2022
DOI: 10.3389/fphar.2022.851554
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Deciphering the Effects and Mechanisms of Yi-Fei-San-Jie-pill on Non-Small Cell Lung Cancer With Integrating Network Target Analysis and Experimental Validation

Abstract: Non-small cell lung cancer (NSCLC), which accounts for 85% of lung cancer cases, calls for better therapy. Yi-Fei-San-Jie-pill (YFSJ), a well-applicated traditional Chinese medicine formula, was reported to be effective in the treatment of NSCLC. However, its anti-tumor mechanism still needs to be fully elucidated. Herein, a reliable preclinical orthotopic but not subcutaneous model of NSCLC in mice was established to evaluate the anti-cancer properties and further validate the mechanisms of YFSJ. A bioinforma… Show more

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Cited by 5 publications
(4 citation statements)
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“…Apoptosis-inducing proteins and organelle degradation induced cell death caused by living cell stress, which is the key link in the treatment of NSCLC. [27] Furthermore, flow cytometry results demonstrated that ORI increased the apoptosis rate of LLC cells ranging from 22.15% to 35.22% (Figure 1C). The extrinsic death receptor pathway and the intrinsic mitochondria pathway played an important role during the initiation and progression of apoptosis.…”
Section: Ori Leads To Cell Apoptosis Of Llcmentioning
confidence: 94%
“…Apoptosis-inducing proteins and organelle degradation induced cell death caused by living cell stress, which is the key link in the treatment of NSCLC. [27] Furthermore, flow cytometry results demonstrated that ORI increased the apoptosis rate of LLC cells ranging from 22.15% to 35.22% (Figure 1C). The extrinsic death receptor pathway and the intrinsic mitochondria pathway played an important role during the initiation and progression of apoptosis.…”
Section: Ori Leads To Cell Apoptosis Of Llcmentioning
confidence: 94%
“…Lewis lung carcinoma (LLC) cells obtained from Zhong Qiao Xin Zhou Biotechnology (LZQ0009; Shanghai, China) were incubated in DMEM medium (containing 10% fetal bovine serum and 1% penicillin) in 5% CO 2 at 37 ℃. To generate the NSCLC mouse model, LLC cells (80–90% growth) were subjected to trypsin digestion and were then collected and washed with phosphate-buffered saline (PBS), according to a previous study [ 23 ]. Cells were added to Matrigel at a 1:1 ratio after centrifugation, then 1 million cells were injected into each mouse.…”
Section: Nsclc Mouse Model Constructionmentioning
confidence: 99%
“…The LLC cells used for modeling were stably transfected with luciferase, which emitted fluorescence under substrate excitation. According to a previous study [ 23 ], IVIS lumina Series III (PerkinElmer, Waltham, MA, USA) was used to detect the inoculation results on the 5th day. Each mouse was given an intraperitoneal injection of 150 µL of D-luciferin (Luc-1G, 115144-35-9; Gold Bio-Technology, St. Louis, MO, USA) and observed for 15 min.…”
Section: Nsclc Mouse Model Constructionmentioning
confidence: 99%
“…Lung cancer has become the leading cause of death in human malignant tumors (Sung et al 2021). The incidence of non-small cell lung cancer (NSCLC) accounts for about 85% of the total incidence of lung cancer, because of its special biological behavior, it is prone to brain metastasis, the ve-year survival rate of NSCLC is only 15% in 2021 (Yang et al 2022;Tfayli et al 2021). Among which lung adenocarcinoma is the most common subtype (Thai et al 2021).…”
Section: Introductionmentioning
confidence: 99%