Decellularized ECM-derived bioinks: Prospects for the future

Kabirian, Fatemeh; Mozafari, Masoud

doi:10.1016/j.ymeth.2019.04.019

Cited by 137 publications

(131 citation statements)

References 89 publications

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“…Although medical devices are commercially available to be impregnated with SNAP molecules, their fabrication based on patients' individual geometry of the pathologic site is advantageous. 3D printing is a layer by layer additive manufacturing process which can create grafts according to the designed 3D CAD model (Kabirian & Mozafari, ). In the current study, fused deposition modeling (FDM) was used as a simple 3D printing method which creates the constructs by extruding and solidifying the polymer according to the CAD model.…”

Section: Introductionmentioning

confidence: 99%

Additively manufactured small‐diameter vascular grafts with improved tissue healing using a novel SNAP impregnation method

et al. 2019

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The vascular network has a complex architecture such as branches, curvatures, and bifurcations which is even more complicated in view of individual patients' defect anatomy requiring custom‐specifically designed vascular implants. In this work, 3D printing is used to overcome these challenges and a new shorter impregnation method was developed to incorporate S‐nitroso‐N‐acetyl‐d‐penicillamine (SNAP) as a nitric oxide (NO) donor to printed grafts. The 3D‐printed small‐diameter vascular grafts (SDVGs) were impregnated with SNAP solution during SNAP synthesis (S1) or with SNAP dissolved in methanol (S2). The advantage of the newly developed S1 impregnation method is the elimination of the synthesis step by direct impregnation inside the S1 solution. Scanning electron microscopy imaging reveals the successful crystal formation in both methods. The results demonstrate that both S1‐ and S2‐impregnated grafts, after covering with polycaprolactone topcoat, can release NO in a controlled manner and in the physiological range (0.5–4.0 × 10−10 mol cm−2 min−1) over a 15 days period. The created grafts with a NO‐releasing surface have also shown bactericidal effect while the healing properties of the implant were improved by promoting migration and proliferation of endothelial cells (ECs). These results suggest that incorporation of 3D printing technology with the newly developed S1 impregnation of SNAP can optimize and shorten the manufacturing process of the next generation of patient‐based antibacterial SDVGs with a higher attraction for ECs.

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Section: Introductionmentioning

confidence: 99%

Additively manufactured small‐diameter vascular grafts with improved tissue healing using a novel SNAP impregnation method

et al. 2019

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“…Triton X-100 is known to decrease the amount of fibronectin and laminin in the final dECM. Triton-X-100 is known to damage the ultrastructure of the ECM in addition to removal of some glycosaminoglycans (GAGs) [62,66]. Triton X-100 was shown to leave large quantities of cellular material when used for decellularizing the tendon [65,67,68].…”

Section: Decellularization Methodsmentioning

confidence: 99%

Trends in Decellularized Extracellular Matrix Bioinks for 3D Printing: Inspiring a new Kind of Medicine

Dzobo¹,

Motaung²,

Adesida³

2019

Preprint

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The promise of regenerative medicine and tissue engineering is founded on the ability to regenerate diseased or damaged tissues and organs into functional tissues and organs or the creation of new tissues and organs altogether. In theory, all damaged and diseased tissues and organs can be regenerated or created using different configurations and combinations of extracellular matrix, cells and inductive biomolecules. Currently, regenerative medicine and tissue engineering can allow the improvement of patients’ quality of life through availing novel treatment options. Tissues and organs have a specific ECM, with specific proteins and factors released by cells residing within the local microenvironment. The coupling of regenerative medicine and tissue engineering field with 3D printing is revolutionizing the treatment of patients in a huge way. 3D bioprinting allows the proper placement of cells and ECMs, allowing the recapitulation of native microenvironments of tissues and organs. 3D bioprinting utilizes different bioinks made up of different formulations of ECM/biomaterials, biomolecules and even cells. The choice of the bioink used during 3D bioprinting is very important as properties such as printability, compatibility and physical strength influence the final construct printed. The extracellular matrix (ECM) provides both physical and mechanical microenvironment needed by cells to survive and proliferate. Decellularized ECM bioink contains biochemical cues from the original native ECM and also the right proportions of ECM proteins. Different techniques and characterization methods are used to derive bioinks from several tissues and organs and to evaluate their quality. This review discusses the uses of decellularized ECM bioinks and argues that they represent the most biomimetic bioinks available. In addition, we briefly discuss some polymer-based bioinks utilized in 3D bioprinting.

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“…Thus, dealing with bioinks made of only dECM, providing cells with their natural microenvironment, could represent a further step towards the design of a real tissue-mimicking scaffold. In this context, dECM bioinks have been derived from different organs [ 17 , 20 , 21 ]. The obtainment of dECM-based bioinks for 3D printing involves different steps [ 17 , 22 , 23 ].…”

Section: Moving Towards Biomimetic Engineered Muscular Constructsmentioning

confidence: 99%

“…The first is the decellularization of the tissue of interest by removing the cells while preserving the ECM. Several physical, chemical, enzymatic and biological processes can be adopted; the selection depends on several factors, such as tissue and organ thickness, density and lipid content [ 20 , 21 ]. To prepare a printable material, dECM needs to be processed as a hydrogel: ECM is usually crushed into a powder form and solubilized in a physiological buffer solution (generally, enzymatic acidic digestion with a pH adjusted to accommodate cells, if to be included in the final formulation) in order to deal with a solution at 10 °C and a gel at 37 °C.…”

Section: Moving Towards Biomimetic Engineered Muscular Constructsmentioning

confidence: 99%

Information-Driven Design as a Potential Approach for 3D Printing of Skeletal Muscle Biomimetic Scaffolds

Baiguera

Gaudio²,

Carotenuto

et al. 2020

Nanomaterials

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Severe muscle injuries are a real clinical issue that still needs to be successfully addressed. Tissue engineering can represent a potential approach for this aim, but effective healing solutions have not been developed yet. In this regard, novel experimental protocols tailored to a biomimetic approach can thus be defined by properly systematizing the findings acquired so far in the biomaterials and scaffold manufacturing fields. In order to plan a more comprehensive strategy, the extracellular matrix (ECM), with its properties stimulating neomyogenesis and vascularization, should be considered as a valuable biomaterial to be used to fabricate the tissue-specific three-dimensional structure of interest. The skeletal muscle decellularized ECM can be processed and printed, e.g., by means of stereolithography, to prepare bioactive and biomimetic 3D scaffolds, including both biochemical and topographical features specifically oriented to skeletal muscle regenerative applications. This paper aims to focus on the skeletal muscle tissue engineering sector, suggesting a possible approach to develop instructive scaffolds for a guided healing process.

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Decellularized ECM-derived bioinks: Prospects for the future

Cited by 137 publications

References 89 publications

Additively manufactured small‐diameter vascular grafts with improved tissue healing using a novel SNAP impregnation method

Additively manufactured small‐diameter vascular grafts with improved tissue healing using a novel SNAP impregnation method

Trends in Decellularized Extracellular Matrix Bioinks for 3D Printing: Inspiring a new Kind of Medicine

Information-Driven Design as a Potential Approach for 3D Printing of Skeletal Muscle Biomimetic Scaffolds

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