains of S. carnosus have been isolated from fermented meat products throughout the world (Hammes et al, 1995;Fadda et al, 2002). Both strains are characterized as nonsporulating, nonmotile cocci, and grow predominantly in pairs or singly (Schleifer and Kloos, 1975;Schleifer and Fischer, 1982).…”

Section: Staphylococcal Surface Display and Its Applicationsmentioning

confidence: 99%

Engineering of staphylococcal surfaces for biotechnological applications

Wernérus

Lehtiö

Samuelson

et al. 2002

Journal of Biotechnology

View full text Add to dashboard Cite

The engineering of bacterial surfaces has in recent years attracted a lot of attention with applications in many different areas of bioscience. Here we describe the use of two different surface display systems for the gram-positive bacteria Staphylococcus carnosus and Staphylococcus xylosus in various biotechnological applications.Environmental microbiology currently attracts a lot of attention since genetically engineered plants and bacteria might be used as bioadsorbents for sequestration of toxic metals. Bacterial surface display of metal-binding peptides might enable recycling of the biomass by desorption of accumulated heavymetals. In an attempt to recruit staphylococcal display systems for bioremediation purposes, polyhistidyl peptides were successfullly displayed on the surface of recombinant S. carnosus and S. xylosus cells. Whole-cell Ni 2+ -binding assays demonstrated that the recombinant cells had gained metal-binding capacity compared to wild-type cells.Tailor-made, metal-binding staphylococci was created using a previously constructed phage-display combinatorial protein library based on a fungal cellulose-binding domain (CBD) derived from the cellobiohydrolase Cel7A of Trichoderma reseii. Novel metal-binding CBDs were generated through a phage mediated selection procedure. Selected CBD variants, now devoid of cellulose binding, were randomly selected and sequence analysis of selected variants revealed a marked preference for histidine residues at the randomized positions. Surface display of these novel CBD variants resulted in recombinant staphylococci with increased metal-binding capacity compared to control strains, indicating that this could become a general strategy to engineer bacteria for improved binding to specific metal ions.Directed immobilization of cells with surface displayed heterologous proteins have widespread use in modern biotechnology. Among other things they could provide a convenient way of generating biofilters, biocatalysts or whole-cell diagnostic devices. It was therefore investigated whether directed immobilization of recombinant staphylococci on cotton fibers could be achieved by functional display of a fungal cellulose-binding domain (CBD). Recombinant S. carnosus cells with surface anchored CBDs from Trichoderma reseii Cel6A were found to efficiently bind to cotton fibers creating almost a monolayer on the fibrous support. The co-expression of this CBD together with previously described metal-binding proteins on the surface of our staphylococci would create means for developing effective bioadsorbents for remediation purposes.The original plasmid vector, designed for heterologous surface display on recombinant S. carnosus cells has exhibited problems related to structural instability, possibly due to the presence of a phage f1 origin of replication in the vector sequence. This would be a problem if using the vector system for library display applications. Therefore, novel surface display vectors, lacking the phage ori were constructed and evaluated by enzymatic a...

show abstract

Decarboxylase activity involved in methyl ketone production by Staphylococcus carnosus 833, a strain used in sausage fermentation

Cited by 12 publications

References 19 publications

Monitoring of staphylococcal starters in two French processing plants manufacturing dry fermented sausages

Monitoring of staphylococcal starters in two French processing plants manufacturing dry fermented sausages

Preliminary characterization of Î²-decarboxylase activities inStaphylococcus carnosus833, a strain used in sausage fermentation

Engineering of staphylococcal surfaces for biotechnological applications

Contact Info

Product

Resources

About