Declined fitness in larvae born from long-distance migrants of anadromous &lt;i&gt;Coilia nasus&lt;/i&gt; in the lower reaches of the Yangtze River, China

Huang, Yanfei; Rypel, Andrew L.; Murphy, Brian R.; Xie, Shuguang

doi:10.24272/j.issn.2095-8137.2021.423

Cited by 2 publications

(2 citation statements)

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“…The estuarine tapertail anchovy ( Coilia nasus ), an important anadromous fish species, inhabits the Yangtze River in China. Juveniles grow in the brackish waters of the Yangtze River estuary, while adults migrate upstream to the middle and lower reaches of the river in early spring each year ( Huang et al, 2022 ). Spawning events occur gradually during migration as their ovaries mature.…”

Section: Introductionmentioning

confidence: 99%

Combining genome-wide association study and transcriptome analysis to identify molecular markers and genetic basis of population-asynchronous ovarian development in <i>Coilia nasus</i>

Yu,

Wan,

Huang

et al. 2024

Zoological Research

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Coilia nasus , a migratory fish species found in the middle and lower reaches of the Yangtze River and along offshore areas of China, possesses considerable aquacultural and economic potential. However, the species faces challenges due to significant variation in the gonadal development rate among females, resulting in inconsistent ovarian maturation times at the population level, an extended reproductive period, and limitations on fish growth rate due to ovarian prematurity. In the present study, we combined genome-wide association study (GWAS) and comparative transcriptome analysis to investigate the potential single nucleotide polymorphisms (SNPs) and candidate genes associated with population-asynchronous ovarian development in C. nasus . Genotyping of the female population based on whole-genome resequencing yielded 2 120 695 high-quality SNPs, 39 of which were suggestively associated with ovarian development. Of note, a significant SNP peak on LG21 containing 30 suggestively associated SNPs was identified, with cpne5a determined as the causal gene of the peak. Therefore, single-marker and haplotype association analyses were performed on cpne5a , revealing four genetic markers ( P <0.05) and seven haplotypes (r 2 >0.9) significantly associated with the phenotype. Comparative transcriptome analysis of precociously and normally maturing individuals screened out 29 and 426 overlapping differentially expressed genes in the brain and ovary, respectively, between individuals of different body sizes. Integrating the GWAS and transcriptome analysis results, this study identified genes and pathways related to hypothalamic-pituitary-gonadal axis hormone secretion, extracellular matrix, angiogenesis, and gap junctions involved in population-asynchronous ovarian development. The insights gained from this study provide a basis for a deeper understanding of the molecular mechanisms underlying ovarian development in fish and may facilitate the genetic breeding of C. nasus strains exhibiting population-synchronous ovarian development in the future.

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Section: Introductionmentioning

confidence: 99%

Combining genome-wide association study and transcriptome analysis to identify molecular markers and genetic basis of population-asynchronous ovarian development in <i>Coilia nasus</i>

Yu,

Wan,

Huang

et al. 2024

Zoological Research

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“…C. nasus is an important fish species in China’s Yangtze River because of its nutritional value and delicate taste [ 15 ]. In recent years, C. nasus has nearly become extinct due to overfishing and changes in aquatic ecology [ 16 ]. Very little research progress has been made on its artificial breeding [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Coilia nasus Spermatogonial Stem Cell Line Capable of Spermatogenesis In Vitro

Gu,

Zhang,

Zhong

et al. 2023

Biology

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The process by which spermatogonial stem cells (SSCs) continuously go through mitosis, meiosis, and differentiation to produce gametes that transmit genetic information is known as spermatogenesis. Recapitulation of spermatogenesis in vitro is hindered by the challenge of collecting spermatogonial stem cells under long-term in vitro culture conditions. Coilia nasus is a commercially valuable anadromous migrant fish found in the Yangtze River in China. In the past few decades, exploitation and a deteriorating ecological environment have nearly caused the extinction of C. nasus’s natural resources. In the present study, we established a stable spermatogonial stem cell line (CnSSC) from the gonadal tissue of the endangered species C. nasus. The cell line continued to proliferate and maintain stable cell morphology, a normal diploid karyotype, and gene expression patterns after more than one year of cell culture (>80 passages). Additionally, CnSSC cells could successfully differentiate into sperm cells through a coculture system. Therefore, the establishment of endangered species spermatogonial stem cell lines is a model for studying spermatogenesis in vitro and a feasible way to preserve germplasm resources.

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Declined fitness in larvae born from long-distance migrants of anadromous <i>Coilia nasus</i> in the lower reaches of the Yangtze River, China

Cited by 2 publications

References 1 publication

Combining genome-wide association study and transcriptome analysis to identify molecular markers and genetic basis of population-asynchronous ovarian development in <i>Coilia nasus</i>

Combining genome-wide association study and transcriptome analysis to identify molecular markers and genetic basis of population-asynchronous ovarian development in <i>Coilia nasus</i>

Establishment of a Coilia nasus Spermatogonial Stem Cell Line Capable of Spermatogenesis In Vitro

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