Decay of Root Debris after Harvesting American Ginseng (Panax quinquefolius) and Changes in Soil Chemistry and Microbiology

Suárez, Iván Darío Samur; Valliani, Moez; Hsiang, Tom; Goodwin, Paul H.

doi:10.3390/soilsystems7040108

Cited by 3 publications

(1 citation statement)

References 42 publications

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“…However, when compared with microbial communities from 12 collection points, Pseudoflavonifractor associated with diseased rhizomes was not detected, indicating that samples from the 12 collection points were all healthy (Cao et al, 2023). Additionally, the microbial community of American ginseng with different growth ages had some impact but did not alter the core microbial community (Suárez et al, 2023). Future research should focus on further validating the selected dominant microbial communities associated with PCH.…”

Section: Discussionmentioning

confidence: 95%

Quality analysis and function prediction of soil microbial communities of Polygonatum cyrtonema in two indigenous-origins

Yang,

Wulu

et al. 2024

Front. Microbiol.

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Polygonatum cyrtonema Hua (PCH), as an important economic crop, is used as raw industrial materials and traditional Chinese medicine. There are significant variations in the quality of PCH from different geographical origins. It can be due to the change of the endophytic fungi and soil microbial communities of PCH. Therefore, the aim of this study is to investigate the composition and functional prediction of the main microbial communities in the rhizomes and soil of PCH and explore their impact on medicinal quality. High-throughput sequencing techniques targeting ITS and 16S rDNA were employed to compare the structure and biodiversity differences of endophytic fungi in the rhizomes and soil microbial communities of PCH from 12 different locations in Sichuan and Guangxi province. Heatmap analysis was used for comprehensive statistics and visualization of the richness of rhizome and soil microbial communities from all locations. Venn analysis was conducted to determine the total number of shared fungi between rhizomes and soil, and GraphPad Prism analysis was employed to predict and compare the microbial communities related to phenotypes at the genus level in Sichuan and Guangxi. Tax4Fun and Fungild were used for metabolic function prediction of microbial communities in the rhizomes and soil of PCH. The results revealed the identification of 19,387 bacterial amplicon sequence variants (ASVs) in the rhizomes and 37,990 bacterial ASVs in the soil, with 6,889 shared bacterial ASVs. In addition, 2,948 fungal ASVs were identified in the rhizomes and 8,868 in the soil, with 1,893 shared fungal ASVs. Microbial sequencing results indicated that the fungal communities between soil and rhizomes were mainly composed of Ascomycota and Basidiomycota, while bacterial communities included Proteobacteria, Acidobacteria, Bacteroidota, Gammatimonadota, and Firmicutes. Dominant bacterial groups such as Nitrospira, Acidibacter, and fungal groups including Mortierella, Ceratobasidium, and Fusarium were identified as potential contributors to the observed traits. In the top 15 microbial genera, both Sichuan and Guangxi contain 15 bacterial genera, but there are differences in their abundance. Guangxi has three unique fungal genera, including the genera Scleroderma, Russula, and Gliocladiopsis. On the other hand, Sichuan has the unique fungal genus Chamaeota. The correlation analysis between the microbiota and the chemical content from 12 different collecting spots was performed by GraphPad Prism. Burkholderia-Caballeronia-Paraburkholderia, Acidibacter, and Amycolatopsis show an inverse proportionality to total polysaccharides and saponins, while Enterobacter shows a direct proportionality to total polysaccharides and inverse proportionality to saponins. The metabolism pathways show a significant positive correlation with PCH polysaccharides and saponins. This study provide new insights into the mechanisms underlying the quality differences between the two major indigenous areas.

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Section: Discussionmentioning

confidence: 95%

Quality analysis and function prediction of soil microbial communities of Polygonatum cyrtonema in two indigenous-origins

Yang,

Wulu

et al. 2024

Front. Microbiol.

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Cell-Free Extracts of the Ginseng Soil Bacterium Pseudomonas plecoglossicida Promote Suppression of Resistance of American Ginseng (Panax quinquefolius) to Root Rot Caused by Ilyonectria mors-panacis

Goodwin,

Hsiang

2024

Biology

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A prior report showed that soil previously planted with American ginseng (Panax quinquefolius) contained compound(s) which could reduce ginseng resistance to root infection by Ilyonectria mors-panacis, and this was not found in extracts from ginseng roots or soils not previously planted with ginseng. However, the origin of this ginseng-related factor in ginseng soils is unknown. An isolate of Pseudomonas plecoglossicida obtained from soil where P. quinquefolius had been harvested grew more in culture media when ginseng root extract was included, indicating the use of compounds in the extract as nutrients. Treatment with cell-free extracts from media containing ginseng root extracts where P. plecoglossicida had been cultured resulted in root lesions caused by I. mors-panacis being significantly larger than roots treated with fresh media containing root extract or with cell-free media inoculated with the same bacterial isolate without root extract. Levels of ginsenosides in the media decreased over time with incubation. Genome sequencing revealed that the bacterium had genes homologous to those reported for ginsenoside metabolism, which can release sugars for microbial growth. Thus, a ginseng soil bacterium, P. plecoglossicida, can create compound(s) suppressive to root rot resistance, similar to that found in soils previously planted with ginseng, indicating that the activity suppressing root rot resistance in soil previously planted with ginseng may be of microbial origin, utilizing compounds from ginseng roots.

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Appearance and Persistence of Activity in Soil Extracts Increasing Root Rot of American Ginseng (Panax quinquefolius) by Ilyonectria mors-panacis

Goodwin,

Zaro

2024

Soil Systems

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A previous report showed that methanol extracts from soil collected one year after harvesting American ginseng (Panax quinquefolius) contained activity that increased root rot caused by Ilyonectria mors-panacis. This effect was associated with suppression of the defense responses of P. quinquefolius. The activity was undetectable in soil not previously planted with ginseng, and it was hypothesized that it may be a factor in the development of ginseng replant disease (GRD). GRD can persist for 30 or more years and is associated with root rot from I. mors-panacis. A survey of activity that increases root rot was made of the soil at different times before and after commercial ginseng root harvesting. No activity that increased root rot from I. mors-panacis was detected in the soil of a first American ginseng crop over the three years from planting until prior to harvesting. After harvesting the first crop, no activity was detected during the fall or early spring, but I. mors-panacis’s ability to increase root rot was detected in the soil during late spring, when ginseng crop debris from the first crop had almost completely decayed and the soil had warmed. Activity increasing root rot from I. mors-panacis was also detected in the soil from 1 to 30 years after ginseng harvesting. These results indicate that activity in soil that increases root rot from I. mors-panacis is not detectable until after the crop has been first harvested and then can persist for many years, which is consistent with the long persistence of GRD.

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Decay of Root Debris after Harvesting American Ginseng (Panax quinquefolius) and Changes in Soil Chemistry and Microbiology

Cited by 3 publications

References 42 publications

Quality analysis and function prediction of soil microbial communities of Polygonatum cyrtonema in two indigenous-origins

Quality analysis and function prediction of soil microbial communities of Polygonatum cyrtonema in two indigenous-origins

Cell-Free Extracts of the Ginseng Soil Bacterium Pseudomonas plecoglossicida Promote Suppression of Resistance of American Ginseng (Panax quinquefolius) to Root Rot Caused by Ilyonectria mors-panacis

Appearance and Persistence of Activity in Soil Extracts Increasing Root Rot of American Ginseng (Panax quinquefolius) by Ilyonectria mors-panacis

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