Deciphering of microbial community and antibiotic resistance genes in activated sludge reactors under high selective pressure of different antibiotics

Zhao, Renxin; Feng, Jie; Liu, Jie; Fu, Wenjie; Li, Xiaoyan; Li, Bing

doi:10.1016/j.watres.2018.12.034

Cited by 279 publications

(71 citation statements)

References 87 publications

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“…Recovery Rate (%) RSD (%, n = 5) Lake water 1 5 µmol L −1 4.870 µmol L −1 97.408 0.62% Tap water 1 10 µmol L −1 9.754 µmol L −1 97.544 0.94% Soil 1 12.240 µmol Kg −1 12.632 µmol Kg −1 103.206 0.82% Tap water 1 30 µmol L −1 27.517 µmol L −1 91.724 1.06% Lake water 1 40 µmol L −1 40.341 µmol L −1 100.853 0.62% Soil 1 30.600 µmol Kg −1 31.468 µmol Kg −1 102.837 0.88% Tap water 2 2 µmol L −1 1.844µmol L −1 92.203 0.84% Soil 2 9.180 µmol Kg −1 9.255 µmol Kg −1 100.826 0.81% Lake water 2 20 µmol L −1 19.399µmol L −1 96.996 1.05% Tap water 2 35 µmol L −1 34.514µmol L −1 98.613 0.94% Lake water 2 45 µmol L −1 45.311µmol L −1 100.691 0.88% Soil 2 30.600 µmol Kg −1 31.469 µmol Kg −1 102.844 5.35%…”

Section: Spiked Measured Concentrationmentioning

confidence: 99%

“…Antibiotics are natural, synthetic, or semisynthetic compounds that interfere with the development of other living cells [1]. The global consumption of antibiotics continues to increase, mainly due to the increase in the use of drugs and the demand for animal protein due to the increase in population [1,2]. Oxytetracycline (OTC) is a popular growth accelerator for livestock and aquaculture because of its low cost and strong antibacterial activity [3,4], while it can stay in edatope for more than one year, which is longer than other types of antibiotics [5].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Green Preparation of Fluorescent Nitrogen-Doped Carbon Quantum Dots for Sensitive Detection of Oxytetracycline in Environmental Samples

Gao

Wang

et al. 2020

Nanomaterials

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Nitrogen-doped carbon quantum dots (N-CQDs) with strong fluorescence were prepared by a one-step hydrothermal method using natural biomass waste. Two efficient fluorescent probes were constructed for selective and sensitive detection of oxytetracycline (OTC). The synthesized N-CQDs were characterized by UV-visible absorption spectra, fluorescence spectra, Fourier transform infrared spectroscopy (FT-IR), X-ray photon spectroscopy (XPS), atomic force microscopy (AFM), and high-resolution transmission electron microscopy (HRTEM), which proved that the synthesized N-CQDs surface were functionalized and had stable fluorescence performance. The basis of N-CQDs detection of OTC was discussed, and various reaction conditions were studied. Under optimized conditions, orange peel carbon quantum dots (ON-CQDs) and watermelon peel carbon quantum dots (WN-CQDs) have a good linear relationship with OTC concentrations in the range of 2–100 µmol L−1 and 0.25–100 µmol L−1, respectively. ON-CQDs and WN-CQDs were both successfully applied in detecting the OTC in pretreated tap water, lake water, and soil, with the recovery rate at 91.724–103.206%, and the relative standard deviation was less than 5.35%. The results showed that the proposed N-CQDs proved to be green and simple, greatly reducing the detection time for OTC in the determination environment.

