Decellularization protocols of porcine heart valves differ importantly in efficiency of cell removal and susceptibility of the matrix to recellularization with human vascular cells

Rieder, Erwin; Kasimir, Marie-Theres; Silberhumer, Gerd R.; Seebacher, Gernot; Wolner, Ernst; Simon, Paul; Weigel, Guenter

doi:10.1016/j.jtcvs.2003.06.017

Cited by 370 publications

(291 citation statements)

References 24 publications

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“…Many organs, e.g. urinary bladder [6], small intestinal [7][8][9][10][11], heart valves [12][13][14][15], skin [16,17], liver [18], anterior crucial ligament bone [19], kidney [20], pericardium [21], blood vessels [22], nerves [23], skeletal muscle [24], tendon [25] and vocal cord [26,27] from bovine and porcine tissues, have been used for fabricating the ECMbased scaffold. But, the use of goat-lung has not been focused for tissue engineering applications.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity and composition of decellularized goat lung extracellular matrix for its tissue engineering applications

Gupta¹,

Dinda²,

Mishra³

2017

Biol Eng Med

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In this study, scaffold was fabricated from goat-lung of cadaver goat by decellularization method for its potential application in tissue engineering. The cellular components of goat-lung were removed by employing trypsin-SDS based method of decellularization. DNA quantification and DAPI staining verified the effective removal of native cells from the goat-lung. Verhoeff-Van Gieson (VVG) staining, H&E staining and Periodic-Acid Schiff (PAS) staining histologically assessed the composition of native and decellularized goat-lung matrix-demonstrating the presence of collagen, elastin and glycoproteins in the decellularized goat-lung matrix. Antibacterial activity of decellularized goat-lung matrix was evaluated against gram-negative (Escherichiacoli) and gram-positive (Staphylococcusaureus) bacteria, which shows the presence of naturally occurring bioactive peptides-exhibiting the antibacterial activity against E. coli for up to 9h and against S. aureus for up to 5h. The decellularized goat-lung matrix, as demonstrated by MTT assay, was found to be biocompatibile to L929 mouse fibroblasts, HepG2, human skin derived mesenchymal stem cells (hS-MSCs), osteoblasts and BMMSCs. All the above results confer the applicability of the decellularized goat-lung matrix as a potential scaffold for tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

Antibacterial activity and composition of decellularized goat lung extracellular matrix for its tissue engineering applications

Gupta¹,

Dinda²,

Mishra³

2017

Biol Eng Med

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“…11,[14][15][16][17][18][19][20][21][22][23][24] When tissues have been efficiently decellularized, the ECM-specific structural and functional components that remain (collagen, laminin, fibronectin, growth factors, etc.) are relatively well conserved across mammalian species and, therefore, do not evoke adverse immune reaction.…”

mentioning

confidence: 99%

Comparison of Three Methods for the Derivation of a Biologic Scaffold Composed of Adipose Tissue Extracellular Matrix

Brown

Freund

Han

et al. 2011

Tissue Engineering Part C: Methods

194

197

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“…Decellularization with 0.25% Triton X-100 (Fischer Scientific, Pittsburgh, PA) and 0.25% deoxycholic acid (DOC; Alfa Aesar Ward Hill, MA) was performed using a modification of the methods reported by Rieder et al 13 Briefly, lymph nodes were digested in a solution of 0.25% Triton X/ 0.25% DOC for 24 h at 37°C. Tissues were subsequently washed in sterile PBS at 4°C for 24 h with serial changes of PBS.…”

Section: Lymph Node Harvest and Decellularizationmentioning

confidence: 99%

Decellularized Lymph Nodes as Scaffolds for Tissue Engineered Lymph Nodes

Cuzzone

Albano

Aschen

et al. 2015

Lymphatic Research and Biology

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Background: The lymphatic system is commonly injured during cancer treatment. However, despite the morbidity of these injuries, there are currently no options for replacing damaged lymphatics. The purpose of this study was to optimize methods for decellularization of murine lymph nodes (LN) and to determine if these scaffolds can be used to tissue engineer lymph node-like structures. Methods and Results: LNs were harvested from adult mice and subjected to various decellularization protocols. The degree of decellularization and removal of nuclear material was analyzed histologically and quantitatively using DNA isolation. In addition, we analyzed histological architecture by staining for matrix proteins. After the optimal method of decellularization was identified, decellularized constructs were implanted in the renal capsule of syngeneic or allogeneic recipient mice and analyzed for antigenicity. Finally, to determine if decellularized constructs could deliver lymphocytes to recipient animals, the matrices were repopulated with splenocytes, implanted in submuscular pockets, and harvested 14 days later. Decellularization was best accomplished with the detergent sodium dodecyl sulfate (SDS), resulting in negligible residual cellular material but maintenance of LN architecture. Implantation of decellularized LNs into syngeneic or allogeneic mice did not elicit a significant antigenic response. In addition, repopulation of decellularized LNs with splenocytes resulted in successful in vivo cellular delivery. Conclusions: We show, for the first time, that LNs can be successfully decellularized and that these matrices have preserved extracellular matrix architecture and the potential to deliver leukocytes in vivo. Future studies are needed to determine if tissue engineered lymph nodes maintain immunologic function.

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Decellularization protocols of porcine heart valves differ importantly in efficiency of cell removal and susceptibility of the matrix to recellularization with human vascular cells

Cited by 370 publications

References 24 publications

Antibacterial activity and composition of decellularized goat lung extracellular matrix for its tissue engineering applications

Antibacterial activity and composition of decellularized goat lung extracellular matrix for its tissue engineering applications

Comparison of Three Methods for the Derivation of a Biologic Scaffold Composed of Adipose Tissue Extracellular Matrix

Decellularized Lymph Nodes as Scaffolds for Tissue Engineered Lymph Nodes

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