Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches

Lehr, Eric J.; Rayat, Gina R.; Chiu, Brian; Churchill, Thomas A.; McGann, Locksley E.; Coe, James Y.; Ross, David B.

doi:10.1016/j.jtcvs.2010.02.060

Cited by 77 publications

(37 citation statements)

References 20 publications

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“…6 The nonimmunogenic properties of allogeneic extracellular matrix have been characterized in multiple animal models. 6,23,24 In this study, we utilized the ovine femoral artery model to evaluate completely biological, acellular TEVG made from fibrin remodeling by allogeneic fibroblasts in vitro as a potential solution for the clinical need of small-diameter arterial grafts. No successful arterial …”

Section: Discussionmentioning

confidence: 99%

Implantation of Completely Biological Engineered Grafts Following Decellularization into the Sheep Femoral Artery

Syedain

Meier

Lahti

et al. 2014

Tissue Engineering Part A

129

108

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The performance of completely biological, decellularized engineered allografts in a sheep model was evaluated to establish clinical potential of these unique arterial allografts. The 4-mm-diameter, 2-3-cm-long grafts were fabricated from fibrin gel remodeled into an aligned tissue tube in vitro by ovine dermal fibroblasts. Decellularization and subsequent storage had little effect on graft properties, with burst pressure exceeding 4000 mmHg and the same compliance as the ovine femoral artery. Grafts were implanted interpositionally in the femoral artery of six sheep (n = 9), with contralateral sham controls (n = 3). At 8 weeks (n = 5) and 24 weeks (n = 4), all grafts were patent and showed no evidence of dilatation or mineralization. Mid-graft lumen diameter was unchanged. Extensive recellularization occurred, with most cells expressing aSMA. Endothelialization was complete by 24 weeks with elastin deposition evident. These completely biological grafts possessed circumferential alignment/mechanical anisotropy characteristic of native arteries and were cultured only 5 weeks prior to decellularization and storage as ''off-the-shelf'' grafts.

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Section: Discussionmentioning

confidence: 99%

Implantation of Completely Biological Engineered Grafts Following Decellularization into the Sheep Femoral Artery

Syedain

Meier

Lahti

et al. 2014

Tissue Engineering Part A

129

108

View full text Add to dashboard Cite

show abstract

“…However, this approach has been met with limited success in preclinical and clinical studies with primary failure modes of calcification and occlusion. [35][36][37] In the case of myocardial tissue repair, an organ-specific cardiac ECM is thought to provide the most appropriate platform for a cardiac patch material. Singelyn et al have recently developed an injectable form of C-ECM with the ability to support cardiac specific cells within the matrix after injection.…”

Section: -34mentioning

confidence: 99%

Urinary bladder matrix promotes site appropriate tissue formation following right ventricle outflow tract repair

et al. 2013

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“…These chemical's compositions destruct cell connections to collagen and fibronectin by separating ions such as Ca 2+ and Mg 2 (97-100). Chelating agents are usually used in combination with enzymes such as trypsin (9,52,(101)(102)(103) or detergents (9,101) and do not have any application on its own (104). EDTA in the long term reduces the mechanical properties of the scaffold (105).…”

Section: Chelatesmentioning

confidence: 99%

Chemical Decellularization Methods and Its Effects on Extracellular Matrix

Zahmati

Alipoor

Shahmirzadi

et al. 2017

IMMINV

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Background: Extracellular matrix (ECM) produced by tissue decellularization processes as a biological scaffold due to its unique properties compared to other scaffolds for migration and implantation of stem cells have been used successfully in the field of tissue engineering and regenerative medicine in the last years. The objective of this manuscript was to provide an overview of the chemical decellularization methods, evaluation of decellularized ECM and the potential effect of the chemical decellularization agents on the biochemical composition. Methods: We searched in Google Scholar, PubMed, Scopus, and Science Direct. The literature search was done by using the following keywords: "ECM, biologic scaffold, decellularization, chemical methods, tissue engineering." We selected articles have been published from 2000 to 2016, and 15 full texts and 97 abstracts were reviewed. Results: Employing an optimization method to minimize damage to the ECM ultrastructure as for a result of the lack of reduction in mechanical properties and also the preservation of essential proteins such as laminin, fibronectin, Glycosaminoglycans (GAGs), growth factor is required. Various methods include chemical, physical and enzymatic technics were studied. However, on each of these methods can have undesirable effects on ECM. Conclusion: It is suggested that instead of the Sodium dodecyl sulfate (SDS) which have high strength degradation, we can use zwitterionic separately or in combination with SDS. Tributyl phosphate (TBP) due to its unique properties can be used in decellularization process.

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Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches

Abstract: Decellularization of allograft vascular tissue minimized the recipient cellular immune response and eliminated the production of anti-donor antibodies in recipients.

Cited by 77 publications

References 20 publications

Implantation of Completely Biological Engineered Grafts Following Decellularization into the Sheep Femoral Artery

Implantation of Completely Biological Engineered Grafts Following Decellularization into the Sheep Femoral Artery

Urinary bladder matrix promotes site appropriate tissue formation following right ventricle outflow tract repair

Chemical Decellularization Methods and Its Effects on Extracellular Matrix

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