Deciphering the messages carried by extracellular vesicles in hematological malignancies

Longjohn, Modeline N; Hudson, Jo-Anna; Smith, Nicole C.; Rise, Matthew L.; Moorehead, Paul; Christian, Sherri L.

doi:10.1016/j.blre.2020.100734

Cited by 19 publications

(17 citation statements)

References 132 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies have demonstrated the transfer and uptake of miRNA-containing EVs from multiple cells types, including immune cells [reviewed in ( 3 , 110 )]. EV transfer of miRNAs regulates gene expression in the target cell, thereby modulating its function ( 110 ).…”

Section: Discussionmentioning

confidence: 99%

“…Extracellular vesicles (EVs) are cell-derived, lipid bilayerenclosed particles that are secreted from many, if not all, cell types, including immune cells (1)(2)(3). Three categories of EVs have been described: exosomes (30-100 nm in diameter), which are formed when multivesicular bodies fuse with the plasma membrane to release intraluminal vesicles; microvesicles (100-1000 nm in diameter), which are formed from direct budding of the plasma membrane; and apoptotic bodies (>1 mM in diameter), which are formed from the blebbing membrane of an apoptotic cell (4,5).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells

et al. 2020

Self Cite

View full text Add to dashboard Cite

Cell-derived extracellular vesicles (EVs) participate in cell-cell communication via transfer of molecular cargo including genetic material like miRNAs. In mammals, it has previously been established that EV-mediated transfer of miRNAs can alter the development or function of immune cells, such as macrophages. Our previous research revealed that Atlantic salmon head kidney leukocytes (HKLs) change their morphology, phagocytic ability and miRNA profile from primarily “monocyte-like” at Day 1 to primarily “macrophage-like” at Day 5 of culture. Therefore, we aimed to characterize the miRNA cargo packaged in EVs released from these two cell populations. We successfully isolated EVs from Atlantic salmon HKL culture supernatants using the established Vn96 peptide-based pull-down. Isolation was validated using transmission electron microscopy, nanoparticle tracking analysis, and Western blotting. RNA-sequencing identified 19 differentially enriched (DE) miRNAs packaged in Day 1 versus Day 5 EVs. Several of the highly abundant miRNAs, including those that were DE (e.g. ssa-miR-146a, ssa-miR-155 and ssa-miR-731), were previously identified as DE in HKLs and are associated with macrophage differentiation and immune response in other species. Interestingly, the abundance relative of the miRNAs in EVs, including the most abundant miRNA (ssa-miR-125b), was different than the miRNA abundance in HKLs, indicating selective packaging of miRNAs in EVs. Further study of the miRNA cargo in EVs derived from fish immune cells will be an important next step in identifying EV biomarkers useful for evaluating immune cell function, fish health, or response to disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Regarding MRD monitoring in HMs, MFC and molecular analysis are the gold standard cell-based methods. The first technique, which can reach a sensitivity of 10 −3 to 10 −5 of leukemic blasts [35], is not recommended on PB due to the lower presence of leukemic cells [36]; instead, qRT-PCR, which has a sensitivity of 10 −4 to 10 −6 , relies on specific genetic or molecular targets and, thus, is applicable to fewer patients [35]. In this context, thanks to their abundance in biological fluids, specifically in blood, tumorderived EVs could overcome the deficiency of residual malignant cells making PB sampling suitable for MRD monitoring.…”

Section: Ev Clinical Applications In Hematological Malignanciesmentioning

confidence: 99%

“…Moreover, by homogenously circulating in various body fluids, EVs can capture the entire cancer heterogeneity; thus, they have a representative and global profile allowing to monitor tumor changes in real-time. It is also possible that EVs could be identified even when the presence of malignant cells is below the detection threshold of currently used techniques [36].…”

Section: Ev Clinical Applications In Hematological Malignanciesmentioning

confidence: 99%

Clinical relevance of extracellular vesicles in hematological neoplasms: from liquid biopsy to cell biopsy

et al. 2020

View full text Add to dashboard Cite

In the era of precision medicine, liquid biopsy is becoming increasingly important in oncology. It consists in the isolation and analysis of tumor-derived biomarkers, including extracellular vesicles (EVs), in body fluids. EVs are lipid bilayer-enclosed particles, heterogeneous in size and molecular composition, released from both normal and neoplastic cells. In tumor context, EVs are valuable carriers of cancer information; in fact, their amount, phenotype and molecular cargo, including proteins, lipids, metabolites and nucleic acids, mirror nature and origin of parental cells rendering EVs appealing candidates as novel biomarkers. Translation of these new potential diagnostic tools into clinical practice could deeply revolutionize the cancer field mainly for solid tumors but for hematological neoplasms, too.

show abstract

“…Exosomes, small (30-150 nm) bilayer membrane vesicles, are considered as cargo to transfer material (proteins, nucleic acids, lipids, metabolites, and organelles) from parental cells to targeted cells via ligand-receptor interactions. Exosomes with their biologic cargo were reported to play a pivotal role in mediating the immune response, tumor metastasis, angiogenesis, bone marrow reeducation, and drug resistance (Liu and Wang, 2019;Daassi et al, 2020;Longjohn et al, 2021). Accumulating evidence proves the potential of exosomes to serve as candidate biomarkers, therapeutic targets, drug delivery vehicles, or vaccines in a range of diseases (Sinha et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Circulating Exosomal MiR-107 Restrains Tumorigenesis in Diffuse Large B-Cell Lymphoma by Targeting 14-3-3η

Liu

Han

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Exosomes, nanometer-sized membranous vesicles in body fluids, have emerged as promising non-invasive biomarkers for cancer diagnosis. However, the function of exosomes in diffuse large B-cell lymphoma (DLBCL) remains elusive. This study aimed to investigate the role of exosomal miR-107 in lymphomagenesis and explore its clinical significance. In this study, decreased exosomal miR-107, miR-375-3p, and upregulated exosomal miR-485-3p were detected in the plasma of DLBCL patients and showed potential diagnostic value. Downregulated miR-107 expression was associated with advanced Ann Arbor stage, high IPI score, LDH, and β2-MG level in DLBCL patients. Overexpression of miR-107 by miR-107 Agomir significantly abrogated cell proliferation, induced apoptosis, and inhibited cell invasion in vitro, and repressed tumor growth in vivo. Moreover, the downregulation of miR-107 went in the opposite direction. The target genes of miR-107 were mainly enriched in the PI3K-Akt, Hippo, and AMPK signaling pathways. Notably, upregulated 14-3-3η (YWHAH) was suppressed by miR-107 in DLBCL, suggesting that miR-107 may restrain tumorigenesis by targeting 14-3-3η. In summary, this study unveils the function of miR-107 in lymphomagenesis, highlighting its potential as a diagnostic and prognostic indicator and as a new therapeutic target in the management of DLBCL.

show abstract

Deciphering the messages carried by extracellular vesicles in hematological malignancies

Cited by 19 publications

References 132 publications

Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells

Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells

Clinical relevance of extracellular vesicles in hematological neoplasms: from liquid biopsy to cell biopsy

Circulating Exosomal MiR-107 Restrains Tumorigenesis in Diffuse Large B-Cell Lymphoma by Targeting 14-3-3η

Contact Info

Product

Resources

About