Deciphering microbiota associated to Rhynchophorus ferrugineus in Italian samples: a preliminary study

Valzano, Matteo; Achille, Gabriele; Burzacca, Francesca; Damiani, Claudia; Scuppa, Patrizia; Ricci, Irene; Favia, Guido

doi:10.4081/jear.2012.e16

Cited by 12 publications

(11 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conversely, little attention was paid to the microbial community associated with RPW, although an intracellular primary endosymbiont has been described in weevils and classified as ‘ Candidatus Nardonella’ [ 29 – 32 ]. Regarding the gut bacterial community associated to RPW, only preliminary studies have been conducted [ 33 – 36 ], in some cases addressed to cultivable bacteria in order to identify potential insect pathogens useful in bio-control strategies [ 34 , 37 ]. Aerobic and facultative anaerobic bacteria ( Bacillus sp., Salmonella sp., Enterococcus sp.…”

Section: Introductionmentioning

confidence: 99%

Effects of the Diet on the Microbiota of the Red Palm Weevil (Coleoptera: Dryophthoridae)

Montagna

Chouaia

Mazza³

et al. 2015

PLoS ONE

View full text Add to dashboard Cite

Rhynchophorus ferrugineus, also known as the red palm weevil, is regarded as the major pest of palm trees. Although studies of the microbiota associated with this species have been performed in recent years, little attention has been dedicated to the influence of the diet in shaping the host bacterial community. Here, we investigated the influence of food sources (i.e. palm tissues vs apple based substrate) on the microbial diversity associated with RPW, which was compared with the microbiota associated with wild individuals of the sister species Rhynchophorus vulneratus. The bacterial characterization was performed using a culture independent approach, i.e. the 16S rRNA pyrotag, and a culture dependent approach for a subset of the samples, in order to obtain bacterial isolates from RPW tissues. The bacterial community appeared significantly influenced by diet. Proteobacteria resulted to be the most abundant clade and was present in all the specimens of the three examined weevil groups. Within Proteobacteria, Enterobacteriaceae were identified in all the organs analysed, including hemolymph and reproductive organs. The apple-fed RPWs and the wild R. vulneratus showed a second dominant taxon within Firmicutes that was scarcely present in the microbiota associated with palm-fed RPWs. A comparative analysis on the bacteria associated with the palm tissues highlighted that 12 bacterial genera out of the 13 identified in the plant tissues were also present in weevils, thus indicating that palm tissues may present a source for bacterial acquisition.

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of the Diet on the Microbiota of the Red Palm Weevil (Coleoptera: Dryophthoridae)

Montagna

Chouaia

Mazza³

et al. 2015

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Homogenates were then centrifuged at 10,000 ×g for 10 min at 4°C. The supernatant was collected in sterilized Eppendorf tubes and kept at −80°C until further use [21, 22]. …”

Section: Methodsmentioning

confidence: 99%

In Vitro Evaluation of Antimicrobial Activity of Alimentary Canal Extracts from the Red Palm Weevil,Rhynchophorus ferrugineusOlivier Larvae

Sewify

Hamada

Alhadrami

2017

BioMed Research International

View full text Add to dashboard Cite

The invasive red palm weevil, Rhynchophorus ferrugineus Olivier (Coleoptera: Curculionidae), is considered one of the world's most devastating insect pests to palm trees. It was observed that larvae of this pest are able to inhibit microbial growth on the rearing media when they start feeding and this observation has led us to study the effect of red palm weevils on various microbial species. The antimicrobial effect of extracts from different parts of the alimentary canal on Gram positive bacteria (Enterococcus faecalis and Staphylococcus aureus), Gram negative bacteria (Escherichia coli and Klebsiella spp.), Candida albicans, and Penicillium sp. was tested using the agar well diffusion method. All extracts inhibited the tested microbial species. Foregut extracts had the greatest zones of growth inhibition. Enterococcus faecalis, Staphylococcus aureus, and Penicillium sp. were significantly sensitive to the extracts and had the largest growth inhibition zones. It is concluded that the gut extracts contain potent antimicrobial activity and may provide a new source of antimicrobial peptides.

show abstract

“…Considering the huge economic and environmental damage caused by RPW, some attention has been paid to the efficacy of different chemical and biocontrol strategies [39] in regard to several bacterial species to use as biological control [10] [20].…”

Section: Identified Bacteria and Phylogenetic Relationmentioning

confidence: 99%

“…The RPW is one of the most destructive pests of date palms, the major fruit tree of arid, tropical and subtropical areas [8]. RPW is considered one of the serious pests for many palm species in the world, and withstand extreme weather [9] [10].…”

Section: Introductionmentioning

confidence: 99%

“…The identification of microbial community by using amplification of 16S rRNA: encoding DNA (rDNA) or 16S rRNA by PCR has revealed immense phylogenetic diversity [24] [25] [26]. The 16S rRNA molecules are of valued phylogenetic marker for microorganisms because of their universal distribution, constant function and sequence variation [10] [16]. Therefore, the goal of our study is to identify the culture-dependent bacterial community using PCR amplification of the 16S rRNA region and sequence analysis from chewed waste of RPW, collected from infested date palm trees.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification and Characterization of Bacterial Community Associated with the Chewed Feeding Waste of Red Palm Weevil in Infested Date Palm Trees

Mohamed¹,

Farooq²,

Pathak³

2020

ABB

View full text Add to dashboard Cite

Red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) (Coleoptera, Curculionidae), is considered one of the most damaging insect pests of date palms in the Kingdom of Bahrain. Large scale infestation of RPW to date palm trees leads to excessive feeding activity of the RPW larvae, which is carried out by microorganisms present within RPW and producing a wet fermenting material inside the trunk. Culture dependent-bacteria were isolated from feeding waste and identified by the sequencing of the 16S rRNA gene using 8F and 1492R universal primers. Among the culture-dependent isolated bacteria, 80% were identified by comparing 16S rRNA gene sequence in NCBI database, using BLAST program in GenBank. 85% of the identified bacteria were Gram-positive while the rest of them were Gram-negative. A high abundance of bacteria were from the Bacillaceae family and sixteen different species of Bacillus were identified in comparison with NCBI GenBank. The 16S rRNA gene sequences of identified bacterial strains have been submitted to GenBank. The phylogenetic relationship was studied using 16S rRNA gene sequences, the Gram-negative bacteria came in one clade while Gram-positive different Bacillus sp. and strains showed evolutionary closeness to each other and accordingly, they came in one major clade under three different sub-clades in the phylogenetic tree. The findings of new Bacillus strains in the natural habitat of the date plam trees in the Kingdom of Bahrain, pledge a vast area of research on RPW bio-control research arena.

show abstract

Deciphering microbiota associated to Rhynchophorus ferrugineus in Italian samples: a preliminary study

Cited by 12 publications

References 23 publications

Effects of the Diet on the Microbiota of the Red Palm Weevil (Coleoptera: Dryophthoridae)

Effects of the Diet on the Microbiota of the Red Palm Weevil (Coleoptera: Dryophthoridae)

In Vitro Evaluation of Antimicrobial Activity of Alimentary Canal Extracts from the Red Palm Weevil,Rhynchophorus ferrugineusOlivier Larvae

Identification and Characterization of Bacterial Community Associated with the Chewed Feeding Waste of Red Palm Weevil in Infested Date Palm Trees

Contact Info

Product

Resources

About