Decipher the pharmacological mechanisms of raw and wine-processed Curculigo orchioides Gaertn. on bone destruction in rheumatoid arthritis rats using metabolomics

Liu, Xia; Huang, Mingchun; Wang, Lijuan; Yang, Czau‐Siung; Zhang, Mei; Wang, Qin

doi:10.1016/j.jep.2023.116395

Cited by 7 publications

(3 citation statements)

References 33 publications

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“…In order to gain a deeper comprehension of the pathological and physiological characteristics of DEPs, a Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was performed, resulting in the identification of several enriched pathways, including focal adhesion and salmonella infection (Figure B). Pentose phosphate pathway gained a high enrichment score, which is not only a pathway to balance energy and blood sugar but also partially involved in inhibiting bone destruction in rheumatoid arthritis rats . Several metabolic pathways of amino sugar and nucleotide sugar metabolism, cysteine and methionine metabolism, and glycolysis and gluconeogenesis were annotated, indicating the metabolic disturbance.…”

Section: Resultsmentioning

confidence: 99%

“…Pentose phosphate pathway gained a high enrichment score, which is not only a pathway to balance energy and blood sugar but also partially involved in inhibiting bone destruction in rheumatoid arthritis rats. 32 Several metabolic pathways of amino sugar and nucleotide sugar metabolism, cysteine and methionine metabolism, and glycolysis and gluconeogenesis were annotated, indicating the metabolic disturbance. Glycolysis is the pathway to burn glycose and produce ATP and pyruvate, while the aim of glucogenesis is synthesizing glucose.…”

Section: Quantification Of Identified Proteins Between Control and Hi...mentioning

confidence: 99%

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Proteomic Analysis of the Supernatant from Bone Marrow Mesenchymal Stem Cells under High Glucose Conditions

Li,

Wang,

et al. 2023

J. Proteome Res.

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Diabetes mellitus hinders the process of bone regeneration by inhibiting the function of mesenchymal stem cells (MSCs) through elevated glucose levels, thereby impeding osteointegration. The stem cell niche (SCN) plays a crucial role in determining the fate of stem cells by integrating various signals. However, the precise mechanism by which high glucose levels affect the SCN and subsequently influence the function of MSCs remains unclear. In this study, we employed proteomic analysis to identify proteins with altered expression in the extracellular matrix (ECM), aiming to elucidate the underlying mechanism. Three cell supernatants were collected from bone marrow mesenchymal stem cells (BMSCs) or BMSCs stimulated with high glucose (BMSCs +Hg). A total of 590 differentially expressed proteins were identified, which were found to be associated with the ECM, including aging, autophagy, and osteogenic differentiation. The findings of our study indicate that elevated glucose levels exert an influence on the molecular aspects of the SCN, potentially contributing to a better comprehension of the underlying mechanism.

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Section: Resultsmentioning

confidence: 99%

Section: Quantification Of Identified Proteins Between Control and Hi...mentioning

confidence: 99%

Proteomic Analysis of the Supernatant from Bone Marrow Mesenchymal Stem Cells under High Glucose Conditions

Li,

Wang,

et al. 2023

J. Proteome Res.

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“… 23 Both Epimedium breviconu Maxim (Epimedium) and Curculigo orchioides Gaertn (Curculigo orchioides), have the effects of nourishing kidney yang, strengthening bones and muscles and eliminating wind. 24 , 25 Pharmacological research has displayed that the flavonoids in Epimedium 9 , 26 or polysaccharides in Curculigo orchioides 27 , 28 could mediate osteoblasts' activities and then promote bone repair.…”

Section: Discussionmentioning

confidence: 99%

Epimedium‐Curculigo herb pair enhances bone repair with infected bone defects and regulates osteoblasts through LncRNA MALAT1/miR‐34a‐5p/SMAD2 axis

Miao,

Li,

Sheng

et al. 2024

J Cellular Molecular Medi

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Infected bone defects (IBDs) are the common condition in the clinical practice of orthopaedics. Although surgery and anti‐infective medicine are the firstly chosen treatments, in many cases, patients experience a prolonged bone union process after anti‐infective treatment. Epimedium‐Curculigo herb pair (ECP) has been proved to be effective for bone repair. However, the mechanisms of ECP in IBDs are insufficiency. In this study, Effect of ECP in IBDs was verified by micro‐CT and histological examination. Qualitative and quantitative analysis of the main components in ECP containing medicated serum (ECP‐CS) were performed. The network pharmacological approaches were then applied to predict potential pathways for ECP associated with bone repair. In addition, the mechanism of ECP regulating LncRNA MALAT1/miRNA‐34a‐5p/SMAD2 signalling axis was evaluated by molecular biology experiments. In vivo experiments indicated that ECP could significantly promote bone repair. The results of the chemical components analysis and the pathway identification revealed that TGF‐β signalling pathway was related to ECP. The results of in vitro experiments indicated that ECP‐CS could reverse the damage caused by LPS through inhibiting the expressions of LncRNA MALAT1 and SMAD2, and improving the expressions of miR‐34a‐5p, ALP, RUNX2 and Collagen type І in osteoblasts significantly. This research showed that ECP could regulate the TGF‐β/SMADs signalling pathway to promote bone repair. Meanwhile, ECP could alleviate LPS‐induced bone loss by modulating the signalling axis of LncRNA MALAT1/miRNA‐34a‐5p/ SMAD2 in IBDs.

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Orcinol glucoside targeted p38 as an agonist to promote osteogenesis and protect glucocorticoid-induced osteoporosis

He¹,

Zhao²,

Yao³

et al. 2023

Phytomedicine

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Decipher the pharmacological mechanisms of raw and wine-processed Curculigo orchioides Gaertn. on bone destruction in rheumatoid arthritis rats using metabolomics

Cited by 7 publications

References 33 publications

Proteomic Analysis of the Supernatant from Bone Marrow Mesenchymal Stem Cells under High Glucose Conditions

Proteomic Analysis of the Supernatant from Bone Marrow Mesenchymal Stem Cells under High Glucose Conditions

Epimedium‐Curculigo herb pair enhances bone repair with infected bone defects and regulates osteoblasts through LncRNA MALAT1/miR‐34a‐5p/SMAD2 axis

Orcinol glucoside targeted p38 as an agonist to promote osteogenesis and protect glucocorticoid-induced osteoporosis

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