Decellularization Techniques for Tissue Engineering

Abstract

Extracellular matrix (ECM) scaffolds have been broadly used in tissue engineering because of their versatile bioactive nature. The ECM can be derived from various tissues, organs, and cultured cells. A variety of decellularization methods have been developed to maximize the decellularization effect while minimize the effect on ECM. They include chemical, biological, and physical methods and their combinations. The properties and applications of ECM are dependent on decellularization methods. This article summarizes the decellularization methods for preparation of acellular ECM scaffolds.