Decellularized tissues as platforms for in vitro modeling of healthy and diseased tissues

McCrary, Michaela W.; Bousalis, Deanna; Mobini, Sahba; Song, Young Hye; Schmidt, Christine E.

doi:10.1016/j.actbio.2020.05.031

Cited by 74 publications

(57 citation statements)

References 224 publications

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“…This approach ignores the important interactions of hepatic and immune cells with the surrounding microenvironment (including the ECM) via integrins and other ECM-receptors, excluding related signaling pathways from the equation. On the other side, studies in animal models certainly present the advantage of using a physiologically relevant model that includes the ECM and cellmatrix interactions, but they are also associated with ethical concerns, limited reliability for predicting human responses to treatments, substantial differences in anatomy, immune responses, metabolic activity, and development in respect to humans, and low reproducibility (422). Most importantly, mechanistic studies on ECM components usually utilize specific -/-animals for a target protein, obtaining an "all or nothing" effect that does not translate into the complexity of human disease in terms of timing and overall effect when in presence of other compensatory mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…3D in vitro models may have the right features to provide more reliable platforms to understand health and disease biology in a more cost-effective, scalable and reproducible way than in vivo models. 3D cultures have demonstrated to be more effective in reproducing in vivo responses and cellular behaviours, as thoroughly reviewed by McCrary and colleagues (422). Given the critical role of the ECM in healthy tissue function and disease development and progression, incorporation of the ECM in these 3D culture models is essential to create the appropriate tissue microenvironment.…”

Section: Discussionmentioning

confidence: 99%

“…The use of decellularized ECM-scaffolds in in vitro models is not exempt from limitations: limited mechanical instability and significant altered mechanical properties in comparison to the native counterpart; batch to batch variability due to variations in the decellularization process among studies, donor gender, sex, genetics, treatment, and anatomical tissue sampling; difficulties in assessing the exact protein content in different ECMs (422). Finally, most decellularization techniques, especially if not properly validated, inevitably removes some growth factors and ECM proteins from the matrix.…”

Section: Discussionmentioning

confidence: 99%

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Immunomodulatory Role of the Extracellular Matrix Within the Liver Disease Microenvironment

et al. 2020

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Chronic liver disease when accompanied by underlying fibrosis, is characterized by an accumulation of extracellular matrix (ECM) proteins and chronic inflammation. Although traditionally considered as a passive and largely architectural structure, the ECM is now being recognized as a source of potent damage-associated molecular pattern (DAMP)s with immune-active peptides and domains. In parallel, the ECM anchors a range of cytokines, chemokines and growth factors, all of which are capable of modulating immune responses. A growing body of evidence shows that ECM proteins themselves are capable of modulating immunity either directly via ligation with immune cell receptors including integrins and TLRs, or indirectly through release of immunoactive molecules such as cytokines which are stored within the ECM structure. Notably, ECM deposition and remodeling during injury and fibrosis can result in release or formation of ECM-DAMPs within the tissue, which can promote local inflammatory immune response and chemotactic immune cell recruitment and inflammation. It is well described that the ECM and immune response are interlinked and mutually participate in driving fibrosis, although their precise interactions in the context of chronic liver disease are poorly understood. This review aims to describe the known pro-/anti-inflammatory and fibrogenic properties of ECM proteins and DAMPs, with particular reference to the immunomodulatory properties of the ECM in the context of chronic liver disease. Finally, we discuss the importance of developing novel biotechnological platforms based on decellularized ECM-scaffolds, which provide opportunities to directly explore liver ECM-immune cell interactions in greater detail.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Immunomodulatory Role of the Extracellular Matrix Within the Liver Disease Microenvironment

et al. 2020

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“…An interesting approach to create highly biocompatible bio-inks is decellularization, which consists in removing the cellular content from animal and human-derived tissues. This method allows the production of tissue-specific ECM scaffolds that can more accurately recapitulate the native matrix [ 132 ]. These bio-inks have an enormous potential for in vitro modeling of neurodegenerative diseases phenotypes and for evaluating the tissues’ responses to new potential drugs [ 132 ].…”

Section: Scaffolds For Neural Diseases’ Modelingmentioning

confidence: 99%

“…This method allows the production of tissue-specific ECM scaffolds that can more accurately recapitulate the native matrix [ 132 ]. These bio-inks have an enormous potential for in vitro modeling of neurodegenerative diseases phenotypes and for evaluating the tissues’ responses to new potential drugs [ 132 ]. A potential issue is represented by the complexity of the brain tissue, such as the diverse conditions in terms of the growth factor content.…”

Section: Scaffolds For Neural Diseases’ Modelingmentioning

confidence: 99%

Advances in Tissue Engineering and Innovative Fabrication Techniques for 3-D-Structures: Translational Applications in Neurodegenerative Diseases

Rey

Barzaghini

Nardini

et al. 2020

Cells

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In the field of regenerative medicine applied to neurodegenerative diseases, one of the most important challenges is the obtainment of innovative scaffolds aimed at improving the development of new frontiers in stem-cell therapy. In recent years, additive manufacturing techniques have gained more and more relevance proving the great potential of the fabrication of precision 3-D scaffolds. In this review, recent advances in additive manufacturing techniques are presented and discussed, with an overview on stimulus-triggered approaches, such as 3-D Printing and laser-based techniques, and deposition-based approaches. Innovative 3-D bioprinting techniques, which allow the production of cell/molecule-laden scaffolds, are becoming a promising frontier in disease modelling and therapy. In this context, the specific biomaterial, stiffness, precise geometrical patterns, and structural properties are to be considered of great relevance for their subsequent translational applications. Moreover, this work reports numerous recent advances in neural diseases modelling and specifically focuses on pre-clinical and clinical translation for scaffolding technology in multiple neurodegenerative diseases.

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Let's Talk About Sex—Biological Sex Is Underreported in Biomaterial Studies

James

Guerin

Allen

2020

Adv Healthcare Materials

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Precision medicine aims to better individualize healthcare. It requires that biomaterials be designed for the physiological characteristics of a specific patient. To make this a reality, biomaterials research and development must address differences of biological sex. More specifically, biomaterials should be designed with properties optimized and appropriate for male and female patients. In analyzing research articles from seven prominent biomaterials journals, sex as a biological variable is missing from an overwhelming majority of in vitro biomaterial studies. From the survey, the reporting of the sex of primary cell cultures happened only 10.3% of the time. Contributing to this trend is that commercial vendors bias cell lines toward one sex or another by not disclosing information of cell line sex at the time of purchase; researchers do not communicate this pertinent information in published studies; and many journal policies have little to no requirements for reporting cell line characteristics. Omitting this valuable information leads to a gap in the understanding of sex‐specific cell‐biomaterial interactions and it creates a bias in research findings towards one sex or another. To curb this concerning trend and make precision biomaterials a reality will require the biomaterials field to “talk about sex” by reporting cell sex more broadly.

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Decellularized tissues as platforms for in vitro modeling of healthy and diseased tissues

Cited by 74 publications

References 224 publications

Immunomodulatory Role of the Extracellular Matrix Within the Liver Disease Microenvironment

Immunomodulatory Role of the Extracellular Matrix Within the Liver Disease Microenvironment

Advances in Tissue Engineering and Innovative Fabrication Techniques for 3-D-Structures: Translational Applications in Neurodegenerative Diseases

Let's Talk About Sex—Biological Sex Is Underreported in Biomaterial Studies

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