Decidua Parietalis Mesenchymal Stem/Stromal Cells and Their Secretome Diminish the Oncogenic Properties of MDA231 Cells In Vitro

Basmaeil, Yasser; Bahattab, Eman; Subayyil, Abdullah Al; Kulayb, Haya Bin; Alrodayyan, Maha; Abumaree, Mohamed; Khatlani, Tanvir

doi:10.3390/cells10123493

Cited by 9 publications

(13 citation statements)

References 82 publications

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“…A study by Klopp et al demonstrated that MSCs promote the growth of breast cancer cells in vivo, supporting the stimulatory effects of BMSCs on tumor growth and metastasis observed in our study [37]. In contrast, a study by Basmaeil et al shows that MSCs have antitumor properties and can suppress tumor growth, regulate cellular signaling, and induce apoptosis in MDA231 cells in vitro [38].…”

Section: Discussionsupporting

confidence: 90%

BMSC–HNC Interaction: Exploring Effects on Bone Integrity and Head and Neck Cancer Progression

Eichberger,

Froschhammer,

Schulz

et al. 2023

IJMS

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In recent research, the tumor microenvironment has been shown to attract mesenchymal stromal cells (MSCs), which is of particular interest due to its implications for cancer progression. The study focused on understanding the interaction between bone marrow-derived MSCs (BMSCs) and head and neck cancer (HNC) cells. This interaction was found to activate specific markers, notably the osteogenic marker alkaline phosphatase and the oncogene Runx2. These activations corresponded with the release of collagenase enzymes, MMP9 and MMP2. To gain insights into bone resorption related to this interaction, bovine bone slices were used, supporting the growth of “heterogeneous spheroids” that contained both BMSCs and HNC cells. Through scanning electron microscopy and energy-dispersive X-ray (EDX) analysis, it was observed that these mixed spheroids were linked to a notable increase in bone degradation and collagen fiber exposure, more so than spheroids of just BMSCs or HNC cells. Furthermore, the EDX results highlighted increased nitrogen content on bone surfaces with these mixed clusters. Overall, the findings underscore the significant role of BMSCs in tumor growth, emphasizing the need for further exploration in potential cancer treatment strategies.

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Section: Discussionsupporting

confidence: 90%

BMSC–HNC Interaction: Exploring Effects on Bone Integrity and Head and Neck Cancer Progression

Eichberger,

Froschhammer,

Schulz

et al. 2023

IJMS

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“…These data suggest that UCMSCs-CM had the potential to inhibit granulosa cell tumor cell motility which can be helpful to prevent metastasis. It is reported that suppressive effects of dental pulp stem cells and its conditioned medium on development and migration of colorectal cancer cells through MAPKinase pathway [53], Decidua parietalis mesenchymal stem/stromal cells (DPMSCs) and their secretome inhibit the invasive characteristics of MDA231 cells in vitro [54]. Our results consistent with these studies using MSCs against cancer cell migration and invasion.…”

Section: Disccusionsupporting

confidence: 90%

Human umbilical cord mesenchymal stem cells conditioned medium exerts anti-tumor effects on KGN cells in a cell density-dependent manner through activation of the Hippo pathway

Wan

Miao

Niu

et al. 2022

Preprint

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Objectives The conditioned medium from human umbilical cord mesenchymal stem cells (UCMSCs-CM) provides a new cell-free therapy for tumors due to its unique secretome. However, there are many contradictory reports about the effect of UCMSCs-CM on tumor cells. The loss of contact inhibition is a common characteristic of tumor cells. A relationship between the effect of UCMSCs-CM on tumor cells and contact inhibition in tumor cells is rarely concerned. Whether the effect of UCMSCs-CM on tumor cells is affected by cell density? Here, we explored the effect of UCMSCs-CM on KGN cell, which is an ovarian granulosa cell tumors cell line, at low or high density. Materials and Methods Growth curve and CCK8 assay were used to assess cell proliferation and viability. Scratch wound and matrigel invasion assay were implicated to detect cell motility of KGN cells. UCMSCs-CM effects on cell cycle, apoptosis and pathway-related proteins were investigated by flow cytometry, TUNEL assay, western blot and immunofluorescence analysis respectively. Results In growth curve analysis, before KGN cells proliferated into confluence, UCMSCs-CM had no effect on cell proliferation, but once the cells proliferate to contact each other, UCMSCs-CM significantly inhibited proliferation. Meanwhile, when KGN cells were implanted at high density, UCMSCs-CM could induce cell cycle arrest at G1 phase, inhibit cell migration, invasion and promote apoptosis. However, it had no similar effect on KGN cells implanted at low density. In mechanism, the UCMSCs-CM treatment activated the Hippo pathway when KGN cells were implanted at high density. Consistently, the MST1/2 inhibitor, XMU-MP-1, inhibited the activation of the Hippo pathway induced by UCMSCs-CM treatment and accordingly declined the anti-tumor effect of UCMSC-CM on KGN cells. Conclusion The effect of UCMSCs-CM on tumor cells is affected by cell density. UCMSCs-CM exerted anti-tumor effect on KGN cells by activating Hippo pathway to restore contact inhibition. Our results suggest that UCMSCs-CM is a promising therapeutic candidate for GCTs treatment.

