Deciphering the Symbiotic Significance of Quorum Sensing Systems of Sinorhizobium fredii HH103

Acosta‐Jurado, Sebastián; Alias‐Villegas, Cynthia; Almozara, Andrés; Espuny, M. Rosario; Vinardell, José‐María; Pérez‐Montaño, Francisco

doi:10.3390/microorganisms8010068

Cited by 10 publications

(10 citation statements)

References 73 publications

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“…In contrast to that found in R. leguminosarum and S. meliloti, S. fredii HH03 EPS production is not influenced by QS mechanisms since this strain lacks a functional copy of the expR gene [157]. In contrast, the two QS systems identified in strain NGR234 have a positive effect on EPS production, meanwhile they repress genes involved in motility and chemotaxis [158].…”

Section: Sinorhizobium Frediimentioning

confidence: 72%

Rhizobial Exopolysaccharides: Genetic Regulation of Their Synthesis and Relevance in Symbiosis With Legumes

Acosta‐Jurado¹,

Fuentes-Romero²,

Ruíz-Sainz³

et al. 2021

Preprint

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Rhizobia are soil proteobacteria able to engage in a nitrogen-fixing symbiotic interaction with legumes which involves root rhizobial infection and bacterial invasion of new organs formed by the plant in response to the presence of appropriate bacterial partners. This interaction relies on a complex molecular dialogue between both symbionts. Bacterial N-acetyl-glucosamine oligomers called Nod factors are indispensable in most cases for early steps of the symbiotic interaction. In addition, different rhizobial surface polysaccharides, such as exopolysaccharides (EPS), may also be symbiotically relevant. EPS are acidic polysaccharides located out of the cell with little or no cell association that carry out important roles both in free-life and in symbiosis. EPS production is very complexly modulated and, frequently, co-regulated with that of Nod factors, but the type of co-regulation varies depending on the rhizobial strain. Many studies point out a signalling role of EPS-derived oligosaccharides in root infection and nodule invasion but, in certain symbiotic couples, EPS can be dispensable for a successful interaction. In summary, the complex regulation of the production of rhizobial EPS varies in different rhizobia and the relevance of this polysaccharide in symbiosis with legumes depends on the specific interacting couple.

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Section: Sinorhizobium Frediimentioning

confidence: 72%

Rhizobial Exopolysaccharides: Genetic Regulation of Their Synthesis and Relevance in Symbiosis With Legumes

Acosta‐Jurado¹,

Fuentes-Romero²,

Ruíz-Sainz³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…In contrast to that found in R. leguminosarum and S. meliloti, S. fredii HH103 EPS production is not influenced by QS mechanisms since this strain lacks a functional copy of the expR gene [165]. However, the two QS systems identified in strain NGR234 have a positive effect on EPS production and repress genes involved in motility and chemotaxis [166].…”

Section: Sinorhizobium Frediimentioning

confidence: 82%

Rhizobial Exopolysaccharides: Genetic Regulation of Their Synthesis and Relevance in Symbiosis with Legumes

Acosta‐Jurado

Fuentes-Romero

Ruíz-Sainz

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Rhizobia are soil proteobacteria able to engage in a nitrogen-fixing symbiotic interaction with legumes that involves the rhizobial infection of roots and the bacterial invasion of new organs formed by the plant in response to the presence of appropriate bacterial partners. This interaction relies on a complex molecular dialogue between both symbionts. Bacterial N-acetyl-glucosamine oligomers called Nod factors are indispensable in most cases for early steps of the symbiotic interaction. In addition, different rhizobial surface polysaccharides, such as exopolysaccharides (EPS), may also be symbiotically relevant. EPS are acidic polysaccharides located out of the cell with little or no cell association that carry out important roles both in free-life and in symbiosis. EPS production is very complexly modulated and, frequently, co-regulated with Nod factors, but the type of co-regulation varies depending on the rhizobial strain. Many studies point out a signalling role for EPS-derived oligosaccharides in root infection and nodule invasion but, in certain symbiotic couples, EPS can be dispensable for a successful interaction. In summary, the complex regulation of the production of rhizobial EPS varies in different rhizobia, and the relevance of this polysaccharide in symbiosis with legumes depends on the specific interacting couple.

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“…In S. fredii , the traI and sinI genes are essential for the biosynthesis of short- and long-chain AHLs, respectively [ 98 ]. Indeed, no 3-OXO-C8-HSL was detected from the traI and traI exoF mutants of SF2 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Rhizobial migration toward roots mediated by FadL-ExoFQP modulation of extracellular long-chain AHLs

Zhang

et al. 2023

The ISME Journal

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Migration from rhizosphere to rhizoplane is a key selecting process in root microbiome assembly, but not fully understood. Rhizobiales members are overrepresented in the core root microbiome of terrestrial plants, and here we report a genome-wide transposon-sequencing of rhizoplane fitness genes of beneficial Sinorhizobium fredii on wild soybean, cultivated soybean, rice, and maize. There were few genes involved in broad-host-range rhizoplane colonization. The fadL mutant lacking a fatty acid transporter exhibited high colonization rates, while mutations in exoFQP (encoding membrane proteins directing exopolysaccharide polymerization and secretion), but not those in exo genes essential for exopolysaccharide biosynthesis, led to severely impaired colonization rates. This variation was not explainable by their rhizosphere and rhizoplane survivability, and associated biofilm and exopolysaccharide production, but consistent with their migration ability toward rhizoplane, and associated surface motility and the mixture of quorum-sensing AHLs (N-acylated-L-homoserine lactones). Genetics and physiology evidences suggested that FadL mediated long-chain AHL uptake while ExoF mediated the secretion of short-chain AHLs which negatively affected long-chain AHL biosynthesis. The fadL and exoF mutants had elevated and depleted extracellular long-chain AHLs, respectively. A synthetic mixture of long-chain AHLs mimicking that of the fadL mutant can improve rhizobial surface motility. When this AHL mixture was spotted into rhizosphere, the migration toward roots and rhizoplane colonization of S. fredii were enhanced in a diffusible way. This work adds novel parts managing extracellular AHLs, which modulate bacterial migration toward rhizoplane. The FadL-ExoFQP system is conserved in Alphaproteobacteria and may shape the “home life” of diverse keystone rhizobacteria.

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Deciphering the Symbiotic Significance of Quorum Sensing Systems of Sinorhizobium fredii HH103

Cited by 10 publications

References 73 publications

Rhizobial Exopolysaccharides: Genetic Regulation of Their Synthesis and Relevance in Symbiosis With Legumes

Rhizobial Exopolysaccharides: Genetic Regulation of Their Synthesis and Relevance in Symbiosis With Legumes

Rhizobial Exopolysaccharides: Genetic Regulation of Their Synthesis and Relevance in Symbiosis with Legumes

Rhizobial migration toward roots mediated by FadL-ExoFQP modulation of extracellular long-chain AHLs

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