Deciphering the Role of a SLOG Superfamily Protein YpsA in Gram-Positive Bacteria

Brzozowski, R; Huber, Mirella; Burroughs, A. Maxwell; Graham, Gianni; Walker, Merryck; Alva, Sameeksha S; Aravind, L.; Eswara, Prahathees J.

doi:10.3389/fmicb.2019.00623

Cited by 17 publications

(26 citation statements)

References 60 publications

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“…4C to H), as discussed previously (Fig. 1A) (7). The quantification of cell lengths is shown in Fig.…”

Section: Resultssupporting

confidence: 54%

“…The structure of YpsA and another SLOG superfamily member DprA, a single-stranded DNA binding protein, is similar. Thus, it is possible YpsA also binds DNA or nucleotides such as NAD or ADP-ribose, as speculated previously (7). We undertook this study to shed light on the possible pathways through which YpsA functions.…”

Section: Discussionmentioning

confidence: 80%

“…It is important to note that E55D mutation allows YpsA-GFP focus formation ( Fig. 2J), while the negative charge reversal in an E55Q mutation did not (7). Both E55D and E55Q mutants are unable to elicit filamentation upon overproduction.…”

Section: Discussionmentioning

confidence: 93%

“…Although many factors involved in facilitating the cell division process have been discovered in B. subtilis (3) and E. coli (2), our understanding is still incomplete even in these model organisms since evidence of yet-to-be-uncovered factors exists (5,6). We reported previously that YpsA is such a factor, which appears to play a role in cell division in B. subtilis and S. aureus (7). The precise mechanism by which YpsA functions remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…YpsA is conserved in the Firmicutes phylum of Gram-positive bacteria and appears to be in a syntenous relationship with a known cell division protein, GpsB (7). The crystal structure of B. subtilis YpsA was solved by a structural genomics group in 2006 (PDB ID 2NX2) (8).…”

mentioning

confidence: 99%

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Interdependent YpsA- and YfhS-Mediated Cell Division and Cell Size Phenotypes in Bacillus subtilis

Brzozowski

Tomlinson

Sacco

et al. 2020

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Although many bacterial cell division factors have been uncovered over the years, evidence from recent studies points to the existence of yet-to-be-discovered factors involved in cell division regulation. Thus, it is important to identify factors and conditions that regulate cell division to obtain a better understanding of this fundamental biological process. We recently reported that in the Gram-positive organisms Bacillus subtilis and Staphylococcus aureus, increased production of YpsA resulted in cell division inhibition. In this study, we isolated spontaneous suppressor mutations to uncover critical residues of YpsA and the pathways through which YpsA may exert its function. Using this technique, we were able to isolate four unique intragenic suppressor mutations in ypsA (E55D, P79L, R111P, and G132E) that rendered the mutated YpsA nontoxic upon overproduction. We also isolated an extragenic suppressor mutation in yfhS, a gene that encodes a protein of unknown function. Subsequent analysis confirmed that cells lacking yfhS were unable to undergo filamentation in response to YpsA overproduction. We also serendipitously discovered that YfhS may play a role in cell size regulation. Finally, we provide evidence showing a mechanistic link between YpsA and YfhS. IMPORTANCE Bacillus subtilis is a rod-shaped Gram-positive model organism. The factors fundamental to the maintenance of cell shape and cell division are of major interest. We show that increased expression of ypsA results in cell division inhibition and impairment of colony formation on solid medium. Colonies that do arise possess compensatory suppressor mutations. We have isolated multiple intragenic (within ypsA) mutants and an extragenic suppressor mutant. Further analysis of the extragenic suppressor mutation led to a protein of unknown function, YfhS, which appears to play a role in regulating cell size. In addition to confirming that the cell division phenotype associated with YpsA is disrupted in a yfhS-null strain, we also discovered that the cell size phenotype of the yfhS knockout mutant is abolished in a strain that also lacks ypsA. This highlights a potential mechanistic link between these two proteins; however, the underlying molecular mechanism remains to be elucidated.

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“…4C to H), as discussed previously (Fig. 1A) (7). The quantification of cell lengths is shown in Fig.…”

Section: Resultssupporting

confidence: 54%

Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Interdependent YpsA- and YfhS-Mediated Cell Division and Cell Size Phenotypes in Bacillus subtilis

Brzozowski

Tomlinson

Sacco

et al. 2020

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Green Biosynthesis of Silver Nanoparticles Utilizing Monstera deliciosa Leaf Extract and Estimation of its Antimicrobial Characteristics

Shirsul,

Tripathi,

Ankamwar

2024

Part & Part Syst Charact

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Herein, single step biosynthesis of silver nanoparticles (AgNPs) is reported by using AgNO3 and Monstera deliciosa (MD) leaf extract. Spherical AgNPs are obtained with difference in particle sizes and antibacterial activities using two sets of MD leaf extract having different heating periods 5 and 10 min. They are characterized by UV–vis spectroscopy, X‐ray diffraction (XRD), Zeta potential, Attenuated Total Reflectance Infrared Spectroscopy (ATR‐IR), and Field Emission Scanning Electron Microscopy (FE‐SEM). Dynamic Light Scattering (DLS) studies propose two types of AgNPs have hydrodynamic diameters of 104 and 80 nm. Nuclear Magnetic Resonance (NMR), Gas Chromatography‐Mass Spectrometry (GC‐MS), and High Resolution−Mass Spectrometry (HR‐MS) studies, which reveal the presence of various phytochemicals found in MD leaf extract. These biomolecules play a vital role as stabilizing and reducing agents to fabricate stable AgNPs. These AgNPs exhibit strong antibacterial activities having effective zone of inhibition for both Gram‐positive (Staphylococcus aureus, Bacillus subtilis) and Gram‐negative (Escherichia coli, Pseudomonas aeruginosa) microorganisms, nonetheless AgNPs against different fungi (Penicillium sp., Aspergillus flavus, Fusarium oxysporum, Rhizoctonia solani) show non‐antifungal characteristics because of their inert nature. This study suggests that AgNPs can be used in treating bacterial infections and drug delivery also for several therapeutic and diagnostic applications.

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Multi-omics reveal an overlooked pathway for H2S production induced by bacterial biogenesis from composting

Yang,

Sun,

Zhang

et al. 2025

Journal of Hazardous Materials

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Deciphering the Role of a SLOG Superfamily Protein YpsA in Gram-Positive Bacteria

Cited by 17 publications

References 60 publications

Interdependent YpsA- and YfhS-Mediated Cell Division and Cell Size Phenotypes in Bacillus subtilis

Interdependent YpsA- and YfhS-Mediated Cell Division and Cell Size Phenotypes in Bacillus subtilis

Green Biosynthesis of Silver Nanoparticles Utilizing Monstera deliciosa Leaf Extract and Estimation of its Antimicrobial Characteristics

Multi-omics reveal an overlooked pathway for H2S production induced by bacterial biogenesis from composting

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