Decellularization of organs and tissues

Startseva, O.I.; Sinelnikov, M E; Babayeva, Y.V.; Trushenkova, V. V.

doi:10.17116/hirurgia201908159

Cited by 6 publications

(2 citation statements)

References 27 publications

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“…These are the main reasons why decellularized ECM (dECM) constructs derived from different tissues or organs have become central to functional tissue reconstruction [10][11][12][13]. Today, there are many products in this category that are approved by the FDA for clinical use [14]. Moreover, their intrinsic tissue-specific cues hold great promise as an inductive template to aid in the constructive remodeling of damaged tissues [15].…”

Section: Introductionmentioning

confidence: 99%

Magnetic biocomposite scaffold based on decellularized tendon ECM and MNP-deposited halloysite nanotubes: physicochemical, thermal, rheological, mechanical and in vitro biological evaluations

Koç-Demir,

Elçin,

Elçin

2024

Biomed. Mater.

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The development of new three-dimensional (3D) biomaterials with advanced versatile properties is critical to the success of tissue engineering (TE) applications. Here, (a) bioactive decellularized tendon extracellular matrix (dECM) with a sol-gel transition feature at physiological temperature, (b) halloysite nanotubes (HNT) with known mechanical properties and bioactivity, and (c) magnetic nanoparticles (MNP) with superparamagnetic and osteogenic properties were combined to develop a new scaffold that could be used in prospective bone TE applications. Deposition of MNPs on HNTs resulted in magnetic nanostructures without agglomeration of MNPs. A completely cell-free, collagen- and glycosaminoglycan-rich dECM was obtained and characterized. dECM-based scaffolds incorporated with 1%, 2% and 4% MNP-HNT were analysed for their physical, chemical, and in vitro biological properties. FTIR, XRD and VSM analyses confirmed the presence of dECM, HNT and MNP in all scaffold types. The capacity to form apatite layer upon incubation in simulated body fluid revealed that dECM-MNP-HNT is a bioactive material. Combining dECM with MNP-HNT improved the thermal stability and compressive strength of the macroporous scaffolds upto 2% MNP-HNT. In vitro cytotoxicity and hemolysis experiments showed that the scaffolds were essentially biocompatible. Human bone marrow mesenchymal stem cells (BM-MSCs) adhered and proliferated well on the macroporous constructs containing 1% and 2% MNP-HNT; and remained metabolically active for at least 21 days in vitro. Collectively, the findings support the idea that magnetic nanocomposite dECM scaffolds containing MNP-HNT could be a potential template for BTE applications.

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Section: Introductionmentioning

confidence: 99%

Magnetic biocomposite scaffold based on decellularized tendon ECM and MNP-deposited halloysite nanotubes: physicochemical, thermal, rheological, mechanical and in vitro biological evaluations

Koç-Demir,

Elçin,

Elçin

2024

Biomed. Mater.

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“…[ 7 ] However, the cell‐residued SIS could trigger a potential detrimental immune reaction. [ 8 ] Thus, the process of decellularizing SIS is crucial in order to eliminate any potential adverse effects. A successful decellularization of tissues requires the use of both physical and chemical or enzymatic methods.…”

Section: Introductionmentioning

confidence: 99%

Hydroxyapatite‐Coated Small Intestinal Submucosa Membranes Enhanced Periodontal Tissue Regeneration through Immunomodulation and Osteogenesis via BMP‐2/Smad Signaling Pathway

Cheng,

Guo,

et al. 2023

Adv Healthcare Materials

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Periodontitis, a chronic infection causing periodontal tissue loss, may be effectively addressed with in situ tissue engineering. Small intestinal submucosa (SIS) offers exceptional biocompatibility and biodegradability but lacks sufficient osteoconductive and osteoinductive properties. This study develops and characterizes SIS coated with hydroxyapatite (SIS‐HA) and gelatin methacrylate hydroxyapatite (SIS‐Gel‐HA) using biomineralization and chemical crosslinking. The impact on periodontal tissue regeneration is assessed by evaluating macrophage immune response and osteogenic differentiation potential of periodontal ligament stem cells (PDLSCs) in vitro and rat periodontal defects in vivo. The jejunum segment, with the highest collagen type I content, is optimal for SIS preparation. SIS retains collagen fiber structure and bioactive factors. Calcium content is 2.21% in SIS‐HA and 2.45% in SIS‐Gel‐HA, with no significant differences in hydrophilicity, physicochemical properties, protein composition, or biocompatibility among SIS, SIS‐HA, SIS‐Gel, and SIS‐Gel‐HA. SIS has been found to upregulate M2 marker expression, both SIS‐HA and SIS‐Gel‐HA enhance the osteogenic differentiation of PDLSCs through the BMP‐2/Smad signaling pathway, and SIS‐HA demonstrates superior in vitro osteogenic activity. In vivo, SIS‐HA and SIS‐Gel‐HA yield denser, more mature bones with the highest BMP‐2 and Smad expression. SIS‐HA and SIS‐Gel‐HA demonstrate enhanced immunity‐osteogenesis coupling, representing a promising periodontal tissue regeneration approach.This article is protected by copyright. All rights reserved

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Applications of Decellularized Extracellular Matrix for Regenerative Medicine

Chai

2021

Engineering Materials for Stem Cell Regeneration

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Decellularization of organs and tissues

Cited by 6 publications

References 27 publications

Magnetic biocomposite scaffold based on decellularized tendon ECM and MNP-deposited halloysite nanotubes: physicochemical, thermal, rheological, mechanical and in vitro biological evaluations

Magnetic biocomposite scaffold based on decellularized tendon ECM and MNP-deposited halloysite nanotubes: physicochemical, thermal, rheological, mechanical and in vitro biological evaluations

Hydroxyapatite‐Coated Small Intestinal Submucosa Membranes Enhanced Periodontal Tissue Regeneration through Immunomodulation and Osteogenesis via BMP‐2/Smad Signaling Pathway

Applications of Decellularized Extracellular Matrix for Regenerative Medicine

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