Decellularized ECM-Derived Hydrogels: Modification and Properties

Claudio‐Rizo, Jesús A.; Delgado, Jorge; Quintero-Ortega, Iraís A.; Mata-Mata, JoséL.; Mendoza-Novelo, Birzabith

doi:10.5772/intechopen.78331

Cited by 20 publications

(16 citation statements)

References 90 publications

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“…In most soft tissues, collagen provides the essential physiological elasticity that retains the tensile strength and sGAG that provide the viscoelasticity of the material. Additionally, collagen and sGAG are two of the most crucial components which directly impact the gelation kinetics and rheological properties of ECM hydrogels [ 10 , 36 ]. The retention of collagen and sGAG content after decellularization was therefore studied.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Porcine Urinary Bladder Matrix Hydrogels from Sodium Dodecyl Sulfate Decellularization Method

2020

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Urinary bladder matrix (UBM) is one of the most studied extracellular matrixes (ECM) in the tissue engineering field. Although almost all of the UBM hydrogels were prepared by using peracetic acid (PAA), recent studies indicated that PAA was not a trustworthy way to decellularize UBM. A stronger detergent, such as sodium dodecyl sulfate (SDS), may help tackle this issue; however, its effects on the hydrogels’ characteristics remain unknown. Therefore, the objective of this study was to develop a more reliable protocol to decellularize UBM, using SDS, and to compare the characteristics of hydrogels obtained from this method to the widely employed technique, using PAA. The results indicated that SDS was superior to PAA in decellularization efficacy. Different decellularization methods led to dissimilar gelation kinetics; however, the methods did not affect other hydrogel characteristics in terms of biochemical composition, surface morphology and rheological properties. The SDS-treated hydrogels possessed excellent cytocompatibility in vitro. These results showed that the SDS decellularization method could offer a more stable and safer way to obtain acellular UBM, due to reducing immunogenicity. The hydrogels prepared from this technique had comparable characteristics as those from PAA and could be a potential candidate as a scaffold for tissue remodeling.

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Section: Discussionmentioning

confidence: 99%

Characterization of Porcine Urinary Bladder Matrix Hydrogels from Sodium Dodecyl Sulfate Decellularization Method

2020

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“…Moreover, collagen type I in ECM is ideally polymerized at body temperature (37 °C) and neutral pH (7.4) [ 17 , 19 ]. Based on this phenomenon, the ADSC ECM and genipin cross-linked ADSC ECM formed gels from 4 °C to 37 °C.…”

Section: Discussionmentioning

confidence: 99%

“…It is polymerized by the self-assembly process of the fibrillar structure to generate a gel at body temperature (37 °C) and neutral pH (7.4). The collagen fiber cross-linking is promoted by hydrophobic and electrostatic interactions [ 16 , 17 , 18 , 19 ]. This temperature- and pH-dependent sol–gel state of collagen in ECM allows an injectable ECM gel [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…The collagen fiber cross-linking is promoted by hydrophobic and electrostatic interactions [ 16 , 17 , 18 , 19 ]. This temperature- and pH-dependent sol–gel state of collagen in ECM allows an injectable ECM gel [ 17 ]. Furthermore, the delivery method of ECM gel with minimum invasive and uncomplicated procedures is beneficial for both patients and clinicians [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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New Insight into Natural Extracellular Matrix: Genipin Cross-Linked Adipose-Derived Stem Cell Extracellular Matrix Gel for Tissue Engineering

Nyambat

Manga

Chen

et al. 2020

IJMS

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The cell-derived extracellular matrix (ECM) is associated with a lower risk of pathogen transfer, and it possesses an ideal niche with growth factors and complex fibrillar proteins for cell attachment and growth. However, the cell-derived ECM is found to have poor biomechanical properties, and processing of cell-derived ECM into gels is scarcely studied. The gel provides platforms for three-dimensional cell culture, as well as injectable biomaterials, which could be delivered via a minimally invasive procedure. Thus, in this study, an adipose-derived stem cell (ADSC)-derived ECM gel was developed and cross-linked by genipin to address the aforementioned issue. The genipin cross-linked ADSC ECM gel was fabricated via several steps, including rabbit ADSC culture, cell sheets, decellularization, freeze–thawing, enzymatic digestion, neutralization of pH, and cross-linking. The physicochemical characteristics and cytocompatibility of the gel were evaluated. The results demonstrated that the genipin cross-linking could significantly enhance the mechanical properties of the ADSC ECM gel. Furthermore, the ADSC ECM was found to contain collagen, fibronectin, biglycan, and transforming growth factor (TGF)-β1, which could substantially maintain ADSC, skin, and ligament fibroblast cell proliferation. This cell-derived natural material could be suitable for future regenerative medicine and tissue engineering application.

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“…In this sense, collagen hydrogels with tailored mechanical properties are required, allowing the coupling of molecules and/or polymers inside the 3D matrix, generating systems with controlled degradation rate and bacterial inhibition capacity. 1,6,7 Various strategies to improve the properties of collagen hydrogels have been reported. Physical cross-linking methods using exogenous components generate hybrid matrices with adapted mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

Novel semi‐interpenetrated networks based on collagen‐polyurethane‐polysaccharides in hydrogel state for biomedical applications

Claudio‐Rizo

Hernandez‐Hernandez

Cano-Salazar

et al. 2020

J of Applied Polymer Sci

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The development of collagen hydrogels with tailored properties for improved applications in biomedicine represents an area of opportunity for materials science. The collagen can form semi-interpenetrated networks (semi-IPN) with various natural and/or synthetic polymers. This work aims the preparation of novel hydrogels generated from a collagen matrix cross-linked with polyurethane (PU), and the subsequent inclusion of polysaccharide chains to form semi-IPN systems with improved properties. The choice of polysaccharides for this purpose is related to their ability to modulate the biocompatibility and the antibacterial capacity in various biomedical strategies. The work

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Decellularized ECM-Derived Hydrogels: Modification and Properties

Cited by 20 publications

References 90 publications

Characterization of Porcine Urinary Bladder Matrix Hydrogels from Sodium Dodecyl Sulfate Decellularization Method

Characterization of Porcine Urinary Bladder Matrix Hydrogels from Sodium Dodecyl Sulfate Decellularization Method

New Insight into Natural Extracellular Matrix: Genipin Cross-Linked Adipose-Derived Stem Cell Extracellular Matrix Gel for Tissue Engineering

Novel semi‐interpenetrated networks based on collagen‐polyurethane‐polysaccharides in hydrogel state for biomedical applications

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