Deciphering and simulating models of radiation genotoxicity with CRISPR/Cas9 systems

Vukmirovic, Dusan; Seymour, Colin; Mothersill, Carmel

doi:10.1016/j.mrrev.2020.108298

Cited by 1 publication

(1 citation statement)

References 89 publications

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“…Accordingly, EZH2, a novel biomarker, may serve as a novel target for treating TNBC. CRISPR/Cas9 system has been developed as a novel gene editing tool, capable of achieving high gene-targeted efficiency in different cell lines and small experimental animals, demonstrating that it is a promising gene therapy (18,19). To the best of our knowledge, however, the role of the CRISPR/Cas9 system in the editing of EZH2 in the treatment of BC remains unclear.…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9‑mediated EZH2 knockout suppresses the proliferation and migration of triple‑negative breast cancer cells

Mao

et al. 2023

Oncol Lett

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Triple-negative breast cancer (TNBC) is an aggressive subtype of BC characterized by extensive intratumoral heterogeneity. Compared with other types of BC, TNBC is more prone to invasion and metastasis. The aim of the present study was to determine whether adenovirus-mediated clustered regulatory interspaced short palindromic repeats (CRISPR)/Cas9 system is capable of effectively targeting enhancer of zeste homolog 2 (EZH2) in TNBC cells and lay an experimental basis for the investigation of the CRISPR/Cas9 system as a gene therapy for BC. In the present study, EZH2 was knocked out in MDA-MB-231 cells using the CRISPR/Cas9 gene editing tool to create EZH2-knockout (KO) group (EZH2-KO group). Moreover, the GFP knockout group (control group), and a blank group (Blank group), were employed. The success of vector construction and EZH2-KO were verified by T7 endonuclease I (T7EI) restriction enzyme digestion, mRNA detection and western blotting. Changes in proliferation and migration ability of MDA-MB-231 cells following gene editing were detected by MTT, wound healing, Transwell and in vivo tumor biology assays. As indicated by the results of mRNA and protein detection, the mRNA and protein expression of EZH2 were significantly downregulated in the EZH2-KO group. The difference in EZH2 mRNA and protein between the EZH2-KO and the two control groups was statistically significant. MTT, wound healing and transwell assay suggested that the proliferation and migration ability of MDA-MB-231 cells in the EZH2-KO group were significantly decreased after EZH2 knockout. In vivo, the tumor growth rate in the EZH2-KO group was significantly lower than that in the control groups. In brief, the present study revealed that the biological functions of tumor cells were inhibited after EZH2 knockout in MDA-MB-231 cells. The aforementioned findings suggested that EZH2 can have a key role in the development of TNBC.

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Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9‑mediated EZH2 knockout suppresses the proliferation and migration of triple‑negative breast cancer cells

Mao

et al. 2023

Oncol Lett

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Deciphering and simulating models of radiation genotoxicity with CRISPR/Cas9 systems

Cited by 1 publication

References 89 publications

CRISPR/Cas9‑mediated EZH2 knockout suppresses the proliferation and migration of triple‑negative breast cancer cells

CRISPR/Cas9‑mediated EZH2 knockout suppresses the proliferation and migration of triple‑negative breast cancer cells

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