Deciphering the miRNA transcriptome of Rongchang pig longissimus dorsi at weaning and slaughter time points

Chen, Xiaochang; Zhao, Chen; Dou, Mingle; Sun, Yunmei; Yu, Taiyong; Pang, Wei; Yang, Gongshe

doi:10.1111/jpn.13314

Cited by 11 publications

(9 citation statements)

References 37 publications

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“…Overexpression of miR-22-3p promotes myoblast differentiation and reduces proliferation in mouse myoblasts ( Marzi et al, 2012 ). Similar results were seen in porcine satellite cells ( Chen et al, 2020 ). miR-127 plus others have been selected for further testing as important miRNA and their potential role in muscle development and meat quality ( Iqbal et al, 2020 ).…”

Section: Discussionsupporting

confidence: 85%

“…miR-29 was found in muscle and satellite cell cultures, however satellite cell cultures had higher abundance of the miRNA. Postnatally miR-29a was found to be upregulated and miR-127 was down regulated from weaning to maturity in pigs ( Chen et al, 2020 ). A similar trend with miR-29a was found in the current study, however miR-127 was only down regulated during the prenatal to early postnatal comparisons (PN2 vs. PN3, PN3 vs. PW1, and PW1 vs. PW2).…”

Section: Discussionmentioning

confidence: 99%

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miRNA transcriptome and myofiber characteristics of lamb skeletal muscle during hypertrophic growth1

et al. 2022

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Postnatal muscle growth is achieved through hypertrophy of the muscle fibers and is impacted by the activity of satellite cells, the quiescent muscle stem cell. Several miRNAs are preferentially expressed in skeletal muscle and could provide a mechanism for increasing muscle hypertrophy through satellite cell proliferation and/or differentiation. The objectives of this study were to: 1) Characterize the miRNA transcriptome of the longissimus thoracis et lumborum muscle at several developmental timepoints [gestational d 85 (PN1), 110 (PN2), 133 (PN3), postnatal d 42 (PW1), 65 (PW2), 243 (MAT)] during muscle hypertrophy in lambs, and 2) examine miR-29a, identified in sequencing to be differentially regulated across development, loss of function on satellite cell proliferation and differentiation. Muscle fiber characteristics showed drastic increases (p < 0.0001) in fiber size and alterations in muscle fiber type occur during pre and postnatal development. miRNA sequencing comparisons were performed in developmental order (PN1 vs. PN2, PN2 vs. PN3, PN3 vs. PW1, PW1 vs. PW2, PW2 vs. MAT). There were 184 differentially expressed (Padj < 0.05) miRNA, 142 unique miRNA, from all 5 comparisons made. The transitional stage (PN3 vs. PW1) had the largest number (115) of differentially expressed miRNA. Inhibition of miR-29a in satellite cell culture increased (p < 0.05) cell proliferation and differentiation capacity. Characterization of the miRNA transcriptome provides valuable insights into the miRNA involved in muscle fiber hypertrophy and the potential importance of the transitional period.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

miRNA transcriptome and myofiber characteristics of lamb skeletal muscle during hypertrophic growth1

et al. 2022

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“…Four down-regulated known caprine miRNAs (miR-127-3p, miR-217-5p, miR-410-3p, and miR-487b-3p) in LC goats attracted our attention. The miR-127-3p has been reported to inhibit proliferation of C2C12 myoblast ( 50 ), as well as proliferation and differentiation of porcine skeletal muscle satellite cells ( 51 ). Similarly, miR-217-5p and miR-487b-3p inhibited the differentiation of skeletal muscle cells in rats ( 52 ) and mice ( 53 ), respectively.…”

Section: Discussionmentioning

confidence: 99%

Deep Small RNA Sequencing Reveals Important miRNAs Related to Muscle Development and Intramuscular Fat Deposition in Longissimus dorsi Muscle From Different Goat Breeds

