Decellularized adipose tissue: A key factor in promoting fat regeneration by recruiting and inducing mesenchymal stem cells

Jiang, Xiao; Lai, Xinrui; Lu, Jin-Qiang; Tang, Ling‐Zhi; Zhang, Jinrong; Liu, Hongwei

doi:10.1016/j.bbrc.2020.12.108

Cited by 15 publications

(8 citation statements)

References 35 publications

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“…Freeze-thaw is used as a precursor step to decellularization and cannot effectively decellularize any kind of tissue alone. 128 , 129 Therefore, freeze-thaw cycles have been followed by washes with detergents such as Triton X-100, 130 – 132 solvents like isopropanol, 133 and enzymes such as trypsin. 58 This method assists in retaining a majority of the ECM’s 3D structural integrity and allowing for reduced exposure required for adequate cell removal by chemical or enzymatic agents.…”

Section: Physical/mechanical Decellularizationmentioning

confidence: 99%

Decellularization for the retention of tissue niches

Moffat

Jin

2022

J Tissue Eng

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Decellularization of natural tissues to produce extracellular matrix is a promising method for three-dimensional scaffolding and for understanding microenvironment of the tissue of interest. Due to the lack of a universal standard protocol for tissue decellularization, recent investigations seek to develop novel methods for whole or partial organ decellularization capable of supporting cell differentiation and implantation towards appropriate tissue regeneration. This review provides a comprehensive and updated perspective on the most recent advances in decellularization strategies for a variety of organs and tissues, highlighting techniques of chemical, physical, biological, enzymatic, or combinative-based methods to remove cellular contents from tissues. In addition, the review presents modernized approaches for improving standard decellularization protocols for numerous organ types.

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Section: Physical/mechanical Decellularizationmentioning

confidence: 99%

Decellularization for the retention of tissue niches

Moffat

Jin

2022

J Tissue Eng

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“…After bleeding stops, wound healing needs four stages including inflammation, mature epithelial cell proliferation, organization, and inflammation. As one of the most important key points in this stage, if the inflammatory response cannot be controlled effectively and timely, it will cause the wound inside as the cell activity increases, induce a large number of proinflammatory factors, and inhibit tissue repair [ 11 ]. However, DAT combined with the VSD technique in the combined group could not only keep the wound surface clean and wet but also avoid the contamination of DAT dry junctions, maintain the biological activity of DAT during the treatment, improve the survival rate of fibroblasts, and further promote wound healing [ 12 ].…”

Section: Related Workmentioning

confidence: 99%

[Retracted] Analysis of DAT Combined with the VSD Technique in Wound Repair of Rats and Its Effect on Inflammatory Factors

X¹,

Xu²,

Liu³

et al. 2022

Contrast Media & Molecular Imaging

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The clinical efficacy of decellularized adipose tissue (DAT) combined with vacuum sealing drainage (VSD) in the treatment of wound healing in rats is investigated, and the changes of inflammatory factors are analyzed. The tissue defect model of SD (Sprague-Dawley) rats is established and divided into the combined group (n = 12) and the control group (n = 12) according to different treatment methods. The control group is treated with a single VSD technique, and the combined group is treated with DAT on the basis of the control group. The wound healing time of the two groups is observed. Wound tissue is collected 1 day, 10 days, 20 days, and 30 days after treatment, and neutrophil infiltration is observed by HE (hematoxylin-eosin) staining. The expression changes of IL-6 and IL-13 at each time point before and after treatment are compared. Histological observation shows that the cell infiltration is reduced in both groups, and the wound repair in the combined group is better than that in the control group. The experimental results show that the DAT combined with the VSD technique can further speed up wound healing and reduce inflammation in rats.

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“…The metabolic processes of lipid storage and lipolysis of adipocyte require high oxygen consumption 53 . Hence, the rich vascular network within the adipose tissue and the degree of re-vascularization after autologous fat grafting has an important impact on volume retention 42 , 54 . The DAT contains more cytokines that promote adipose tissue formation and is more conducive to adipose tissue regeneration than other tissues 54 .…”

Section: Discussionmentioning

confidence: 99%

“…Hence, the rich vascular network within the adipose tissue and the degree of re-vascularization after autologous fat grafting has an important impact on volume retention 42 , 54 . The DAT contains more cytokines that promote adipose tissue formation and is more conducive to adipose tissue regeneration than other tissues 54 . Histological staining such as HE, Masson, and Oil Red O staining showed that the DAT lost the inherent adipocytes and vascular structures of adipose tissue, retaining only the loosely arranged extracellular matrix and luminal structures.…”

Section: Discussionmentioning

confidence: 99%

Engineered nanovesicles from stromal vascular fraction promote angiogenesis and adipogenesis inside decellularized adipose tissue through encapsulating growth factors

Tang

Zeng

et al. 2023

Sci Rep

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Acellular matrix is a commonly used biomaterial in the field of biomedical engineering and revascularization is the key process to affect the effect of acellular matrix on tissue regeneration. The application of bioactive factors related to angiogenesis has been popular in the regulation of revascularization, but the immune system clearance, uncontrollable systemic reactions, and other factors make this method face challenges. Recent reports showed that engineered cells into nanovesicles can reorganize cell membranes and encapsulate cellular active factors, extending the in vitro preservation of cytokines. However, the problems of exogenous biological contamination and tumorigenicity restricted the clinical transformation and wide application of this method. Here, we for the first time engineer stromal vascular fraction (SVF) which is extracted from fat into nanovesicles (SVF-EVs) for angiogenesis in the acellular matrix. SVF-EVs not only promote the migration of vascular endothelial cells in vitro, but also facilitate the lipogenic differentiation of mesenchymal stem cells. In vivo, SVF-EVs enhanced the retention of decellularized adipose tissue after transplanting to the subcutaneous area of nude mice. Immunofluorescence staining further showed that SVF-EVs promoted the formation of vascular networks with large lumen diameter in the grafted acellular matrix, accompanied by adipocyte regeneration peripherally. These findings reveal that SVF-EVs can be a viable method for accelerating revascularization in acellular matrix, and this process of squeezing tissue into nanovesicles shows the potential for rapid clinical transformation.

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Decellularized adipose tissue: A key factor in promoting fat regeneration by recruiting and inducing mesenchymal stem cells

Cited by 15 publications

References 35 publications

Decellularization for the retention of tissue niches

Decellularization for the retention of tissue niches

[Retracted] Analysis of DAT Combined with the VSD Technique in Wound Repair of Rats and Its Effect on Inflammatory Factors

Engineered nanovesicles from stromal vascular fraction promote angiogenesis and adipogenesis inside decellularized adipose tissue through encapsulating growth factors

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