“…Database Search, Label-Free Relative Quantification, Data Processing, and Identification of Cell Surface Proteins-The RAW files (Thermo Fisher Scientific) were further analyzed using the Progenesis LC-MS software (version 4.0, Nonlinear Dynamics, Newcastle, UK), as described previously (19), with the following changes: For peptide identification, using the search engine Mascot (Matrix Science, Release number 2.4), assuming tryptic digestion, one missed cleavage was allowed, a fragment ion mass tolerance of 0.6 Da and a parent ion tolerance of 10 ppm. Carbamidomethylation was set as fixed modification, methionine oxidation, and asparagine or glutamine deamidation were allowed as variable modifications.…”