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“…These data suggest that UCMSCs-CM had the potential to inhibit granulosa cell tumor cell motility which can be helpful to prevent metastasis. It is reported that suppressive effects of dental pulp stem cells and its conditioned medium on development and migration of colorectal cancer cells through MAPKinase pathway [ 51 ], decidua parietalis mesenchymal stem/stromal cells (DPMSCs) and their secretome inhibit the invasive characteristics of MDA231 cells in vitro [ 52 ]. Our results consistent with these studies using MSCs against cancer cell migration and invasion.…”

Section: Discussionmentioning

confidence: 99%

Human umbilical cord mesenchymal stem cells conditioned medium exerts anti-tumor effects on KGN cells in a cell density-dependent manner through activation of the Hippo pathway

et al. 2023

View full text Add to dashboard Cite

Background The conditioned medium from human umbilical cord mesenchymal stem cells (UCMSCs-CM) provides a new cell-free therapy for tumors due to its unique secretome. However, there are many contradictory reports about the effect of UCMSCs-CM on tumor cells. The loss of contact inhibition is a common characteristic of tumor cells. A relationship between the effect of UCMSCs-CM on tumor cells and contact inhibition in tumor cells is rarely concerned. Whether the effect of UCMSCs-CM on tumor cells is affected by cell density? Here, we explored the effect of UCMSCs-CM on granulosa tumor cell line (KGN) cells at low or high density. Methods Growth curve and CCK8 assay were used to assess cell proliferation and viability. Scratch wound and matrigel invasion assay were implicated to detect cell motility of KGN cells. UCMSCs-CM effects on cell cycle, apoptosis and pathway-related proteins were investigated by flow cytometry, TUNEL assay, western blot and immunofluorescence analysis respectively. Results In growth curve analysis, before KGN cells proliferated into confluence, UCMSCs-CM had no effect on cell proliferation. However, once the cells proliferate to contact each other, UCMSCs-CM significantly inhibited proliferation. Meanwhile, when KGN cells were implanted at high density, UCMSCs-CM could induce cell cycle arrest at G1 phase, inhibit cell migration, invasion and promote apoptosis. While it had no similar effect on KGN cells implanted at low density. In mechanism, the UCMSCs-CM treatment activated the Hippo pathway when KGN cells were implanted at high density. Consistently, the MST1/2 inhibitor, XMU-MP-1, inhibited the activation of the Hippo pathway induced by UCMSCs-CM treatment and accordingly declined the anti-tumor effect of UCMSCs-CM on KGN cells. Conclusions The effect of UCMSCs-CM on tumor cells is affected by cell density. UCMSCs-CM exerted anti-tumor effect on KGN cells by activating Hippo pathway to restore contact inhibition. Our results suggest that UCMSCs-CM is a promising therapeutic candidate for GCT treatment.

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Decidua Parietalis Mesenchymal Stem/Stromal Cells and Their Secretome Diminish the Oncogenic Properties of MDA231 Cells In Vitro

Cited by 9 publications

References 82 publications

BMSC–HNC Interaction: Exploring Effects on Bone Integrity and Head and Neck Cancer Progression

BMSC–HNC Interaction: Exploring Effects on Bone Integrity and Head and Neck Cancer Progression

Human umbilical cord mesenchymal stem cells conditioned medium exerts anti-tumor effects on KGN cells in a cell density-dependent manner through activation of the Hippo pathway

Human umbilical cord mesenchymal stem cells conditioned medium exerts anti-tumor effects on KGN cells in a cell density-dependent manner through activation of the Hippo pathway

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