Deciphering archaeal glycolipids of an extremely halophilic archaeon of the genus Halobellus by MALDI-TOF/MS

Lobasso, Simona; Pérez-Davó, Azahara; Vitale, Rita; Sánchez, Mercedes Monteoliva; Corcelli, Angela

doi:10.1016/j.chemphyslip.2014.11.002

Cited by 31 publications

(17 citation statements)

References 35 publications

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“…Two of these cardiolipins were identified as bisphosphatidylglycerols (BPGs) containing two AR cores (Figure 7A) and a BPG with an AR and an EXT-AR core (Figure 7B). These IPLs have been reported previously in other haloarchaea (Lattanzio et al, 2002; Corcelli, 2009; Angelini et al, 2012; Lobasso et al, 2015). Three novel tetraether cardiolipins ( IVa-c ) were found in three polysaccharide utilizing alkaliphilic haloarchaeal strains, “ Na.…”

Section: Resultssupporting

confidence: 86%

“…Archaea also produce bisphosphatidylglycerol diethers, analogous to the diacyl cardiolipins found in Bacteria and Eukarya (Corcelli et al, 2000; Lattanzio et al, 2002; Corcelli, 2009; Angelini et al, 2012; Yoshinaga et al, 2012). The membrane polar lipids of halophilic archaea, mostly from the class Halobacteria , have been characterized in a wide range of studies (Kushwaha et al, 1975; Kates et al, 1993; de Souza et al, 2009; Angelini et al, 2012; Dawson et al, 2012; Lobasso et al, 2015). Phosphatidylglycerophosphate methyl ester (PGP-Me) has often found to be the dominant polar headgroup.…”

Section: Introductionmentioning

confidence: 99%

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New Insights Into the Polar Lipid Composition of Extremely Halo(alkali)philic Euryarchaea From Hypersaline Lakes

et al. 2019

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We analyzed the polar membrane lipids of 13 strains of halo(alkali)philic euryarchaea from hypersaline lakes. Nine belong to the class Halobacteria, representing two functional groups: aerobic polysaccharide utilizers and sulfur-respiring anaerobes. The other four strains represent halo(alkali)philic methanogens from the class Methanomicrobia and a recently discovered class Methanonatronarchaeia. A wide range of polar lipids were detected across the 13 strains including dialkyl glycerol diethers (archaeols), membrane-spanning glycerol tetraethers and diether-based cardiolipins. The archaeols contained a range of core lipid structures, including combinations of C20 and C25 isoprenoidal alkyl chains, unsaturations, and hydroxy moieties. Several diether lipids were novel, including: (a) a phosphatidylglycerolhexose (PG-Gly) headgroup, (b) a N,N,N-trimethyl aminopentanetetrol (APT)-like lipid with a methoxy group in place of a hydroxy group on the pentanetetrol, (c) a series of polar lipids with a headgroup with elemental composition of either C12H25NO13S or C12H25NO16S2, and (d) novel cardiolipins containing a putative phosphatidylglycerolphosphate glycerophosphate (PGPGP) polar moiety. We found that the lipid distribution of the 13 strains could be generally separated into two groups, the methanogens (group) and the Halobacteria (class) based on the presence of specific core lipids. Within the methanogens, adaption to a high or more moderate salt concentration resulted in different ratios of glycerol dialkyl glycerol tetraethers (GDGTs) to archaeol. The methanogen Methanosalsum natronophilum AME2T had the most complex diether lipid composition of any of the 13 strains, including hydroxy archaeol and macrocyclic archaeol which we surmise is an order-specific membrane adaption. The zwitterionic headgroups APT and APT-Me were detected only in the Methanomicrobiales member Methanocalculus alkaliphilus AMF2T which also contained the highest level of unsaturated lipids. Only alkaliphilic members of the Natrialbales order contained PGPGP cardiolipins and the PG-Gly headgroup. The four analyzed neutrophilic members of the Halobacteria were characterized by the presence of sulfur-containing headgroups and glycolipids. The presence of cardiolipins with one or more i-C25 alkyl chains, generally termed extended archaeol (EXT-AR), in one of the Methanonatronarchaeia strains was unexpected as only one other order of methanogenic archaea has been reported to produce EXT-AR. We examined this further by looking into the genomic potential of various archaea to produce EXT-AR.

