2020
DOI: 10.1128/mbio.02273-19
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Deciphering the Rules Underlying Xenogeneic Silencing and Counter-Silencing of Lsr2-like Proteins Using CgpS of Corynebacterium glutamicum as a Model

Abstract: Lsr2-like nucleoid-associated proteins play an important role as xenogeneic silencers (XS) of horizontally acquired genomic regions in actinobacteria. In this study, we systematically analyzed the in vivo constraints underlying silencing and counter-silencing of the Lsr2-like protein CgpS in Corynebacterium glutamicum. Genome-wide analysis revealed binding of CgpS to regions featuring a distinct drop in GC profile close to the transcription start site (TSS) but also identified an overrepresented motif with mul… Show more

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Cited by 18 publications
(53 citation statements)
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“…The Lsr2 protein plays both architectural and regulatory roles and is highly conserved throughout the Actinobacteria, including Corynebacterium 20 , 21 , the pathogenic Mycobacterium, and the antibiotic-producing Streptomyces 22 . Notably, Lsr2 is considered to be essential in M. tuberculosis .…”
Section: Discussionmentioning
confidence: 99%
“…The Lsr2 protein plays both architectural and regulatory roles and is highly conserved throughout the Actinobacteria, including Corynebacterium 20 , 21 , the pathogenic Mycobacterium, and the antibiotic-producing Streptomyces 22 . Notably, Lsr2 is considered to be essential in M. tuberculosis .…”
Section: Discussionmentioning
confidence: 99%
“…An explanation for this observation probably lies in the binding properties of CgpS. As shown by Wiechert and colleagues, binding of CgpS requires high AT content as well as a specific binding motif [ 57 ]. CL31, in contrast, shows a similar GC content as the host (54%) and thus might be able to escape the silencing by CgpS.…”
Section: Discussionmentioning
confidence: 99%
“…An explanation for this observation probably lies in the binding properties of CgpS. As shown by Wiechert and colleagues, binding of CgpS requires high AT content as well as a specific binding motif [59]. CL31, in contrast, shows a similar GC content as the host (54 %) and thus might be able to escape the silencing by CgpS.…”
Section: Discussionmentioning
confidence: 99%