Deciphering the Genetics of Major End-Use Quality Traits in Wheat

Abstract

29Improving the end-use quality traits is one of the primary objectives in wheat breeding programs. 30 In the current study, a population of 127 recombinant inbred lines (RILs) derived from a cross 31 between Glenn (PI-639273) and Traverse (PI-642780) was developed and used to identify 32 quantitative trait loci (QTL) for 16 end-use quality traits in wheat. The phenotyping of these 16 33 traits was performed in nine environments in North Dakota, USA. The genotyping for the RIL 34 population was conducted using the wheat Illumina iSelect 90K SNP assay. A high-density 35 genetic linkage map consisting of 7,963 SNP markers identified a total of 76 additive QTL (A-36 QTL) and 73 digenic epistatic QTL (DE-QTL) associated with these traits. Overall, 12 stable 37 major A-QTL and three stable DE-QTL were identified for these traits, suggesting that both A-38 QTL and DE-QTL played an important role in controlling end-use quality traits in wheat. The 39 most significant A-QTL (AQ.MMLPT.ndsu.1B) was detected on chromosome 1B for mixograph 40 middle line peak time. The AQ.MMLPT.ndsu.1B A-QTL was located very close to the position 41 of the Glu-B1 gene encoding for a subunit of high molecular weight glutenin and explained up to 42 24.43% of phenotypic variation for mixograph MID line peak time. A total of 23 co-localized 43 QTL loci were detected, suggesting the possibility of the simultaneous improvement of the end-44 use quality traits through selection procedures in wheat breeding programs. Overall, the 45 information provided in this study could be used in marker-assisted selection to increase 46 selection efficiency and to improve the end-use quality in wheat. 47 48 49 50 51 4 Abbreviations 52 AACCI American Association of Cereal Chemists International 53 A-QTL additive QTL 54 BA baking absorption 55 BLV bread loaf volume 56 BLUP best linear unbiased predictor 57 BMT bake-mixing time 58 CBCL crumb color 59 CTCL crust color 60 cM centimorgans 61 DArT diversity arrays technology 62 DE-QTL digenic epistatic QTL 63 DO dough character 64 FE flour extraction 65 FHB Fusarium head blight 66 GPC grain protein content 67 HMW high molecular weight 68 HRSW hard red spring wheat 69 ICIM-ADD inclusive composite interval mapping with additive effects 70 ICIM-EPI inclusive composite interval mapping of epistatic QTL 71 LMW low molecular weight 72 MAS marker-assisted selection 73 5 MELS mixograph envelope left slope 74 MERS mixograph envelope right slope 75 MMLPI mixograph MID line peak integral 76 MMLPT mixograph MID line peak time 77 MMLPV mixograph MID line peak value 78 MMLPW mixograph MID line peak width 79 MMLTV mixograph MID line time * value 80 MIXOPA general mixograph pattern 81 NDSU North Dakota State University 82 NIR near-infrared reflectance 83 PPM parts per million 84 PV phenotypic variation 85 QTL quantitative trait loci 86 RCBD randomized complete block design 87 RFLP restriction fragment length polymorphisms 88 REML restricted maximum likelihood 89 SSD single seed descent 90 SKB Sandstedt, Kneen, and Blish 91 6 92W...

Genetic analysis of end‐use quality traits in wheat

“…(2012) and Naraghi et al. (2019) reported QTL for WA and BWA, respectively, on 6D. Based on their flanking markers, all these previously reported QTL either locate distantly from the QTL identified in the current study or do not have determined map positions.…”

“…However, not many QTL studies have been conducted for BMT, BWA, FAC, CS, MTOL, and LVReg. Quantitative trait loci for BMT were found on chromosomes 1A, 1B, 1D, 2A, 3A, 3B, 4B, 5D, 6D, 7A, 7B, and 7D (Maphosa et al., 2013; Naraghi et al., 2019; Simons et al., 2012; Tsilo, Hareland, Chao, & Anderson, 2011). Most recently, Naraghi et al.…”

“…Most recently, Naraghi et al. (2019) found QTL for BWA on 1B, 4A, 4B, 4D, and 6D. Quantitative trait loci for FAC was detected on 1A, 1B, 3B, 4A, 4B, 5A, 5B, 5D, and 7B (El‐Feki et al., 2013; Tsilo et al., 2011), while QTL for CS were reported on 5A and 6B (Groos et al., 2007).…”

“…Quantitative trait loci (QTL) have been found on all the 21 chromosomes for GPC and FY (Bordes et al., 2011; Fox et al., 2013; Goel et al., 2019; Kiszonas & Morris, 2018; Krystkowiak et al., 2017; Ma, Zhang, Yan, & Liu, 2012; Maphosa et al., 2013). Quantitative trait loci have also been detected on most chromosomes for flour protein content (FPC), MT, and WA in mixograph test, LV (Groos, Bervas, Chanliaud, & Charmet, 2007; Kiszonas & Morris, 2018; Naraghi et al., 2019; Nelson et al., 2006), and other grain quality traits including TW, kernel weight (KW), and kernel hardness (KH) (Boehm et al., 2017; Bordes et al., 2011; El‐Feki, Byrne, Reid, Lapitan, & Haley, 2013; Groos, Robert, Bervas, & Charmet, 2003; Huang et al., 2006; Krystkowiak et al., 2017; Kunert, Naz, Dedeck, Pillen, & Léon, 2007; J. Li et al., 2012; Y. Li et al., 2012, Li, Chao, Carver, & Wang, 2016; Li, Song, Zhou, Branlard, & Jia, 2009; Ma et al., 2012; Mohamed et al., 2013; Nelson et al., 2006; Sun, Marza, Ma, Carver, & Bai, 2010). The chromosomes that have no QTL reported so far include 1D, 3D, and 4A for FPC; 4B for MT; 3A, 5A, 6B, and 7AB for WA; 4A, 6A, and 7D for LV; 2B, 2D, 5D, and 6D for TW; 3D and 7B for KW; and 3D for KH.…”

Evaluating milling and baking quality associated with a Fusarium head blight resistance-enhancing genome deletion in wheat

“…Chromosome arm 3DL possesses 5318 genes (IWGSC 2018), and the terminal 20% of arm 3DL possesses approximately 1160 of these genes. Given the multitude of characteristics that collectively contribute to end use quality (Finney et al 1987) and their genetic complexity (e.g., Battenfield et al 2016;Jernigan et al 2018;Naraghi et al 2019), there seemed to be some likelihood that one or more of the genes from chromosome arm 3DL in A30 may impact one or more aspects of end use quality in A30.…”