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Section: Resultssupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

New Insights Into the Polar Lipid Composition of Extremely Halo(alkali)philic Euryarchaea From Hypersaline Lakes

et al. 2019

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“…The profile of polar lipids of S2FP14 T was analysed by means of TLC in combination with MALDI-TOF mass spectrometry as recently described by Lobasso et al (2015). This profile was similar to those of the other species of the genus Halobellus .…”

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confidence: 77%

“…The structures of glycolipids, including a glycosyl-cardiolipin, were elucidated by post source decay (PSD) mass spectrometry analysis. Besides the monosulfated diglycosyl diphytanylglyceroldiether, two variants of a bis-sulfated diglycosyl diphytanylglyceroldiether were also identified; furthermore the glycosyl-cardiolipin was found to have the same structure as the analogue present in Halorubrum trapanicum and Haloferax volcanii (Lobasso et al , 2015).…”

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confidence: 99%

Halobellus ramosii sp. nov., an extremely halophilic archaeon isolated from a saline-wetland wildfowl reserve

Pérez-Davó

Aguilera

González-Paredes

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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An extremely halophilic archaeon, strain S2FP14 T , was isolated from a brine sample from the inland hypersaline lake Fuente de Piedra, a saline-wetland wildfowl reserve located in the province of Má laga in southern Spain. Colonies were red-pigmented and the cells were Gramstaining-negative, motile and pleomorphic. S2FP14T was able to grow in media containing 12.5-30 % (w/v) total salts (optimum 20 %) at pH 7-8.5 (optimum 7.5) and at 25-50 8C (optimum 37 8C

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“…TLC in combination with MALDI‐TOF/MS identified the structures of glycolipids including a glycosyl‐cardiolipin in two halophilic extremophilic microorganisms Halorubrum trapanicum and Haloferax volcanii isolated from the salt lake near Malaga (Spain) (Lobasso, Perez‐Davo, Vitale, Sanchez, & Corcelli, ). Besides the “normal” PtdGroP methyl ester, also phosphatidylglycerosulfate or bis‐PtdGro were identified as unusual sulfated bis‐diglycosyl diphytanylglyceroldiethers with molecular weights of over 1000 Da, and their presence was discussed in relation to high extracellular salinity.…”

Section: Introductionmentioning

confidence: 99%

Lipidomic Analysis: From Archaea to Mammals

Řezanka

Kolouchová

Gharwalová

et al. 2018

Lipids

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Lipids are among the most important organic compounds found in all living cells, from primitive archaebacteria to flowering plants or mammalian cells. They form part of cell walls and constitute cell storage material. Their biosynthesis and metabolism play key roles in faraway topics such as biofuel production (third-generation biofuels produced by microorganisms, e.g. algae) and human diseases such as adrenoleukodystrophy, Zellweger syndrome, or Refsum disease. Current lipidomic analysis requires fast and accurate processing of samples and especially their characterization. Because the number of possible lipids and, more specifically, molecular species of lipids is of the order of hundreds to thousands, it is necessary to process huge amounts of data in a short time. There are two basic approaches to lipidomic analysis: shotgun and liquid chromatography-mass spectometry. Both methods have their pros and cons. This review deals with lipidomics not according to the type of ionization or the lipid classes analyzed but according to the types of samples (organisms) under study. Thus, it is divided into lipidomic analysis of archaebacteria, bacteria, yeast, fungi, algae, plants, and animals.

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Deciphering archaeal glycolipids of an extremely halophilic archaeon of the genus Halobellus by MALDI-TOF/MS

Cited by 31 publications

References 35 publications

New Insights Into the Polar Lipid Composition of Extremely Halo(alkali)philic Euryarchaea From Hypersaline Lakes

New Insights Into the Polar Lipid Composition of Extremely Halo(alkali)philic Euryarchaea From Hypersaline Lakes

Halobellus ramosii sp. nov., an extremely halophilic archaeon isolated from a saline-wetland wildfowl reserve

Lipidomic Analysis: From Archaea to Mammals

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