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Section: Spiked Measured Concentrationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Green Preparation of Fluorescent Nitrogen-Doped Carbon Quantum Dots for Sensitive Detection of Oxytetracycline in Environmental Samples

Gao

Wang

et al. 2020

Nanomaterials

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“…The induction and development of ARGs could be related to the concentration effects of antibiotics on the bacterial groups harboring these genes [24]. For tracking the potential hosts of ARGs, the cooccurrence patterns between ARG subtypes and microbial taxa were explored by correlation analysis [25,26].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance Genes and Bacteria Detected in Hospital Sewage May Provide Valuable Information in Clinical Antimicrobial Resistance

Cai¹,

Sun²,

Yao³

et al. 2020

Preprint

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Aim: Clinical antimicrobial resistance (AMR) is a significant threat to public health, which is often unclear due to representative data from human populations that are challenging to obtain. Study the associations between consumption of antibiotics and antimicrobial resistance bacteria or genes (ARB or ARGs) that will benefit from elucidating the AMR. Methods: The details of antibiotics usage were calculated based on the actual consumption in the target hospital, ARB was detected by culture method, and ARGs were evaluated by metagenomics. Results: Our study revealed that culture-based single-indicator-strain approaches only capture the AMR in 16.17% infectious samples. 1573 bacterial species and 885 types of ARGs were found in the hospital sewage. The consumption of antibiotics influences the resistance profiles that were significant in E.coli, but the strength varies among bacteria. In all ARGs group, ARGs of aminoglycosides was the most common, followed by sulfonamide, tetracycline, phenicol, macrolides, and quinolones, comprising to 82.6% of all ARGs. Five hundred nineteen pairs of ARGs and bacterial species showed a significant correlation (r > 0.8). The co-occurrence patterns of bacteria- ARGs mirrors the AMR of the clinic. Antibiotic usage will affect the abundance of ARG in sewage, with a hysteresis effect. Conclusion: The ARGs- bacteria co-occurrence patterns from wastewater could be a valuable bio-indicator to reflect the emergence of ARB in the future. Developing a predictive risk model of AMR on this basis will facilitate the rational use of antibiotics.

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“…It has been widely acknowledged that antibiotic residue would promote the process of HGT, particularly for ARGs located in plasmids whose horizontal transfer mainly stems from the increased expression of conjugation machinery induced by antibiotics [ 48 ]. For example, cefotaxime treatment significantly increased the conjugation frequency of bla CTX−M−1 -bearing IncI1 resistance plasmid in a reference strain Escherichia coli MG1655 [ 49 ].…”

Section: Exogenous Compounds Promote Horizontal Genes Transfermentioning

confidence: 99%

Correlation between Exogenous Compounds and the Horizontal Transfer of Plasmid-Borne Antibiotic Resistance Genes

Tong

Shi

et al. 2020

Microorganisms

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The global spread of antibiotic resistance has posed a serious threat to public healthcare and undermined decades of progress made in the fight against bacterial infections. It has been demonstrated that the lack of novel effective antibiotics and rapid spread of antibiotic resistance genes via horizontal transfer in the ecosystem are mainly responsible for this crisis. Notably, plasmid-mediated horizontal transfer of antibiotic resistance genes (ARGs) is recognized as the most dominant dissemination pathway of ARGs in humans, animals and environmental settings. Antibiotic selective pressure has always been regarded as one of the crucial contributors to promoting the dissemination of antibiotic resistance through horizontal gene transfer (HGT). However, the roles of exogenous compounds and particularly non-antibiotic drugs in the spread of ARGs are still underappreciated. In this review, we first summarize the major pathways of HGT in bacteria, including conjugation, transformation, transduction and vesiduction. Subsequently, an overview of these compounds capable of promoting the HGT is presented, which guides to the formulation of more reasonable dosing regimens and drug residue standards in clinical practice. By contrast, these compounds that display an inhibition effect on HGT are also highlighted, which provides a unique strategy to minimize the spread of ARGs. Lastly, we discuss the implementations and challenges in bringing these HGT inhibitors into clinical trials.

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Deciphering of microbial community and antibiotic resistance genes in activated sludge reactors under high selective pressure of different antibiotics

Cited by 279 publications

References 87 publications

Green Preparation of Fluorescent Nitrogen-Doped Carbon Quantum Dots for Sensitive Detection of Oxytetracycline in Environmental Samples

Green Preparation of Fluorescent Nitrogen-Doped Carbon Quantum Dots for Sensitive Detection of Oxytetracycline in Environmental Samples

Antimicrobial Resistance Genes and Bacteria Detected in Hospital Sewage May Provide Valuable Information in Clinical Antimicrobial Resistance

Correlation between Exogenous Compounds and the Horizontal Transfer of Plasmid-Borne Antibiotic Resistance Genes

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