Decellularization of donor pancreatic fragment to obtain a tissue-specific matrix scaffold

Пономарева, А. С.; Кирсанова, Л. А.; Баранова, Н. В.; Surguchenko, V. A.; Бубенцова, Г. Н.; Басок, Ю. Б.; Милосердов, И. А.; Sevastianov, V. I.

doi:10.15825/1995-1191-2020-1-123-133

Cited by 5 publications

(3 citation statements)

References 10 publications

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“…At the same time, the longer the period of chemical and enzymatic effects, the higher the probability of a damage to the components of the extracellular matrix. An alternative approach is to obtain a decellularized scaffold from fragments of the pancreas [14,29]. Such a strategy may be promising for tissue engineering technologies due to the minimization of a possible damage to proteins and the overall structure of the matrix, as well as the simplicity of its implementation.…”

Section: Discussionmentioning

confidence: 99%

“…The tissue-specific scaffolds most preferred for islet cells can be obtained by decellularization of the whole pancreas, as well as of the fragments of pancreatic tissue [13,14]. When developing protocols for pancreatic decellularization, it is important to take into account the maintenance of the structural, biochemical and biomechanical properties of the native ECM, the preservation of the architectonics with the maximum complete removal of cellular material (including DNA), and cellular surface antigens to minimize the immune response during implantation [15,16].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Analysis of the Influence of Extracellular Matrix Biomimetics on the Viability and Insulin-Producing Function of Isolated Pancreatic Islets

2021

JGEBR

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The creation of a pancreas tissue-engineered construct based on isolated pancreatic islets is hindered by problems associated with maintaining their viability and insulin-producing function. Both biopolymer and tissue-specific scaffolds can contribute to the maintenance of the structure and function of pancreatic islets in vitro and in vivo. A comparative morphofunctional analysis in vitro of isolated pancreatic islets cultured with a biopolymer collagen-containing scaffold and a tissue-specific scaffold obtained as a result of pancreatic decellularization was performed. The results showed that the use of the scaffolds contributes not only to the maintenance of the cultured islets viability, but also to the prolongation of their insulin-producing functions, compared to the islets monoculture in vitro. A significant increase was found in basal and stimulated (under glucose loading) insulin secreted by the islets cultured with the scaffolds. At the same time, the advantage of using a tissue-specific scaffold in comparison with a biopolymer collagen-containing scaffold was shown. We think that these studies will become a platform for creating a human pancreas tissue-engineered design for the treatment of type 1 diabetes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of the Influence of Extracellular Matrix Biomimetics on the Viability and Insulin-Producing Function of Isolated Pancreatic Islets

2021

JGEBR

View full text Add to dashboard Cite

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“…In recent years, the development of cell and tissue-engineered constructs based on tissue-speci c scaffolds made from decellularized tissues, preserving the biochemical, spatial and vascular relationships of the native ECM, with the most complete removal of DNA, cellular material, and cellular surface antigens, has begun [26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Culture Systems of Isolated Pancreatic Islets with Extracellular Matrix Biomimetics as Tissue-Engineered Constructs of the Pancreas

Sevastianov

Баранова

Кирсанова

et al. 2021

Preprint

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The creation of a tissue-engineered structure of the pancreas based on isolated pancreatic islets is hindered by problems associated with maintaining their viability and insulin-producing function. Both biopolymer and tissue-specific scaffolds can contribute to the preservation of the structure and function of pancreatic islets in vitro and in vivo. Comparative morphofunctional analysis in vitro of two different types of tissue-engineered structures of the pancreas, which represent culture systems of isolated islets with biomimetics of an extracellular matrix - a biopolymer collagen-containing scaffold and a tissue-specific scaffold obtained as a result of pancreatic decellularization, - was performed. The results showed that the use of scaffolds in the creation of a tissue-engineered design of the pancreas contributes not only to the preservation of the viability of the islets, but also to the prolongation of their insulin-producing functions, compared to the monoculture of the islets in vitro. A significant increase was found in the basal and stimulated (under glucose load) insulin concentrations in the tissue of engineered structures studied, at the same time the advantage of using a tissue-specific scaffold compared to a biopolymer collagen-containing scaffold was shown. We think that these studies will become a platform for creating a tissue-engineered design of the human pancreas for treatment of type 1 diabetes mellitus.

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Determining the optimal pancreatic decellularization protocol, taking into account tissue morphological features

Пономарева

Баранова

Кирсанова

et al. 2022

RJTAO

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Introduction. Developing a tissue-engineered pancreatic construct (TEPC) involves a search for matrices/scaffolds capable of mimicking the structure and composition of the natural extracellular matrix (ECM), which is an important component of the tissue microenvironment. A cell-free, tissue-specific matrix obtained from pancreas decellularization seems to be the most suitable for creation of a TEPC. The choice of pancreatic tissue decellularization protocol should take into account the morphological characteristics of the original pancreas. Preservation of the architectonics and composition of the native tissue in the decellularized pancreas matrix (DPM), and the presence of native ECM components allow for creation of conditions for prolonged vital activity of functionally active islet (insulin-producing) cells when creating TEPC.Objective: to determine the optimal parameters for decellularization of deceased donor pancreas with fibrosis, lipomatosis, and without pronounced signs of fibrosis and lipomatosis.Materials and methods. We used the caudal part of the pancreas obtained after multiorgan procurement from deceased donors, which was unsuitable for transplantation. Tissue-specific matrix was obtained by a combination of physical and chemical methods of pancreatic decellularization. A freeze-thaw cycle protocol and two protocols using osmotic shock were used. Samples of initial pancreatic tissue and decellularized fragments were subjected to histological analysis.Results. It was shown that a physico-chemical method with freeze-thaw cycles is suitable for effective pancreatic decellularization in severe lipomatosis; a physico-chemical method using osmotic shock, but different protocol variants, is suitable for pancreas with diffuse fibrosis and for pancreas without pronounced signs of fibrosis and lipomatosis.Conclusion. For complete human pancreatic decellularization, the protocol should be correlated with histological features of the original tissue.

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Decellularization of donor pancreatic fragment to obtain a tissue-specific matrix scaffold

Cited by 5 publications

References 10 publications

Comparative Analysis of the Influence of Extracellular Matrix Biomimetics on the Viability and Insulin-Producing Function of Isolated Pancreatic Islets

Comparative Analysis of the Influence of Extracellular Matrix Biomimetics on the Viability and Insulin-Producing Function of Isolated Pancreatic Islets

Culture Systems of Isolated Pancreatic Islets with Extracellular Matrix Biomimetics as Tissue-Engineered Constructs of the Pancreas

Determining the optimal pancreatic decellularization protocol, taking into account tissue morphological features

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