Deciphering the microbial community structures and functions of wastewater treatment at high-altitude area

Abstract

Introduction: The proper operation of wastewater treatment plants is a key factor in maintaining a stable river and lake environment. Low purification efficiency in winter is a common problem in high-altitude wastewater treatment plants (WWTPs), and analysis of the microbial community involved in the sewage treatment process at high-altitude can provide valuable references for improving this problem.Methods: In this study, the bacterial communities of high- and low-altitude WWTPs were investigated using Illumina high-throughput sequencing (HTS). The interaction between microbial community and environmental variables were explored by co-occurrence correlation network.Results: At genus level, Thauera (5.2%), unclassified_Rhodocyclaceae (3.0%), Dokdonella (2.5%), and Ferribacterium (2.5%) were the dominant genera in high-altitude group. The abundance of nitrogen and phosphorus removal bacteria were higher in high-altitude group (10.2% and 1.3%, respectively) than in low-altitude group (5.4% and 0.6%, respectively). Redundancy analysis (RDA) and co-occurrence network analysis showed that altitude, ultraviolet index (UVI), pH, dissolved oxygen (DO) and total nitrogen (TN) were the dominated environmental factors (p < 0.05) affecting microbial community assembly, and these five variables explained 21.4%, 20.3%, 16.9%, 11.5%, and 8.2% of the bacterial assembly of AS communities.Discussion: The community diversity of high-altitude group was lower than that of low-altitude group, and WWTPs of high-altitude aeras had a unique microbial community structure. Low temperature and strong UVI are pivotal factors contributing to the reduced diversity of activated sludge microbial communities at high-altitudes.

Endogenous Melanin and Hydrogen‐Based Specific Activated Theranostics Nanoagents: A Novel Multi‐Treatment Paradigm for Rheumatoid Arthritis

“…[25] The high metabolic state of the RASFs and immune cells, along with inefficient vascular oxygen perfusion, lead to a highly hypoxic and weakly acidic microenvironment in the RA lesion. [26][27][28] Developing drugs for H 2 release under endogenous stimulus-responsiveness is one of effective strategies to achieve H 2 -controlled release. Hence, the hypoxic properties of the RA microenvironment have been designed to construct hypoxia-responsive nanoagents that precisely release functionalizing components, enabling dual-modal imaging-guided multi-treatment (PTT, antioxidative, and anti-inflammatory).…”

The Influence of Atmospheric Pressure and Organic Loading on the Sustainability of Simultaneous Nitrification and Denitrification

“…However, the decreased air pressure in plateau regions obstructs the efficient transfer of oxygen from air to water, resulting in notably low levels of saturated dissolved oxygen. Moreover, the reduced oxygen levels in the high-altitude atmosphere escalate the energy consumption in traditional biological nitrogen removal processes, presenting substantial challenges in mitigating these environmental concerns effectively [1][2][3]. Simultaneous nitrification and denitrification (SND), known for its low oxygen demand and rapid reaction rate [4,5], stands out as a promising and environmentally sustainable solution for municipal wastewater treatment in high-altitude regions [3].…”