Decline of <i>Paulownia tomentosa</i> caused by <i>Trametes hirsuta</i> in Serbia

Milenković, Ivan; Tomšovský, Michal; Karadžić, D.; Veselinović, Milorad

doi:10.1111/efp.12438

Cited by 9 publications

(5 citation statements)

References 3 publications

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“…This indicated the potentially strong ligninolytic activity of strain X-13. The morphological analysis of strain showed that the colony of strain X-13 was milky white with the dense hyphae, and it revealed a thread-like septate mycelium without any reproductive structures (Additional file 2 : Figure S2), which were similar to those of reported Trametes hirsuta genus strains [ 23 , 24 ]. Moreover, the ITS region sequence of strain X-13 (GenBank: MT995079) showed 99% similarity with that of white-rot basidiomycete Trametes hirsuta JL-22-2 according to NCBI BLAST algorithm analysis.…”

Section: Resultssupporting

confidence: 68%

Elucidation of ligninolysis mechanism of a newly isolated white-rot basidiomycete Trametes hirsuta X-13

et al. 2021

Biotechnol Biofuels

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Background Lignin is a complex aromatic heteropolymer comprising 15–30% dry weight of the lignocellulose. The complex structural characteristic of lignin renders it difficult for value-added utilization. Exploring efficient lignin-degrading microorganisms and investigating their lignin-degradation mechanisms would be beneficial for promoting lignin valorization. In this study, a newly isolated white-rot basidiomycete, Trametes hirsuta X-13, with capacity to utilize alkaline lignin as the sole substrate was investigated. Results The analysis of the fermentation properties of T. hirsuta X-13 using alkaline lignin as the sole substrate, including the mycelial growth, activities of ligninolytic enzymes and the rates of lignin degradation and decolorization confirmed its great ligninolysis capacity. The maximum lignin degradation rate reached 39.8% after 11 days of T. hirsuta X-13 treatment, which was higher than that of reported fungi under the same condition. Fourier transform infrared spectrometry (FTIR), gas chromatography–mass spectrometry (GC–MS) scanning electron micrographs (SEM), two-dimensional heteronuclear single quantum coherence NMR analysis (2D-HSQC NMR) collaborated with pyrolysis gas chromatography–mass spectrometry (py-GC/MS) analyses proved that lignin structure was severely deconstructed along with amounts of monomer aromatics generated. Furthermore, according to those chemical analysis, in addition to canonical Cα–Cβ breakage, the cleavage of lignin interunit linkages of β–β might also occur by T. hirsuta X-13. Conclusions This study characterized a newly isolated white-rot basidiomycete T. hirsuta X-13 with impressive alkaline lignin degradation ability and provided mechanistic insight into its ligninolysis mechanism, which will be valuable for the development of lignin valorization strategies.

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Section: Resultssupporting

confidence: 68%

Elucidation of ligninolysis mechanism of a newly isolated white-rot basidiomycete Trametes hirsuta X-13

et al. 2021

Biotechnol Biofuels

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“…The most well-known phytosanitary problems on Paulownia spp. are witches' broom determined by phytoplasma (Gao et al, 2008), Phytophthora root and collar rot (Aloi et al, 2021), wood decay caused by Trametes hirsuta (Milenkovic et al, 2018) and root-knot nematodes (Skwiercz et al, 2019). Other diseases affecting Paulownia spp.…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity of Botryosphaeriaceae and Phytophthora species associated with Paulownia dieback, canker and root rot in Italy

BREGANT,

CARLONI,

BALESTRA

et al. 2023

Phytopathol. Mediterr.

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In recent years, an unusual decline and mortality has been observed in Paulownia plantations throughout the Marche region (Central Italy). Given the economic importance of this emerging forest crop, a study was conducted to determine which pathogens are directly involved in this syndrome. Field surveys performed in two plantations revealed the widespread occurrence of severe disease symptoms such as leaf chlorosis, crown thinning, shoot and branch dieback, sunken cankers, epicormic shoots and root rot. Disease incidence was also assessed by aerial remote sensing (RS) technologies using drones. Symptomatic samples collected from both stem and root tissues yielded fungal and fungal-like colonies representing two distinct families: Botryosphaeriaceae and Peronosporaceae. Morphological and DNA sequence data revealed five distinct species, identified as Macrophomina phaseolina and Botryosphaeria dothidea (Botryosphaeriaceae), Phytophthora pseudocryptogea, P. citrophthora and P. erythroseptica (Peronosporaceae). Given that all species are reported here for the first time on Paulownia, Koch’s postulates were satisfied inoculating the three Phytophthora species and two Botryosphaeriaceae at the collar of the stem of potted 1-year-old rooted cuttings in June 2023. Thirty days after inoculation, all plants showed the same symptoms as those observed in the field.

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“…There is little information on infectious diseases of paulownias and most of those reported so far are caused by fungi and oomycetes [4,[10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Phytophthora Root and Collar Rot of Paulownia, a New Disease for Europe

Aloi

Riolo

Spada

et al. 2021

Forests

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Paulownia species are fast growing trees native to China, which are being grown in managed plantings in several European countries for the production of wood and biomasses. In 2018, wilting, stunting, leaf yellowing, and collapse, as a consequence of root and crown rot, were observed in around 40% of trees of a 2-year-old planting of Paulownia elongata × P. fortunei in Calabria (Southern Italy). Two species of Phytophthora were consistently recovered from roots, basal stem bark, and rhizosphere soil of symptomatic trees and were identified as Ph. nicotianae and Ph. palmivora on the basis of both morphological characteristics and phylogenetic analysis of rDNA ITS sequences. Koch’s postulates were fulfilled by reproducing the symptoms on potted paulownia saplings transplanted into infested soil or stem-inoculated by wounding. Both Phytophthora species were pathogenic and caused root rot and stem cankers. Even though P. palmivora was the only species recovered from roots of naturally infected plants, in pathogenicity tests through infested soil P. nicotianae was more virulent. This is the first report of Phytophthora root and crown rot of a Paulownia species in Europe. Strategies to prevent this emerging disease include the use of healthy nursery plants, choice of well-drained soils for new plantations, and proper irrigation management.

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Decline of Paulownia tomentosa caused by Trametes hirsuta in Serbia

Cited by 9 publications

References 3 publications

Elucidation of ligninolysis mechanism of a newly isolated white-rot basidiomycete Trametes hirsuta X-13

Elucidation of ligninolysis mechanism of a newly isolated white-rot basidiomycete Trametes hirsuta X-13

Pathogenicity of Botryosphaeriaceae and Phytophthora species associated with Paulownia dieback, canker and root rot in Italy

Phytophthora Root and Collar Rot of Paulownia, a New Disease for Europe

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