Decellularized breast matrix as bioactive microenvironment for in vitro three‐dimensional cancer culture

Abstract

Breast cancer (BC) is a leading cause of cancer-related death in women with unsatisfactory survival rates. Advances in the understanding of the genetic basis of BC provide the opportunity to develop gene-based medicines capable of treating metastatic diseases. Here, we first demonstrated efficient tissue engineering approaches applied to normal breast and BC extracellular matrix (ECM) starting from decellularized human biopsies to generate a three-dimensional (3D) bioactive model with the sodium lauryl ether sulfate solution. The decellularized tissues maximized the genetic component removal from tissues and minimally injured ECM structures and native compositions by histology and ECM compositions analyses.Importantly, we proved that the 3D ECM retained tissues biological properties. We demonstrated that after 30 days of recellularization with MCF-7 cell (human breast adenocarcinoma cell line), the 3D cancer ECM induced an overexpression of epithelial-mesenchymal transition (EMT) and cancer proliferation. Meanwhile, normal ECM from the breast inhibited EMT and cell growth with the inducement of apoptosis. Given the biological activity preserved in the ECM after decellularization, we believe these approaches are powerful tools for future preclinical research for BC and breast development.

Human breast cancer decellularized scaffolds promote epithelial‐to‐mesenchymal transitions and stemness of breast cancer cells in vitro

“…And we hypothesized that it may be suitable for human cancer tissues. In our previous studies, we confirmed that the decellularized scaffold with SDS solution from human breast cancer tissues would promote the cell migration and proliferation in vitro (Qin. et al, 2018).…”

Antioxidant and Antiproliferative Activities of Cyanidin‐3‐O‐Glucoside (C3G) Liposome in Caco‐2 Cells Cultivated in 2D and 3D Cell Culture Models

“…But, the 2D conventional cell culture systems has been questioned because of potential changes in morphology and gene expression, often resulting in distortions in cell behavior and biological functions of cultured cells as compared with cells in natural organisms and tissues (Fang & Eglen, ; Huang et al., ; Jin et al., ). As for the animal model, the animals model has many uncontrollable factors such as endogenous growth factors and lack of an immune system (Jin et al., ).To better get the cell morphology, behavior and functions, the concept of 3D cell culture was proposed, that is cell growth in a 3D environment with sufficient and multidirectional cell–cell interactions mimicking the in vivo architecture of natural organs and tissues (Achilli, Meyer, & Morgan, ; Kehr, ). The 3D cell culture model is providing new insights into tumor biology and also in differentiation, tissue organization, and homeostasis (Kelm, Timmins, Brown, Fussenegger, & Nielsen, ).…”

Decellularized Extracellular Matrix for Cancer Research

“…Comparative studies of cell behavior in normal and cancerous tissue-derived dECM were also performed to determine the roles of cancerous ECM. Jin et al compared the behavior of the MCF-7 cell line in dECM that was derived from normal and cancerous breast tissues [68]. They demonstrated that the proliferation of MCF-7 cells was decreased in cancerous dECM compared with normal dECM.…”