et al. 2022

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MicroRNAs (miRNAs) are a class of small non-coding RNAs that have been shown to play important post-transcriptional regulatory roles in the growth and development of skeletal muscle tissues. However, limited research into the effect of miRNAs on muscle development in goats has been reported. In this study, Liaoning cashmere (LC) goats and Ziwuling black (ZB) goats with significant phenotype difference in meat production performance were selected and the difference in Longissimus dorsi muscle tissue expression profile of miRNAs between the two goat breeds was then compared using small RNA sequencing. A total of 1,623 miRNAs were identified in Longissimus dorsi muscle tissues of the two goat breeds, including 410 known caprine miRNAs, 928 known species-conserved miRNAs and 285 novel miRNAs. Of these, 1,142 were co-expressed in both breeds, while 230 and 251 miRNAs were only expressed in LC and ZB goats, respectively. Compared with ZB goats, 24 up-regulated miRNAs and 135 miRNAs down-regulated were screened in LC goats. A miRNA-mRNA interaction network showed that the differentially expressed miRNAs would target important functional genes associated with muscle development and intramuscular fat deposition. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that the target genes of differentially expressed miRNAs were significantly enriched in Ras, Rap 1, FoxO, and Hippo signaling pathways. This study suggested that these differentially expressed miRNAs may be responsible for the phenotype differences in meat production performance between the two goat breeds, thereby providing an improved understanding of the roles of miRNAs in muscle tissue of goats.

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“…These miRNAs which playing significant roles in skeletal muscle development could be used as biomarkers after further validation. Further, the authors also reported that miR-217 could act as an inhibitor of porcine satellite cells (PSCs) proliferation and differentiation through downregulating the expression of cyclin B, cyclin D, as well as proliferating cell nuclear antigen (PCNA) and MyoG and MyHC respec-tively [61]. Remarkably, miR-208b and miR-499 are responsible for the transcriptional repression of the slow-twitch protein gene SRY-box transcription factor 6 (Sox6).…”

Section: Role Of Mirnas In Myogenesismentioning

confidence: 99%

“…Other miRNAs involved in porcine skeletal muscle development include miRNA-21, a novel myogenic miRNA that regulates skeletal muscle development through controlling the Pphosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) signaling by targeting the transforming growth factor-β I (TGFβI) gene [18]. In PSCs in skeletal muscle miR-22 inhibits the proliferation through targeting the cyclin D1, cyclin B1, and p21 but promotes the differentiation through regulating the expression of MyoG and MyHC [61]. Further, miR-27a inhibits the expression of MyoG and it also regulates the protein kinase B/forkhead box protein O1 (Akt/FoxO1) signal pathway during porcine myogenesis [75] while miR27b acts to promote PSCs myogenesis by targeting MyoD family inhibitor (MDFI) [76].…”

Section: Role Of Mirnas In Myogenesismentioning

confidence: 99%

Role of microRNAs in myogenesis and their effects on meat quality in pig — A review

Iqbal

Ping

Ali

et al. 2020

Asian-Australas J Anim Sci

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The demand for food is increasing day by day because of the increasing global population. Therefore, meat, the easiest and largely available source of protein, needs to be produced in large amounts with good quality. The pork industry is a significant shareholder in fulfilling the global meat demands. Notably, myogenesis- development of muscles during embryogenesis- is a complex mechanism which culminates in meat production. But the molecular mechanisms which govern the myogenesis are less known. The involvement of miRNAs in myogenesis and meat quality, which depends on factors such as myofiber composition and intramuscular fat contents which determine the meat color, flavor, juiciness, and water holding capacity, are being extrapolated to increase both the quantity and quality of pork. Various kinds of microRNAs (miRNAs), miR-1, miR-21, miR22, miR-27, miR-34, miR-127, miR-133, miR-143, miR-155, miR-199, miR-206, miR-208, miR-378, and miR-432 play important roles in pig skeletal muscle development. Further, the quality of meat also depends upon myofiber which is developed through the expression of different kinds of miRNAs at different stages. This review will focus on the mechanism of myogenesis, the role of miRNAs in myogenesis, and meat quality with a focus on the pig.

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Deciphering the miRNA transcriptome of Rongchang pig longissimus dorsi at weaning and slaughter time points

Cited by 11 publications

References 37 publications

miRNA transcriptome and myofiber characteristics of lamb skeletal muscle during hypertrophic growth1

miRNA transcriptome and myofiber characteristics of lamb skeletal muscle during hypertrophic growth1

Deep Small RNA Sequencing Reveals Important miRNAs Related to Muscle Development and Intramuscular Fat Deposition in Longissimus dorsi Muscle From Different Goat Breeds

Role of microRNAs in myogenesis and their effects on meat quality in pig — A review

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