Decellularized Extracellular Matrices and Cardiac Differentiation: Study on Human Amniotic Fluid-Stem Cells

Gaggi, Giulia; Credico, Andrea Di; Izzicupo, Pascal; Sancilio, Silvia; Mauro, Michele Di; Iannetti, Gian Domenico; Dolci, Susanna; Amabile, Giovanni; Baldassarre, Angela Di; Ghinassi, Barbara

doi:10.3390/ijms21176317

Cited by 13 publications

(12 citation statements)

References 56 publications

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“…In recent years, the advancements in human stem cell technology allowed the in vitro generation of different cell type characterized electrical activity such as cardiomyocytes and neuronal cells [ 26 , 27 , 28 ]; in particular, by means of specific differentiation protocols various neuronal cell types have been derived from iPSC [ 29 , 30 ], and recently also from perinatal stem cells [ 31 , 32 , 33 ]. The electrical properties of these neuronal cells can be analyzed by MEAs, a technology that allows one to record spontaneous electrical activity for long time periods, in a non-invasive manner.…”

Section: Discussionmentioning

confidence: 99%

Real-Time Monitoring of Levetiracetam Effect on the Electrophysiology of an Heterogenous Human iPSC-Derived Neuronal Cell Culture Using Microelectrode Array Technology

et al. 2021

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Levetiracetam (LEV) is a broad-spectrum and widely used antiepileptic drug that also has neuroprotective effects in different neurological conditions. Given its complex interaction with neuronal physiology, a better comprehension of LEV effects on neurons activity is needed. Microelectrode arrays (MEAs) represent an advanced technology for the non-invasive study of electrophysiological activity of neuronal cell cultures. In this study, we exploited the Maestro Edge MEA system, a platform that allows a deep analysis of the electrical network behavior, to study the electrophysiological effect of LEV on a mixed population of human neurons (glutamatergic, GABAergic and dopaminergic neurons, and astrocytes). We found that LEV significantly affected different variables such as spiking, single-electrode bursting, and network bursting activity, with a pronounced effect after 15 min. Moreover, neuronal cell culture completely rescued its baseline activity after 24 h without LEV. In summary, MEA technology confirmed its high sensitivity in detecting drug-induced electrophysiological modifications. Moreover, our results allow one to extend the knowledge on the electrophysiological effects of LEV on the complex neuronal population that resembles the human cortex.

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Section: Discussionmentioning

confidence: 99%

Real-Time Monitoring of Levetiracetam Effect on the Electrophysiology of an Heterogenous Human iPSC-Derived Neuronal Cell Culture Using Microelectrode Array Technology

et al. 2021

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“…Mid-trimester human amniotic fluid cells are a heterogeneous population that includes stem cells with intermediate phenotype between the pluripotent and the adult stem cells: indeed, they are characterized by a high proliferation rate, the expression of both pluripotent and mesenchymal markers while maintaining the non-tumor-forming properties of adult cells. All these characteristics encourage the study of their use in regenerative medicine (Gaggi et al, 2020c). As Amniotic Fluids contain a heterogeneous mixture of cell types, the phenotyping of the amniotic cell samples is essential to characterize the starting populations in differentiation experiments.…”

Section: Discussionmentioning

confidence: 99%

Human mesenchymal amniotic fluid stem cells reveal an unexpected neuronal potential differentiating into functional spinal motor neurons

Gaggi

Credico

Guarnieri

et al. 2022

Front. Cell Dev. Biol.

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Human amniotic fluids stem cells (hAFSCs) can be easily isolated from the amniotic fluid during routinely scheduled amniocentesis. Unlike hiPSCs or hESC, they are neither tumorigenic nor immunogenic and their use does not rise ethical or safety issues: for these reasons they may represent a good candidate for the regenerative medicine. hAFSCs are generally considered multipotent and committed towards the mesodermal lineages; however, they express many pluripotent markers and share some epigenetic features with hiPSCs. Hence, we hypothesized that hAFSCs may overcome their mesodermal commitment differentiating into to ectodermal lineages. Here we demonstrated that by the sequential exposure to specific factors, hAFSCs can give rise to spinal motor neurons (MNs), as evidenced by the gradual gene and protein upregulation of early and late MN markers (PAX6, ISL1, HB9, NF-L, vAChT). When co-cultured with myotubes, hAFSCs-derived MNs were able to create functional neuromuscular junctions that induced robust skeletal muscle contractions. These data demonstrated the hAFSCs are not restricted to mesodermal commitment and can generate functional MNs thus outlining an ethically acceptable strategy for the study and treatment of the neurodegenerative diseases.

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“…Similar approaches are available for pancreas [ 133 ] and cartilage [ 134 ]. Other relevant models based on decellularized matrices concern bone [ 135 ], spinal cord [ 136 ], kidney [ 137 ], cancers [ 138 , 139 ] and also commercially available CorTM PATCH [ 140 ] and equine Matrix PatchTM [ 140 ] currently used for in vitro studies, surgery and repair of cardiac tissue.…”

Section: Nanostructured Biomaterialsmentioning

confidence: 99%

Advanced Multi-Dimensional Cellular Models as Emerging Reality to Reproduce In Vitro the Human Body Complexity

Bassi

Grimaudo

Panseri

et al. 2021

IJMS

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A hot topic in biomedical science is the implementation of more predictive in vitro models of human tissues to significantly improve the knowledge of physiological or pathological process, drugs discovery and screening. Bidimensional (2D) culture systems still represent good high-throughput options for basic research. Unfortunately, these systems are not able to recapitulate the in vivo three-dimensional (3D) environment of native tissues, resulting in a poor in vitro–in vivo translation. In addition, intra-species differences limited the use of animal data for predicting human responses, increasing in vivo preclinical failures and ethical concerns. Dealing with these challenges, in vitro 3D technological approaches were recently bioengineered as promising platforms able to closely capture the complexity of in vivo normal/pathological tissues. Potentially, such systems could resemble tissue-specific extracellular matrix (ECM), cell–cell and cell–ECM interactions and specific cell biological responses to mechanical and physical/chemical properties of the matrix. In this context, this review presents the state of the art of the most advanced progresses of the last years. A special attention to the emerging technologies for the development of human 3D disease-relevant and physiological models, varying from cell self-assembly (i.e., multicellular spheroids and organoids) to the use of biomaterials and microfluidic devices has been given.

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Decellularized Extracellular Matrices and Cardiac Differentiation: Study on Human Amniotic Fluid-Stem Cells

Cited by 13 publications

References 56 publications

Real-Time Monitoring of Levetiracetam Effect on the Electrophysiology of an Heterogenous Human iPSC-Derived Neuronal Cell Culture Using Microelectrode Array Technology

Real-Time Monitoring of Levetiracetam Effect on the Electrophysiology of an Heterogenous Human iPSC-Derived Neuronal Cell Culture Using Microelectrode Array Technology

Human mesenchymal amniotic fluid stem cells reveal an unexpected neuronal potential differentiating into functional spinal motor neurons

Advanced Multi-Dimensional Cellular Models as Emerging Reality to Reproduce In Vitro the Human Body Complexity

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